The effect of cadmium loading on protein synthesis function and functional state of laying hens’ liver

Gutyj, B. V.; Ostapiuk, Aleksandra; Kachmar, N.; Stadnytska, Olha; Sobolev, Olga; Binkevych, V; Petryshak, R.; Petryshak, O. I.; Kulyaba, O.; Naumyuk, A.; Nedashkivsky, V.; Nedashkivska, N.; Magrelo, N.; Golodyuk, I. P.; Nazaruk, N. V.; Binkevych, O

doi:10.15421/2019_82

Cited by 12 publications

(7 citation statements)

References 17 publications

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“…Останніми роками велика увага приділяється вивченню впливу шкідливих факторів навколишнього середовища на продуктивність тварин (Lavryshyn et al, 2018;Gutyi et al, 2019). Серед шкідливих екзогенних факторів, що негативно впливають на організм тварин, за даними багатьох дослідників негативну роль відіграють важкі метали як пріоритетні забруднювачі навколишнього середовища (Gutyi et al, 2018;Ostapyuk & Gutyj, 2019).…”

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Effect of sodium selenite and feed additive “Metisevit plus” on morphological parameters of blood of rats at the intoxication of Cadmium and Lead

2020

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Heavy metals, Lead and Cadmium, are hazardous environmental pollutants. In the body of mammals, Cadmium and Lead have a toxic effect on a number of organs and systems. The mechanism of action of these poisons is to inhibit the activity of enzyme systems by blocking carboxyl, amine and SH groups of protein molecules. The aim of the study was to investigate the effect of sodium selenite and feed additive “Metisevit plus” on the morphological parameters of rat blood by Cadmium and Lead intoxication. The experiments were carried out on 200–220 g male Wistar rats, out of which 3 groups of animals were formed: a control and two experimental animals. Rats in the control and experimental groups were administered 16.6 % aqueous lead acetate at a dose of 100 mg/kg and 0.029 % aqueous cadmium chloride at a dose of 2.0 mg/kg. The rats of the first experimental group additionally used sodium selenite. The rats of the second experimental group additionally used the feed additive “Metisevit plus”. Lead-cadmium loading in rats was accompanied by erythrocytopenia and leukopenia. The lowest erythrocyte count was in the blood of diseased rats at 21 days, where it decreased by 41.3 %, respectively, compared to the initial values. It was found that hemoglobin level decreased to 90.3 ± 2.63 g/l during Cadmium and Lead intoxication in control rats. At the same time as the decrease in hemoglobin level and the number of erythrocytes in intoxicated rats, an increase in the average hemoglobin content in one erythrocyte of blood was observed up to 20.57 ± 1.20 pg As a result of the development of lead-cadmium intoxication in ill rats, leukopenia was noted, which, in our opinion, is associated with systemic lesions of cadmium and lead in the blood-forming organs of animals that suppress leukopoiesis. It was found that the number of leukocytes in patients of rats of group C decreased by 36.1 % compared with the initial data. The use of sodium selenite and the feed additive “Metisevit plus” in rats under lead-cadmium loading contributed to the increase in the number of red blood cells and hemoglobin level, as well as the decrease in the number of leukocytes in their blood. The improvement of normalizing effect on the morphological parameters of blood of rats under conditions of loading with heavy metals was shown by the feed additive “Metisevit plus” in comparison with sodium selenite.

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Section: вступunclassified

Effect of sodium selenite and feed additive “Metisevit plus” on morphological parameters of blood of rats at the intoxication of Cadmium and Lead

2020

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“…First of all, this is due to the direct participation of the liver in the biotransformation of drugs and the rapid development of the pharmaceutical market. In particular, the literature mentions a significant amount of data demonstrating the involvement of liver monooxygenases in their metabolism (Cong et al, 2016;Gutyi et al, 2019;John & Kale, 2019). Depletion of the cytochrome P450 system involved in drug transformation can delay the elimination of toxic components of exogenous origin, cause them to accumulate in organs and tissues, or cause the formation of secondary substances, often even more toxic than the parent product.…”

Section: Introductionmentioning

confidence: 99%

Marker changes of blood plasma proteinogram in rats with toxic hepatitis

Gryshchenko

Minina

2020

Regul. Mech. Biosyst.

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In recent years, there has been a pronounced tendency to increase in the incidence of drug-induced liver damage due to the growing expansion of the pharmaceutical market, which is also observed in the case of incorrect administration of nonsteroidal anti-inflammatory drugs (NSAIDs). In this case, the violation of the functional state of the body has a negative effect on synthetic processes, which in combination with the protein system of tissues significantly affects the metabolic homeostasis of the body. Therefore, the aim of the study was to determine marker changes in the plasma protein spectrum in laboratory rats with diclofenac-induced hepatitis and the effectiveness of reparative therapy based on milk phospholipids. The drug form of toxic hepatitis in laboratory animals was induced according to the author’s model by oral administration of diclofenac sodium (NSAID group) at a dose of 12.5 mg/kg, once a day for 14 days. Thus, in rats with toxic hepatitis there was a probable decrease in plasma total protein content by 15.6% compared with control, indicating a violation of protein-synthesizing function of the liver. With the introduction into the body of clinically healthy and sick animals of the liposomal form of the bioadditive "FLP-MD" based on milk phospholipids, the level of total protein in blood plasma corresponded to control values. As a result of the study of the plasma protein spectrum of Wistar rats, the four most sensitive indicators, which undergo significant probable changes in absolute and relative units of measurement with the development of toxic diclofenac-induced hepatitis, are protein fractions with molecular weights of 180–190, 150–170, 60 and 54–58 kDa and four markers of the effectiveness of restoring the protein-synthesizing function of the liver with the use of corrective therapy, in particular, bioadditives "FLP-MD" – 900, 180–190, 68–70 kDa and the value of A/G ratio, which is important for implementation in applied veterinary medicine, especially in the diagnosis of NSAID hepatopathy, supplementing the picture of its pathogenesis at the molecular level and testing the effectiveness of newly created drugs of hepatoprotective profile.

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“…According to the results of numerous studies are conducted on various species of farm animals and poultry that selenium has antioxidant, anti-carcinogenic, radio protective, immunostimulatory, antiviral and antitoxic properties. There is direct evidence of the effect of selenium on osteogenesis, reproductive function, growth and development processes (Surai & Fisinin, 2014;Gutyj et al, 2019;Ashraf et al, 2019). Thus from the standpoint of modern ideas the normal functioning of the poultry's body and its full implementation of the genetic potential impossible without the presence of selenium in the diet.…”

Section: Introductionmentioning

confidence: 99%

Chemical composition, energy and biological value of broiler chicken meat caused by various doses of selenium

Sobolev¹,

Gutyj²,

Sobolieva³

et al. 2019

Ukr J Ecol

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From the standpoint of modern ideas, the normal functioning of the poultry body and full implementation her of the genetic potential is impossible without the presence of selenium in the diet. The biochemical diversity of selenium puts it in a number of priority trace elements. Scientists who were studying the effects of selenium on poultry body and paid relatively little attention to the quality of the meat. In scientific experiment are studied the effect of additives different doses of selenium in compound feed on the chemical composition, energy and biological value of meat of the cross chickens-broilers COBB 500. Introduction of selenium into the feed for broiler chickens in the doses which are studied (0.3 mg/kg, 0.4 and 0.5 mg/kg) did not significantly affect to the quality of their muscle tissue although it positively affected on some indicators that characterizing its chemical composition, nutritional and biological value. Among the experimental groups, the best meat quality indicators were in the second group of chickens for which was introduced into the feed selenium from the calculation of 0.3 mg/kg. When feeding mixed feeds with selenium additives to broiler chickens it was found that it is likely to increase the concentration of this trace element in muscle tissue by 60.6-100% (P?0.001), which does not exceed the maximum permissible level (MPL). Consumption of selenium-enriched meat of broiler chickens within the limits of the physiological norms recommended in Ukraine will ensure the daily requirement of an adult in this trace element by 23.6-29.4%. This meat product can be especially useful for people who live in regions with low levels of selenium in the natural environment.

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The effect of cadmium loading on protein synthesis function and functional state of laying hens’ liver

Cited by 12 publications

References 17 publications

Effect of sodium selenite and feed additive “Metisevit plus” on morphological parameters of blood of rats at the intoxication of Cadmium and Lead

Effect of sodium selenite and feed additive “Metisevit plus” on morphological parameters of blood of rats at the intoxication of Cadmium and Lead

Marker changes of blood plasma proteinogram in rats with toxic hepatitis

Chemical composition, energy and biological value of broiler chicken meat caused by various doses of selenium

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