The Effect of Ca and Antidiuretic Hormone on Na Transport across Frog Skin

Curran, Peter F.; Herrera, Flor; Flanigan, William J.

doi:10.1085/jgp.46.5.1011

Cited by 167 publications

(81 citation statements)

References 12 publications

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“…The control values of the influx (Table 1) were somewhat larger than those found by Zadunaisky, Candia & Chiarandini (1963) in the same frog (31t-equiv hr-l cm-2) and Curran et al (1963) in Rana pipiens (1-40 p. equiv hr-' cm-2). The effect of ADH was tested at two different concentrations of Curran et al (1963) in R. pipiens.…”

Section: Na Influxmentioning

confidence: 55%

“…The abdominal skin of the South American frog Leptodactylus ocellatus L. was mounted in a lucite chamber of the type designed by Curran et al (1963). The electrical potential difference was measured by connecting the chambers through agar-Ringer bridges to calomel half cells and these cells to a Keithley 200B DC electrometer.…”

Section: Methodsmentioning

confidence: 99%

“…The effect of ADH was tested at two different concentrations of Curran et al (1963) in R. pipiens. The increase of sodium influx is not as large as the one elicited by the hormone in urinary bladder (Leaf & Dempsey, 1960;Bourguet & Morel, 1967;Bastide & Jard, 1968;Bourguet & Maetz, 1961).…”

Section: Na Influxmentioning

confidence: 99%

“…As discussed below, the entrance of Na into the epithelium across the outer barrier has always been regarded as a rate limiting step in the overall process of Na transport across epithelial membranes (Civan & Frazier, 1968;Curran & Gill, 1962;Curran et al 1963;Frazier, Dempsey & Leaf, 1962). Comparison of control data in Tables 1 and 2, though, indicates that JOT is larger than the transepithelial influx under all comparable conditions tested.…”

Section: Na Influxmentioning

confidence: 99%

“…Curran & Gill (1962) and suggested that the process of Na transport across frog skin was rate limited by the flux of Na from the outer bathing solution across a Na-selective barrier (JOT), and that the enhancement of Na transport elicited by antidiuretic hormones (ADH) was due to an increase of the permeability of this barrier. Curran, Herrera & Flanigan (1963) later developed a kinetic technique for the evaluation of the rate coefficient for 24Na movement across the individual barriers of the Na-transporting compartment, and demonstrated that ADH does in fact increase the Na permeability of the outer barrier. This conclusion can also be reached on the basis of the study of Na movement across urinary bladder (Leaf & Dempsey, 1960;Bastide & Jard, 1968) and is supported by the measurement of electrical parameters (Civan & Frazier, 1968).…”

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confidence: 99%

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The effect of antidiuretic hormone on Na movement across frog skin

Cereijido¹,

Rotunno²

1971

The Journal of Physiology

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SUMMARY1. The effect of antidiuretic hormone (ADH) on the movement and distribution of Na was studied. This was done using three different approaches: (a) the measurement of Na and 22Na in slices of epithelium of skins which were exposed to Ringer of varied composition containing 22Na, (b) the measurement of the influx of Na from the outer to the inner bathing solution with 22Na added to the outside, and (c) the use of a recently introduced technique which permits the direct evaluation of the flux from the outer solution -÷ epithelium, (JOT), i.e. the flux across the barrier which is generally regarded as the site of ADH activity.2. ADH increased the influx from the outer to the inner bathing solution of Na (50 %) not only when the concentration of Na on the outside was 115 mM (i.e. higher than in the epithelium) but even when the concentration was 1 mM (67 %).3. When the skin was bathed with 1mM-Na Ringer on the outside, ADH increased the unidirectional Na flux JOT by 56 % (Rana pipiens) and 71 % (Leptodactylus ocellatus). When the concentration was 115 mm a small increase (17 %) was observed in paired skins of R. pipiens. Under this condition no change was observed in L. ocellatus. 4. The amount of epithelial sodium which is labelled by 22Na added to the outside was taken to reflect the amount of Na involved in Na transport across the epithelium. Depending on whether the concentration of Na on the outside was high (115 mM) or low (1 mM), ADH produced an increase, or a decrease, of both the total Na content and the amount of 22Na exchanged.5. When the concentration of Na on the outside was low, ADH increased the total influx and JOT in spite of the fact that it lowers the total Na content and does not affect the exchangeable pool of Na. This observation is inconsistent with the view that the effect of ADH is due to the fact M. CEREIJIDO AND C. A. ROTUNNO that the increased permeability of the outer barrier allows more Na into the cell, and that the resulting increase of Na concentration in the cytoplasm accelerates the Na pumps at the inner side of the cells.6. It is concluded that ADH speeds up Na movements at the outward facing barrier, and that this exchange which facilitates the penetration of Na into a transporting compartment produces also a gain or a loss of Na in compartments not directly involved in Na transport across the epithelium. One compartment which is not involved in Na transport might be the cytoplasm of the epithelial cells.

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Section: Na Influxmentioning

confidence: 55%

Section: Methodsmentioning

confidence: 99%

Section: Na Influxmentioning

confidence: 99%

Section: Na Influxmentioning

confidence: 99%

mentioning

confidence: 99%

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The effect of antidiuretic hormone on Na movement across frog skin

Cereijido¹,

Rotunno²

1971

The Journal of Physiology

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Clinical Disorders of Urine Concentration and Dilution

Harrington¹

1973

Arch Intern Med

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Stochastische Modelle in der Nierenphysiologie

Fuchs

1969

Biom. J.

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Die Mere wird ihrer Aufgabe, nberfuhrung bestimmter chemischer Substanzenim allgemeinen Restprodukte von Stoffwechselvorgiingen, sogenannte harnpflichtige Stoffeaus dem Plasmaraum in den Urinraum durch zwei Faktoren gerecht : einen anatomischen und einen funktionellen. Anatomisch wird ein Stoffubertritt begiinstigt, indem beide Flussigkeitsraume in der Niere ein feinstverzweigtes Netz von kleinsten Kaniilen (Kapillaren und Tubuli) bilden, die, dicht ineinander verzahnt, so angeordnet sind, daB auf ganze Strecken Blut-und Harnkanalchen parallel nebeneinander herlaufen, nur durch eine Zellschicht voneinander getrennt. Funktionell sind die Zellen dieser Zwischenschicht fiihig, bestimmte Stoffe in einer oder beiden Richtungen passieren zu lassen, wobei als treibende Kriifte in erster Linie osmotische Kriifte, d. h. Konzentrationsgefiille, aber auch osmotische Mitnahmeeffekte in Frage kommen, daneben aber, z. B. bei Ionen, elektrische Kriifte und schlieBlich sogenannte aktive Transportvorglinge, die ihren Energiebedarf &us intrazellularen Stoffwechselvorgiingen decken, eine Rolle spielen. Zur Analyse dieser einzelnen biophysikalischen Effekte an den verschiedenen histologischen Abschnitten des Nierenparenchyms ist es notwendig, die einzelnen Substanzen in bekannter Konzentration in bestimmte Abschnitte der mikroskopisch kleinen intrarenalen Harnwege einzubringen und ihre Konzentration als Funktion von Ort und Zeit dort, aber ebenso im Plasmaraum und, wenn moglich, auch innerhalb der Zellen genugend genau zu bestimmen. Erst in den letzten Jahrzehnten ist dieses experimentelle Problem durch Einfuhrung der Mikropunktionstechnik sowie der Ultramikroanalyse losbar geworden, und von vorerst zwar nur wenigen, auf diesem Gebiet besonders gut ausgestatteten und eingearbeiteten Instituten wird eine immer grol3ere Zahl solcher Konzentrationsangaben fur einzelne Substanzen an einzelnen Nierenabschnitten unter definierten Versuchsbedingungen gewonnen. Damit wird fur den Biomathematiker die Aufgabe wichtig, aus Modellanalogien

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The Effect of Ca and Antidiuretic Hormone on Na Transport across Frog Skin

Cited by 167 publications

References 12 publications

The effect of antidiuretic hormone on Na movement across frog skin

The effect of antidiuretic hormone on Na movement across frog skin

Clinical Disorders of Urine Concentration and Dilution

Stochastische Modelle in der Nierenphysiologie

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