The Effect of Carprofen Treatment on Conception Rate After Insemination in Cows with Increased Days in Milk

Abstract

In this study the effect of carprofen treatment on conception rate after insemination in Holstein cows that did not conceive for a long time after parturition was evaluated. In the study, 200 Holstein cows with days in milk >120 were used. A progesterone + ovsynch-based estrus synchronization protocol was applied to the cows included in the study. For this purpose, the progesterone device was placed intravaginally together with GnRH injection. Seven days later, the progesterone source was removed from the vagina and PGF2α injection was administered. GnRH injection was administered 48 hours following PGF2α injection and fixed-time insemination was performed 12-16 hours later. After insemination, animals were randomly divided into 2 groups. Subcutaneous carprofen was applied to the carprofen group (n = 100) on the 14th day after insemination and physiological saline was applied to the control group (n = 100) on the same day. Three cows from the carprofen group and 5 cows from the control group were excluded from the study for various reasons. The pregnancy rate was 42.26% (41/97) in the carprofen group and 25.26% (24/95) in the control group (p <0.05). However, the rate of conception was found to be the lowest (10.52%) in cows with high milk yield (>30 kg) in the control group. In addition, it was found that carprofen administration to cows with high milk yield increased the rate of conception. However, it was determined that the days in milk had no effect on pregnancy rate. As a result, it was concluded that the administration of carprofen on the 14th day after insemination to cows who could not conceive for a long time after parturition may be effective in increasing the rate of conception.

Post‐mating diclofenac vs. carprofen treatment on serum progesterone levels and reproductive outcomes inHungarian‐Merinoewes during the non‐breeding season

“…Although an increase in pregnancy rates was observed with post‐mating double‐dose diclofenac application in goats (Aksu, 2019; Doğruer et al, 2012), no increase was observed in pregnancy rates in our study in ewes. Previous studies of carprofen treatment in dairy cows have reported varying degrees of positive responses differing from 12% (Özkök, 2019) to 17% (Alkan et al, 2021) in pregnancy rate. While some others have reported decrease at about 2.5% to 10% (Heuwieser et al, 2011; Satılmış et al, 2020; Von Krueger & Heuwieser, 2010), compared to the controls.…”

A Critical Overview on Prostaglandin Inhibitors and Their Influence on Pregnancy Results after Insemination and Embryo Transfer in Cows

“…Carprofen is a long effective NSAID with a clinical effect of 12 h. It has a small volume of distribution much longer plasma elimination half-life (30 to 40 h) in cattle than FM and is poorly excreted in milk [ 43 ]. Administration of CAR had no significant effect on the concentration of plasma progesterone or on embryo mortality [ 44 ], and when administered during the critical period of pregnancy, i.e., on the 14th and 15th Day after insemination, did not have a significant effect on the pregnancy rate in cows [ 16 , 45 , 46 ]. In cows (>120 days in milk), receiving CAR subcutaneously 14 days after timed AI, the PR ( p < 0.05) was 42.26% compared to 25.26% in the control group.…”

“…It is also worth of noting that in high-yield cows (>30 kg of milk per day), the PR was much lower (10.52%). The conclusion was that administration of CAR on the 14th Day after insemination to cows who could not conceive for a long time after parturition may be effective in increasing the rate of conception [ 46 ]. In the latest experiment of Abay et al [ 47 ], CAR administration 14 days after AI improved chances ( p < 0.05) of maintaining the CL in females with body condition score (BCS) ≤ 2.5 (81.7 vs. 70.7% respectively for CAR and control group), whereas for animals with BCS > 2.5 CAR treatment decreased CL maintaining chances compared to the control (74.5 vs. 90.5%, respectively, p < 0.05).…”