The Effect of CCC on Growth and Endogenous Growth Substances in Wedgwood Iris

Pereira, A. S. Rodrigues

doi:10.1111/j.1438-8677.1970.tb00193.x

Cited by 5 publications

(1 citation statement)

References 11 publications

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“…(10). These results provide a logical explanation for the reports (13,14) of increases in extractable gibberellins following growth retardant application. Since gibberellins and sterols share a common biosynthetic pathway as far as tarnesyl pyrophosphate, the inhibition of the sterol branch would increase the amount of precursor available for the gibberellin branch.…”

Section: Methodsmentioning

confidence: 52%

Inhibition of Sterol Biosynthesis by 2-Isopropyl-4-dimethylamino-5-methylphenyl-1-piperidine Carboxylate Methyl Chloride in Tobacco and Rat Liver Preparations

Douglas

Paleg

1972

Plant Physiol.

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(3,18), and mammals (9, 11), no one set of results satisfactorily accounts for the great diversity of organismic responses.When a group of compounds produces different results in different systems, it is important to assess carefully whether the effects recorded with one species are in any way pertinent to those recorded for other species. This paper presents evidence which strongly supports the contention that at least one retardant effect observed in cell-free rat liver systems has direct relevance to the effects of the retardant on higher plants. It is also the first report of the inhibition of sterol biosynthesis in plants by a growth retardant. MATERIALS AND METHODSPlant Tissue. Nicotiana tabacum (cv. Samson) seeds were germinated in potting compost and grown in a glasshouse at 18 to 24 C. Immediately before an experiment, when the seedlings were 2 to 3 cm in length (usually 3-4 weeks after germination), they were removed, thoroughly washed, and the roots were excised. A number of rootless seedlings (totaling about 300 mg in weight) were placed in a solution of 5 yC DL-2-'4C-mevalonic acid lactone in 11 mm phosphate buffer, pH 6.5, in 5-ml Petri dishes. Amo 1618,1 at a final concentration of 1 'Abbreviations: Amo 1618: 2-isopropyl-4-dimethylamino-5-methylphenyl-l-piperidine carboxylate methyl chloride, 90%; CCC: 2-chloroethyl-trimethyl ammonium chloride; SK & F 7997: tris(2-diethylaminoethyl)phosphate hydrochloride. mg/ml, was added to one set of Petri dishes, and the pH was readjusted to 6.5. Final volume in all treatments was 2 ml. The seedlings were placed around the edge of the dishes so that only the cut stems were immersed in the solution. The Petri dishes containing the seedlings were placed in a closed, clear plastic container and left under constant illumination (230 ft-c)for 24 hr at a constant temperature of 18 C.Extraction. After incubation, the seedlings were removed with forceps and homogenized in three successive 5-ml portions of ethanol-benzene (4:1), with an Ultra-turrax blender. The combined extracts were filtered through Whatman No. 1 filter paper and reduced to dryness in vacuo in a rotary evaporator.The residue was taken up in 5 ml of water, and the lipids were extracted with diethyl ether (4 X 5 ml). The combined ether extracts were washed with water (2 X 5 ml), and the ether fraction was evaporated in vacuo. The lipid residue was redissolved in 20 ml of hexane and extracted with 80% (v/v) methanol (3 X 20 ml) to remove acidic and polar lipids. The hexane was then removed in vacuo, and the residue was saponified under reflux with 5% (w/v) KOH in 85% (v/v) methanol (5 ml) for 60 min. All nonsaponifiable lipid material was then extracted with diethyl ether (3 X 10 ml), and the combined extracts were washed with water (5 ml). The ether was removed in vacuo, and the lipid residue was dissolved in benzene.Thin Layer Chromatography. Nonsaponifiable lipid fractions were spotted on thin layer plates in an atmosphere of dry nitrogen. An aliquot of the benzene solution was spotted on a Silica G...

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Section: Methodsmentioning

confidence: 52%

Inhibition of Sterol Biosynthesis by 2-Isopropyl-4-dimethylamino-5-methylphenyl-1-piperidine Carboxylate Methyl Chloride in Tobacco and Rat Liver Preparations

Douglas

Paleg

1972

Plant Physiol.

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The effect of acetylcholine on growth and on growth inhibition by CCC in wheat seedlings

Dekhuijzen¹

1973

Planta

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The inhibition of the growth of wheat seedlings by 2-chloroethyltrimethylammonium chloride (CCC) was strongly reduced by root application of acetylcholine (Ach). Ach was applied after uptake of CCC by the roots or by the leaves. Ach also stimulated growth of non-CCC-treated seedlings up to 30% when applied to the roots. Growth stimulation appeared to be dependent on pH of the medium and most effective at pH 4.5 and 6. At pH 7.5 Ach did not promote growth of wheat seedlings.

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1972

The Growth of Bulbs

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The Effect of CCC on Growth and Endogenous Growth Substances in Wedgwood Iris

Cited by 5 publications

References 11 publications

Inhibition of Sterol Biosynthesis by 2-Isopropyl-4-dimethylamino-5-methylphenyl-1-piperidine Carboxylate Methyl Chloride in Tobacco and Rat Liver Preparations

Inhibition of Sterol Biosynthesis by 2-Isopropyl-4-dimethylamino-5-methylphenyl-1-piperidine Carboxylate Methyl Chloride in Tobacco and Rat Liver Preparations

The effect of acetylcholine on growth and on growth inhibition by CCC in wheat seedlings

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