The Effect of Carbodiimide on the Structural, Mechanical and Biological Properties of Collagen Films

Nashchekina, Yu. A.; Sirotkina, M. Yu.; Darvish, Diana; Barsuk, Ilya; Moskalyuk, O. A.; Mikhailova, N. A.

doi:10.1134/s1990519x21060079

Cited by 6 publications

(3 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In a study performed by Nashchekina et al, the modification by EDC/NHS resulted in a reduction in films' elasticity. This reduction was proportional to the concentration of the crosslinking agent used: as the crosslinker concentration increased, the films became less elastic [34]. The samples achieved maximum swelling degree approximately two hours after immersion in the PBS solution.…”

Section: Discussionmentioning

confidence: 98%

The Influence of Various Crosslinking Conditions of EDC/NHS on the Properties of Fish Collagen Film

Sionkowska,

Kulka-Kamińska,

Brudzyńska

et al. 2024

Marine Drugs

View full text Add to dashboard Cite

The process of crosslinking improves the physicochemical properties of biopolymer-based composites, making them valuable for biomedical applications. EDC/NHS-crosslinked collagen materials have a significant potential for tissue engineering applications, due to their enhanced properties and biocompatibility. Chemical crosslinking of samples can be carried out in several ways, which is crucial and has a direct effect on the final properties of the obtained material. In this study, the effect of crosslinking conditions on the properties of collagen films using EDC and NHS was investigated. Studies included FTIR spectroscopy, AFM, swelling and degradation tests, mechanical testing and contact angle measurements. Evaluation of prepared collagen films indicated that both crosslinking agents and crosslinking conditions influenced film properties. Notable alternations were observed in the infrared spectrum of the sample, to which EDC was added directly to the fish collagen solution. The same sample indicated the lowest Young modulus, tensile strength and breaking force parameters and the highest elongation at break. All samples reached the maximum swelling degree two hours after immersion in PBS solution; however, the immersion-crosslinked samples exhibited a significantly lower degree of swelling and were highly durable. The highest roughness was observed for the collagen film crosslinked with EDC, whereas the lowest was observed for the specimen crosslinked with EDC with NHS addition. The crosslinking agents increased the surface roughness of the collagen film, except for the sample modified with the addition of EDC and NHS mixture. All films were characterized by hydrophilic character. The films’ modification resulted in a decrease in their hydrophilicity and wettability. Our research allows for a comparison of proposed EDC/NHS crosslinking conditions and their influence on the physicochemical properties of fish collagen thin films. EDC and NHS are promising crosslinking agents for the modification of fish collagen used in biomedical applications.

show abstract

Section: Discussionmentioning

confidence: 98%

The Influence of Various Crosslinking Conditions of EDC/NHS on the Properties of Fish Collagen Film

Sionkowska,

Kulka-Kamińska,

Brudzyńska

et al. 2024

Marine Drugs

View full text Add to dashboard Cite

show abstract

“…Методы химической сшивки коллагена основаны на наличии реакционноспособных концевых или боковых амино-и карбоксильных групп. В качестве сшивающих агентов обычно используются альдегиды, генипин и карбодиимиды [4][5][6][7][8][9][10]. Однако зачастую подобные методы не подходят для получения гидрогелей с инкапсулированными клетками [11] ввиду токсичности подобных сшивающих агентов.…”

Section: Introductionunclassified

New smart thermosensitive biomaterials on the basis of collagen modified by ethylene glycol vinyl glycidyl ether for 4D bioprinting

Farion,

Buinov,

Nikishina

et al. 2023

Izvestiâ vuzov. Prikladnaâ himiâ i biotehnologiâ

View full text Add to dashboard Cite

In this study, collagen reactive under UV radiation was obtained for the first time via the functionalization of the side amino groups of lysine moieties with unsaturated ethylene glycol vinyl glycidyl ether (Vinylox) containing, similarly to glycidyl methacrylate, an epoxy group. The presence of this group having a moderate reactivity provides selective grafting of the side amino groups of collagen in a neutral or weakly alkaline medium and excludes the formation of by-products in the Michael reaction and hydrolysis characteristic of the analog—glycidyl methacrylate. Photometry data indicate that with modification in a weakly basic aqueous solution at room temperature and a significant excess of Vinylox, a grafting degree of 23.4% can be achieved. For the first time, film-forming composites capable of photocuring were obtained by adding polyethylene glycol diacrylate and poly(N-isopropylacrylamide) to a weakly acidic aqueous solution of collagen. The presence of vinyl oxide groups provided satisfactory mechanical characteristics of films as the result of UV-initiated collagen cross-linking, while the presence of poly(N-isopropylacrylamide) ensured hydrophilic-hydrophobic smart sensitivity. The films have a well-developed fibrillar structure, and the size of voids enables the free movement of nutrients and other compounds. According to MTT assay data, the films release no cytotoxic components and maintain the metabolic activity of stem cells, providing sufficient density of stem cells on their surface. All of the factors mentioned above determine the prospects of using the films both as an artificial extracellular matrix scaffold and as smart thermosensitive scaffolds used to grow stem cells on their surfaces for subsequent laser transfer bioprinting.

show abstract

“…The stability of such hydrogels can be increased, and the desorption of HA can be reduced by obtaining a cross-linked collagen network. Cross-linking agents such as carbodiimide are widely used [22]. Other authors used PEG ester tetrasuccinimidyl glutarate for cross-linking, which interacts with collagen amino groups to produce a stable semi-permeable network hydrogel to survive the chondrocytes [23].…”

Section: Introductionmentioning

confidence: 99%

Effect of Phytic Acid Addition on the Structure of Collagen-Hyaluronic Acid Composite Gel

Nashchekina,

Guryanov,

Lihachev

et al. 2023

Gels

View full text Add to dashboard Cite

Composite collagen gels with hyaluronic acid are developed tissue-engineered structures for filling and regeneration of defects in various organs and tissues. For the first time, phytic acid was used to increase the stability and improve the mechanical properties of collagen gels with hyaluronic acid. Phytic acid is a promising cross-linker for collagen hydrogels and is a plant-derived antioxidant found in rich sources of beans, grains, and oilseeds. Phytic acid has several benefits due to its antioxidant, anticancer, and antitumor properties. In this work, studies were carried out on the kinetics of the self-assembly of collagen molecules in the presence of phytic and hyaluronic acids. It was shown that both of these acids do not lead to collagen self-assembly. Scanning electron microscopy showed that in the presence of phytic and hyaluronic acids, the collagen fibrils had a native structure, and the FTIR method confirmed the chemical cross-links between the collagen fibrils. DSC and rheological studies demonstrated that adding the phytic acid improved the stability and modulus of elasticity of the collagen gel. The presence of hyaluronic acid in the collagen gel slightly reduced the effect of phytic acid. The presence of phytic acid in the collagen gel improved the stability of the scaffold, but, after 1 week of cultivation, slightly reduced the viability of mesenchymal stromal cells cultured in the gel. The collagen type I gel with hyaluronic and phytic acids can be used to replace tissue defects, especially after the removal of cancerous tumors.

show abstract

The Effect of Carbodiimide on the Structural, Mechanical and Biological Properties of Collagen Films

Cited by 6 publications

References 20 publications

The Influence of Various Crosslinking Conditions of EDC/NHS on the Properties of Fish Collagen Film

The Influence of Various Crosslinking Conditions of EDC/NHS on the Properties of Fish Collagen Film

New smart thermosensitive biomaterials on the basis of collagen modified by ethylene glycol vinyl glycidyl ether for 4D bioprinting

Effect of Phytic Acid Addition on the Structure of Collagen-Hyaluronic Acid Composite Gel

Contact Info

Product

Resources

About