The effect of calnexin deletion on the expression level of PDI in Saccharomyces cerevisiae under heat stress conditions

Zhang, Huili; He, Jing; Ji, Yanyan; Kato, Akio; Song, Yue

doi:10.2478/s11658-007-0033-y

Cited by 6 publications

(4 citation statements)

References 26 publications

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“…In this study, the expression of protein disulfide isomerase A4 (PDIA4) was upregulated to promote correct protein folding. PDI may interact to recover part of the function of CNX, thus promoting the recovery and maintenance of normal lung function in yaks under high-altitude conditions 36 . The misfolded proteins in the ER lumen are recognized by luminal chaperones such as HSP40, NEF, GRP94 and BIP, which transfer misfolded proteins to the ER membrane for ubiquitin-dependent degradation (Fig.…”

Section: Discussionmentioning

confidence: 99%

Molecular mechanisms detected in yak lung tissue via transcriptome-wide analysis provide insights into adaptation to high altitudes

Guo

Zheng

et al. 2021

Sci Rep

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Due to their long-term colonization of and widespread distribution in plateau environments, yaks can serve as an ideal natural animal model for the adaptive evolution of other plateau species, including humans. Some studies reported that the lung and heart are two key organs that show adaptive transcriptional changes in response to high altitudes, and most of the genes that show differential expression in lung tissue across different altitudes display nonlinear regulation. To explore the molecular mechanisms that are activated in yak lung tissue in response to hypoxia, the mRNAs, lncRNAs and miRNAs of lung tissue from 9 yaks living at three different altitudes (3400 m, 4200 m and 5000 m), with three repetitions per altitude, were sequenced. Two Zaosheng cattle from 1500 m were selected as low-altitude control. A total of 21,764 mRNAs, 14,168 lncRNAs and 1209 miRNAs (305 known and 904 novel miRNAs) were identified. In a comparison of yaks and cattle, 4975 mRNAs, 3326 lncRNAs and 75 miRNAs were differentially expressed. A total of 756 mRNAs, 346 lncRNAs and 83 miRNAs were found to be differentially expressed among yaks living at three different altitudes (fold change ≥ 2 and P-value < 0.05). The differentially expressed genes between yaks and cattle were functionally enriched in long-chain fatty acid metabolic process and protein processing, while the differentially expressed genes among yaks living at three different altitudes were enriched in immune response and the cell cycle. Furthermore, competing endogenous RNA (ceRNA) networks were investigated to illustrate the roles of ceRNAs in this process, the result was also support the GO and KEGG analysis. The present research provides important genomic insights for discovering the mechanisms that are activated in response to hypoxia in yak lung tissue.

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Section: Discussionmentioning

confidence: 99%

Molecular mechanisms detected in yak lung tissue via transcriptome-wide analysis provide insights into adaptation to high altitudes

Guo

Zheng

et al. 2021

Sci Rep

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“…It appears that the deletion of calnexin results in the induction of the unfolded protein response (UPR), and increases the levels of PDI and BiP to fold the unfolded glycosylated lysozymes (internal stress) [13]. We found that the induction of PDI in the ER could recover part of the function of calnexin in calnexin-disrupted yeast under heat stress conditions [14]. BiP, unlike PDI, which directly interacts with calnexin, was also found to cooperate with calnexin in the ATP-dependent refolding of glycoprotein and non-glycosylated substrates [15].…”

Section: Introductionmentioning

confidence: 83%

The effect of calnexin deletion on the expression level of binding protein (BiP) under heat stress conditions in Saccharomyces cerevisiae

Zhang

et al. 2008

Cellular and Molecular Biology Letters

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Abstract:In order to investigate the effect of calnexin deletion on the induction of the main ER molecular chaperone BiP, we cultured the wild-type and calnexin-disrupted Saccharomyces cerevisiae strains under normal and stressed conditions. The growth rate of the calnexin-disrupted yeast was almost the same as that of the wild-type yeast under those conditions. However, the induced level of BiP mRNA in the ER was evidently higher in calnexin-disrupted S. cerevisiae than in the wild-type at 37ºC, but was almost the same in the two strains under normal conditions. The Western blot analysis results for BiP protein expression in the ER showed a parallel in the mRNA levels in the two strains. It is suggested that under heat stress conditions, the induction of BiP in the ER might recover part of the function of calnexin in calnexin-disrupted yeast, and result in the same growth rate as in wild-type yeast.

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“…Как уже было сказано, Pdi -мультифункциональный белок и может действовать в клетке и как фермент, и как шаперон. Есть данные, что Pdi в условиях теплового стресса может брать на себя часть функций шаперона ЭР -калнексина [43]. Полученные данные позволяют предположить, что повышенная продукция Pdi оказывает влияние на различные внутриклеточные процессы.…”

Section: Discussionunclassified

Influence of PDI gene overexpression on heterological proteins production in yeast Pichia pastoris

Tsygankov

Александрович²,

Падкина

et al. 2017

Ecological genetics

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Background. The yeast Pichia pastoris is used for synthesis of recombinant secretory proteins. Overexpression of assistant genes, coding proteins involved in secretion, is one of approaches to improve the production of target protein. PpPDI gene encodes P. pastoris yeast protein disulfide isomerase (Pdi). The aim of our study was to evaluate the effect of Pdi overproduction on recombinant interferons (human interferon-alfa16 and chicken interferon-gamma) production. Materials and Methods. PpPDI gene was cloned under the control of the AOX1 gene promoter in plasmid pPICZαA. Primers for AJ302014.1 nucleotide sequence of NCBI data base were used for PpPDI gene cloning. The chromosomal DNA of the GS115 strain was used as a template. To generate strains with PpPDI gene overexpression we used a previously obtained strains producing human interferon-alfa16 and chicken interferon-gamma. Yeast transformation was performed by electroporation. Cultivation was performed using single and two-stage strategies in standard media containing methanol as the sole carbon source to induce the AOX1 gene promoter. Results. We obtained interferon-producing strains with PpPDI gene overexpression. Over-expression of the PpPDI gene in yeast P. pastoris increases the production of interferon-alfa16, a protein containing disulfide bonds, regardless of the mode of cultivation. Effect of PpPDI gene over-expression on the production of interferon-gamma - the protein without disulfide bonds, depends on cultivation mode. Conclusion. PpPDI gene overexpression can be used to enhance the production of interferons and other proteins that contain disulfide bonds. Effect of PpPDI gene overexpression on recombinant proteins without disulfide bonds may depend on cultivation procedure.

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The effect of calnexin deletion on the expression level of PDI in Saccharomyces cerevisiae under heat stress conditions

Cited by 6 publications

References 26 publications

Molecular mechanisms detected in yak lung tissue via transcriptome-wide analysis provide insights into adaptation to high altitudes

Molecular mechanisms detected in yak lung tissue via transcriptome-wide analysis provide insights into adaptation to high altitudes

The effect of calnexin deletion on the expression level of binding protein (BiP) under heat stress conditions in Saccharomyces cerevisiae

Influence of PDI gene overexpression on heterological proteins production in yeast Pichia pastoris

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