The effect of carbon dioxide availability on succinic acid production with biofilms of  Actinobacillus succinogenes

Herselman, Jolandi; Bradfield, M.F.A.; Vijayan, Uma Rajendra Prasad; Nicol, Willie

doi:10.1016/j.bej.2016.10.018

Cited by 31 publications

(12 citation statements)

References 21 publications

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“…Actinobacillus succinogenes can metabolize a broad spectrum of carbon sources, including C5 and C6 sugars, disaccharides, and other carbon sources, such as mannitol, glycerol, cane molasses, whey, wheat, and corn-stover hydrolysates (Table 2). [35][36][37][38][39][40] Substrate cost generally accounts for around 50% in biotechnological processes. The adoption of low-cost substrates for succinic acid production is one of the critical parameters to make the bioprocess more economical.…”

Section: Wide Carbon Utilization Spectrum Of a Succinogenesmentioning

confidence: 99%

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Comprehensive investigation of succinic acid production by Actinobacillus succinogenes: a promising native succinic acid producer

Qiao

Dai

et al. 2019

Biofuels Bioprod Bioref

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The biological production of succinic acid has attracted a great deal of attention due to the depletion of fossil fuels and concerns regarding environmental issues. As a natural producer of succinic acid, Actinobacillus succinogenes is one of the most promising candidates owning to its high production capability, wide carbon utilization spectrum, and robustness to environmental factors. This review details current achievements attempting to improve the efficiency of succinic acid production, not only in terms of raw materials and metabolic engineering strategies but also in terms of process optimization, such as work on neutralizing agents, redox potential, and the supply of CO2. Strategies to reduce the accumulation of byproduct accumulation and to improve succinic acid synthesis are discussed. © 2019 Society of Chemical Industry and John Wiley & Sons, Ltd.

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Section: Wide Carbon Utilization Spectrum Of a Succinogenesmentioning

confidence: 99%

“…Metabolic pathway for succinic acid productionIn A. succinogenes, glucose is first metabolized to phosphoenolpyruvate (PEP) by the glycolysis or oxidative pentose phosphate pathway. Then, PEP is converted into pyruvate, formate, acetate, and ethanol via the C 3 pathway or into succinic acid via the C 4 pathway36 …”

mentioning

confidence: 99%

Comprehensive investigation of succinic acid production by Actinobacillus succinogenes: a promising native succinic acid producer

Qiao

Dai

et al. 2019

Biofuels Bioprod Bioref

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“…One such potential bioprocess is the production of succinic acid (SA) platform chemical using bioreactors of Actinobacillus succinogenes biofilms. The production of SA with A. succinogenes has been subjected to intense research scrutiny in the open literature, exploring steady-state metabolic flux distributions at various fermenter conditions [2][3][4][5], rate comparisons of biofilm and suspended modes [3,5], fermentations of various sugar types including hydrolysate streams [6][7][8], and using different reactor configurations [3,9,10]. Maharaj et al [5] observed that specific succinic acid (SA) productivity (per mass of biomass) decreased with increasing concentrations of SA, which was attributed to possible changes in the composition of the biofilm or fraction of the metabolically active cells within the biofilm.…”

Section: Introductionmentioning

confidence: 99%

Structure and cell viability analysis of Actinobacillus succinogenes biofilms as biocatalysts for succinic acid production

Mokwatlo

Nicol

2017

Biochemical Engineering Journal

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Biofilms of Actinobacillus succinogenes have demonstrated exceptional capabilities as biocatalysts for high productivity, titre, and yield production of succinic acid. This work gauges the effectiveness of these biofilms through analysis of the structure and cell viability of biofilms by using confocal scanning laser microscopy, scanning electron microscopy and image analysis software. The structure of the mature six-day-old biofilms of A. succinogenes was characterized by amorphous cell microcolonies of variable area and shape, with a mean thickness of 92±35 µm and low surface area to volume ratios of 0.25±0.07 µm 2 µm-3. Biofilm surface area coverage was low near the base (33±13%) but increased towards the outer layer. Despite the presence of water channels at the deeper portions of the biofilm, a greater portion of inactive cells was closest to the attachment surface and 65±2% of the biofilm consisted of dead cells. In this way, a cell viability gradient existed where the outer layer exhibits a greater fraction of active cells compared to the base layer. Biofilm cells underwent a phenotypic change, expressing a filamentous cell morphology different from rod-like morphology of suspended cells. The filamentous morphology permits extensive cell entanglements within a microcolony which may add to the intactness of microcolonies.

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“…yields) between succinate, acetate and formate remained constant. Below 3.9 mM dissolved CO2 (or 17% saturation), the yield of succinate also decreased, as flux was diverted towards other products [113].…”

Section: Co2 Limitation In Succinic Acid Producersmentioning

confidence: 99%

“…By equalizing the energy charges of the different nucleotide pools, it will nullify any effects that a difference in energy charge would have, if indeed there is a difference. Worthwhile as well might be to accurately determine the concentration at which CO2 becomes limiting, as was done by Herselman et al (2017) [113], for a wide range of succinate producers that either rely on ATP, or GTP-dependent PEP carboxykinases, in order to see if there is a difference.…”

Section: Open Questions Regarding Pseudoclostridium Thermosuccinogenesmentioning

confidence: 99%

The thermophilic route to succinic acid

Koendjbiharie¹

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Chapter 1-General introduction and thesis outline Biotechnology and the biobased economy Many nuanced definitions exist for biotechnology that generally describe the use of living systems, organisms, or parts thereof in order to alter or generate products, systems, or environments to benefit people. As such, biotechnology is a very broad, and broadly used term that covers a plethora of different fields and technologies, ranging from ancient beer brewing and winemaking processes to the production of modern therapeutics using engineered eukaryotic cell-lines. Agriculture itself, however, is generally not considered biotechnology, even though it would fit the definition. Since about fifteen years ago, it has become common to distinguish between different types of biotechnology using colors: white (industrial) biotechnology, red (pharmaceutical) biotechnology, green (agro) biotechnology, and blue (marine) biotechnology are the most common distinctions that are used, but many more exist and they are not always used consistently. * Sugar cane/ Maize Malic anhydride 6.8 Bio succinic acid Today 1.3-3.6 / 2.3-4.6* Future 0.9-2.1 / 1.8-2.9* [226] Chemical DSP GJ/t (NREU) Comments Malic anhydride-67.7 * Sugar cane / Maize Petr. succinic acid-96.3 Bio succinic acid Direct crystallization 44.9 / 66.5* Natural and engineered succinic acid producers A large variety of organisms have been studied for their capacity to naturally produce succinic acid, and several natural and non-natural producers-both bacteria and eukaryotes-have been engineered to produce (more) succinic acid. The most-studied species are briefly described below. Bacteria Actinobacillus succinogenes A. succinogenes is a facultative anaerobic, pleomorphic, Gram-negative rod, first isolated from bovine rumen [103]. It is capnophilic (CO2-"loving") and can grow on a broad range of pentose and hexose sugars. It naturally produces succinic acid, up to 70 g/l with yields of General introduction 1 | 21 around 0.75 g/g sugar [178,249]. Succinic acid is formed via the reductive branch of the TCA cycle, which is bifurcated, with succinic acid as one of the end-points [102]. Anaerobiospirillum succiniciproducens A. succiniproducens is an anaerobic, Gram-negative, spiral shaped bacterium. It was first isolated from a dog, but is also present in humans and can-in rare cases-be the cause of bacteraemia or other types of opportunistic infections [67,131]. Similar to A. succinogenes, A. succiniproducens is a capnophile able to grow on a large range of substrates. The available CO2 concentration strongly influences the succinic acid production [250]. Yields of around 1.4 g/g glycerol have been reported [43,160]. Corynebacterium glutamicum C. glutamicum is a facultative anaerobic, Gram-positive rod. It has a long history for industrial-scale production of a variety of amino acids [310]. It is unable to grow under oxygen deprived conditions, but retains the possibility to ferment sugars allowing a process to shift easily from a biomass formation phase and a high-density production...

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The effect of carbon dioxide availability on succinic acid production with biofilms of Actinobacillus succinogenes

Cited by 31 publications

References 21 publications

Comprehensive investigation of succinic acid production by Actinobacillus succinogenes: a promising native succinic acid producer

Comprehensive investigation of succinic acid production by Actinobacillus succinogenes: a promising native succinic acid producer

Structure and cell viability analysis of Actinobacillus succinogenes biofilms as biocatalysts for succinic acid production

The thermophilic route to succinic acid

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