The effect of cell-based bone tissue engineering in a goat transverse process model

Kruyt, Moyo C.; Wilson, Clayton E.; Bruijn, Joost D. de; Blitterswijk, Clemens A. van; Oner, F. Cumhur; Verbout, Abraham J.; Dhert, W.J.A.

doi:10.1016/j.biomaterials.2006.05.048

Cited by 65 publications

(62 citation statements)

References 28 publications

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“…[15][16][17] BCPA and BCPB ceramics had similar chemical composition, crystallinity, and macrostructure, but their microstructure differed significantly. BCPA was highly microporous.…”

Section: Discussionmentioning

confidence: 99%

“…For implantation on the lumbar transverse processes, polymeric cassettes [15][16][17] were used. Each cassette housed two material conditions separated with a Teflon sheet.…”

Section: Animals and Implantationmentioning

confidence: 99%

“…For histomorphometry, high-resolution digital photographs (300 dpi) of the stained sections were made. 15,16 A program was developed to quantify bone formation parameters: percentage of bone occupying available pore area (%b/pore); percentage of available scaffold outline in contact with bone (%b.cont); and maximuml bone depth from the transverse process/implant contact (b.depth). Also, maximum depth of each fluorochrome label was measured for the transverse process implants and used to display bone migration dynamics.…”

Section: Animals and Implantationmentioning

confidence: 99%

“…13,14 We investigated the performance of six calciumphosphate ceramic based implants using an adapted transverse processes model. [15][16][17] The rationale was to distinguish the best performing material with regard to bone repair. A preselection was previously made based on physicochemical properties and screening in an intramuscular model, 18 four BCP and one CA ceramic were investigated.…”

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confidence: 99%

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Comparative in vivo study of six hydroxyapatite‐based bone graft substitutes

Habibović

Kruyt

Juhl

et al. 2008

Journal Orthopaedic Research

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214

186

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Improvement of synthetic bone graft substitutes as suitable alternatives to a patient's own bone graft remains a challenge in biomaterials research. Our goal was to answer the question of whether improved osteoinductivity of a material would also translate to better bone-healing orthotopically. Three porous biphasic calcium phosphate (BCP) ceramics (BCPA, BCPB, and BCPC), consisting of hydroxyapatite and b-tricalcium phosphate, a porous biphasic calcium phosphate ceramic reinforced with a bioresorbable polylactic acid to improve its mechanical properties (BCPCþ), a pure hydroxyapatite ceramic (HA), and a carbonated apatite ceramic (CA) were implanted intramuscularly and orthotopically by using a transverse process model in 11 goats for 12 weeks. BCPA and BCPB had similar chemical composition but differed in their microstructure. BCPB was not osteoinductive at all, but BCPA induced ectopic bone formation in 9 of 11 animals. Orthotopically, BCPA performed better than BCPB in both the amount and rate of bone formation. BCPC, similar to BCPA structurally and physicochemically, showed comparable results ectopically and orthotopically. Addition of resorbable polymer to BCPC made the material less osteoinductive (4 of 11 animals) and delayed bone formation orthotopically. Neither HA nor CA were osteoinductive, and their orthotopic performance was inferior to the osteoinductive ceramics. The results of the present study showed that material-derived osteoinduction significantly enhanced bone healing orthotopically, and that this material property appeared more sensitive for predicting orthotopic performance than physicochemical and structural characteristics. ß

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“…[15][16][17] BCPA and BCPB ceramics had similar chemical composition, crystallinity, and macrostructure, but their microstructure differed significantly. BCPA was highly microporous.…”

Section: Discussionmentioning

confidence: 99%

“…For implantation on the lumbar transverse processes, polymeric cassettes [15][16][17] were used. Each cassette housed two material conditions separated with a Teflon sheet.…”

Section: Animals and Implantationmentioning

confidence: 99%

Section: Animals and Implantationmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Comparative in vivo study of six hydroxyapatite‐based bone graft substitutes

Habibović

Kruyt

Juhl

et al. 2008

Journal Orthopaedic Research

Self Cite

214

186

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“…12 Therefore, the initial presence of viable cells inside clinically sized autografts may be relevant, not only because they participate in bone formation but also due to their stimulatory effect on physiologic remodeling of the graft. To investigate the hypothesis that viable cells enhance bone formation in autografts, we used our previously described transverse process model, 14,15 which focuses on the initial bone formation from the transverse process in posterolateral spinal fusion. 16 Viable and devitalized cancellous bone autografts were compared.…”

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confidence: 99%

Relevance of bone graft viability in a goat transverse process model

Kruyt

Delawi

Habibović

et al. 2008

Journal Orthopaedic Research

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Little is known about the mechanism by which autologous bone grafts are so successful. The relevance of viable osteogenic cells, which is a prominent difference between autologous bone graft and conventional alternatives, is especially controversial. With the emergence of bone tissue engineering, knowledge of the exact role of these cells has become crucial. The most obvious question to answer is whether viability of the graft has an effect on bone formation. In the current study, we investigated this effect of bone graft viability in a transverse process model that represents the initial bone formation in posterolateral spinal fusion. Eight goats received viable and devitalized autologous bone grafts in chambers mounted on the decorticated lumbar transverse processes. In addition, five goats received empty chambers. Histology and histomorphometry were performed after a 12-week implantation, and the dynamics of bone formation was monitored by sequential fluorochrome labeling. An obvious qualitative effect of viability was demonstrated by the presence of early onset osteogenesis distant from the transverse process bone in the viable grafts only. Quantitative analysis indicated about 30% more bone in the viable grafts, however, this difference was not statistically significant. In the empty chambers, bone was found in comparable quantities. We conclude that there is a qualitative advantage of graft viability in terms of early graft-derived osteogenesis. However, this advantage did not lead to significantly more bone formation in the viable bone grafts. ß

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Animal Models for Orthopedic Applications of Tissue Engineering

Viateau¹,

Decambron²

2014

Biomaterials

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The effect of cell-based bone tissue engineering in a goat transverse process model

Cited by 65 publications

References 28 publications

Comparative in vivo study of six hydroxyapatite‐based bone graft substitutes

Comparative in vivo study of six hydroxyapatite‐based bone graft substitutes

Relevance of bone graft viability in a goat transverse process model

Animal Models for Orthopedic Applications of Tissue Engineering

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