The effect of cerium oxide on lung injury following lower extremity ischemia-reperfusion injury in rats under desflurane anesthesia

Özdemirkan, Aycan; Küçük, Ayşegül; Güneş, İbrahim; Arslan, M; Tunçay, Aydın; Şıvgın, Volkan; Sezen, Şaban Cem; Boyunağa, Hakan

doi:10.15537/smj.2021.42.11.20210104

Cited by 8 publications

(10 citation statements)

References 14 publications

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“…They stated that serum MDA and NO levels were lower in the groups treated with CO and CO together with des urane compared with the IR group, and histopathologic results were better in these two groups. 34 In conclusion, CO administered intraperitoneally at a dose of 0.5 mg/kg 30 minutes before ischemia alone and in the des urane administered group reduced oxidative stress and corrected the liver damage caused by IRI. We think that CO administration before IRI and des urane anesthesia during IRI can have a liver protective effect in rats.…”

Section: Discussionmentioning

confidence: 79%

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Effects of cerium oxide on liver tissue in liver ischemia-reperfusion injury in rats undergoing desflurane anesthesia

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Küçük

Şengel

et al. 2022

Preprint

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Introduction During liver surgery and transplantation, periods of partial or total vascular occlusion are inevitable and result in ischemia-reperfusion injury (IRI). Nanomedicine uses the latest technology, which has emerged with interdisciplinary effects such as biomedical sciences, physics, and engineering, to protect and improve human health. Interdisciplinary research has brought along the introduction of antioxidant nanoparticles as potential therapeutics. This study’s goal was to investigate the effects of cerium oxide (CO) administration and desflurane anesthesia on liver tissue in liver IR injury. Material and Methods A total of 30 rats were randomly divided into five groups: control (C), ischemia-reperfusion (IR), IR-Desflurane (IRD), cerium oxide-ischemia reperfusion (CO-IR), cerium oxide-ischemia reperfusion-desflurane (CO-IRD). In the IR, IRD, and CO-IRD groups, hepatic ischemia was induced after the porta hepatis was clamped for 120 minutes, followed by 120 minutes of reperfusion. Intraperitoneal 0.5 mg/kg cerium oxide was administered to the cerium oxide groups 30 minutes before ischemia. Desflurane 6% was administered to the IRD and CO-IRD groups during IR. All groups were sacrificed under anesthesia. Liver tissue samples were examined under a light microscope by staining with hematoxylin-eosin (H&E). Malondialdehydes (MDA) levels, catalase (CAT), glutathione-s-transferase (GST), and arylesterase (ARE) enzyme activities were measured in tissue samples. Results The IR group had considerably more hydropic degeneration, sinusoidal dilatation, and parenchymal neutrophil infiltration than the IRD, CO-IR, and CO-IRD groups. CAT and GST enzyme activity was significantly higher in the CO-IR groups compared with the IR group. MDA levels were found to be significantly lower in the IRD, CO-IR, and CO-IRD groups compared with the IR group. Conclusion Intraperitoneal cerium oxide with desflurane reduced oxidative stress and corrected damage in the liver.

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Section: Discussionmentioning

confidence: 79%

“…We decided on the doses by taking previous studies as an example. 33,34 In our study, we evaluated oxidative stress and lipid peroxidation in liver tissue by measuring MDA levels, CAT, GST, and ARE enzyme activities.…”

Section: Discussionmentioning

confidence: 99%

Effects of cerium oxide on liver tissue in liver ischemia-reperfusion injury in rats undergoing desflurane anesthesia

Göbüt

Küçük

Şengel

et al. 2022

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“…Ozdemirkan et al investigated lung injury induced by CeO 2 and desflurane in rats with lower extremity IRI. They stated that serum MDA and nitric oxide levels were lower in the groups treated with CeO 2 , and CeO 2 together with desflurane, compared with the IR group, and histopathological results were better in these two groups [16].…”

Section: Discussionmentioning

confidence: 99%

“…Liver tissue samples were collected and kept in 10% formalin solution and serum samples at − 80 °C, and biochemical and histopathological tests were performed. The decision on the CeO 2 application dose and time was made based on the work done by Ozdemirkan et al [16].…”

Section: Experimental Groupsmentioning

confidence: 99%

Effects of cerium oxide (CeO2) on liver tissue in liver ischemia-reperfusion injury in rats undergoing desflurane anesthesia

Göbüt

Küçük²,

Şengel

et al. 2023

BMC Anesthesiol

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Introduction During liver surgery and transplantation, periods of partial or total vascular occlusion are inevitable and result in ischemia-reperfusion injury (IRI). Nanomedicine uses the latest technology, which has emerged with interdisciplinary effects, such as biomedical sciences, physics, and engineering, to protect and improve human health. Interdisciplinary research has brought along the introduction of antioxidant nanoparticles as potential therapeutics. The goal of this study was to investigate the effects of cerium oxide (CeO2) administration and desflurane anesthesia on liver tissue in liver IR injury. Material and methods Thirty rats were randomly divided into five groups: control (C), ischemia-reperfusion (IR), IR-desflurane (IRD), cerium oxide-ischemia reperfusion (CeO2-IR), and cerium oxide-ischemia reperfusion-desflurane (CeO2-IRD). In the IR, IRD, and CeO2-IRD groups, hepatic ischemia was induced after the porta hepatis was clamped for 120 min, followed by 120 min of reperfusion. Intraperitoneal 0.5 mg/kg CeO2 was administered to the CeO2 groups 30 min before ischemia. Desflurane (6%) was administered to the IRD and CeO2-IRD groups during IR. All groups were sacrificed under anesthesia. Liver tissue samples were examined under a light microscope by staining with hematoxylin-eosin (H&E). Malondialdehyde (MDA) levels, catalase (CAT), glutathione-s-transferase (GST), and arylesterase (ARE) enzyme activities were measured in the tissue samples. Results The IR group had considerably more hydropic degeneration, sinusoidal dilatation, and parenchymal mononuclear cell infiltration than the IRD, CeO2-IR, and CeO2-IRD groups. Catalase and GST enzyme activity were significantly higher in the CeO2-IR group than in the IR group. The MDA levels were found to be significantly lower in the IRD, CeO2-IR, and CeO2-IRD groups than in the IR group. Conclusion Intraperitoneal CeO2 with desflurane reduced oxidative stress and corrected liver damage.

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“… 10 In this experimental study, we preferred to intraperitoneally apply fullerenol C60 because it is easy to apply and provides rapid absorption. 44 , 45 …”

Section: Discussionmentioning

confidence: 99%

Effects of sevoflurane and fullerenol C60 on lower limb ischemia–reperfusion injury in streptozocin-induced diabetic mice

Polat,

Şengel,

Küçük

et al. 2024

Science Progress

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Background: Ischemia–reperfusion injury (IRI) poses a significant challenge for physicians, necessitating the management of cell damage and the preservation of organ functions. Various surgical procedures, such as vascular surgery on extremities, temporary cross-clamping of the abdominal aorta in aortic surgery, and the use of a tourniquet in extremity surgeries, may induce lower limb IRI. The susceptibility to IRI is heightened in individuals with diabetes. This study aimed to investigate the effects of fullerenol C60 and sevoflurane on mouse muscle tissue in a lower limb IRI model and to assess their potential in preventing complications arising from ischemia–reperfusion in mice with streptozocin-induced diabetes. Methods: A total of 36 adult Swiss albino mice were randomly divided into six groups, each consisting of six mice: control group (group C), diabetes group (group D), diabetes–ischemia/reperfusion group (group DIR), diabetes–ischemia/reperfusion–fullerenol C60 group (group DIR-FC60), diabetes–ischemia/reperfusion–sevoflurane group (group DIR-S), and diabetes–ischemia/reperfusion–sevoflurane–fullerenol C60 group (DIR-S-FC60). Streptozocin (55 mg/kg) was intraperitoneally administered to induce diabetes in the relevant groups, with mice displaying blood glucose levels of 250 mg/dL or higher at 72 h were considered diabetic. After 4 weeks, all groups underwent laparotomy under anesthesia. In DIR-FC60 and DIR-S-FC60 groups, fullerenol C60 (100 mg/kg) was intraperitoneally administrated 30 min before the ischemia period. Sevoflurane, delivered in 100% oxygen at a rate of 2.3% and 4 L/min, was administered during the ischemia period in DIR-S and DIR-S-FC60 groups. In the IR groups, a microvascular clamp was placed on the infrarenal abdominal aorta for 120 min during the ischemia period, followed by the removal of the clamp and a 120-min reperfusion period. At the end of the reperfusion, gastrocnemius muscle tissues were removed for histopathological and biochemical parameter examinations. Results: Histopathological examination revealed a significant reduction in the disorganization and degeneration of muscle cells in the DIR-S-FC60 group compared to the DIR group (p = 0.041). Inflammatory cell infiltration was notably lower in the DIR-S, DIR-FC60, and DIR-S-FC60 groups than in the DIR group (p = 0.031, p = 0.011, and p = 0.013, respectively). The total damage scores in the DIR-FC60 and DIR-S-FC60 groups were significantly lower than in the DIR group (p = 0.018 and p = 0.008, respectively). Furthermore, the levels of malondialdehyde (MDA) in the DIR-S, DIR-FC60, and DIR-S-FC60 groups were significantly lower than in the DIR group (p < 0.001, p < 0.001, and p < 0.001, respectively). Catalase (CAT) enzyme activity in the DIR-S, DIR-FC60, and DIR-S-FC60 groups was higher than in the DIR group (p = 0.001, p = 0.014, and p < 0.001, respectively). Superoxide dismutase (SOD) enzyme activity in the DIR-FC60 and DIR-S-FC60 groups was also higher than in the DIR group (p < 0.001 and p = 0.001, respectively). Conclusion: Our findings indicate that administering fullerenol C60 30 min prior to ischemia in diabetic mice, in combination with sevoflurane, led to a reduction in oxidative stress and the correction of IR-related damage in muscle tissue histopathology. We believe that the administration of fullerenol C60 before IR, coupled with sevoflurane administration during IR, exerts a protective effect in mice.

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The effect of cerium oxide on lung injury following lower extremity ischemia-reperfusion injury in rats under desflurane anesthesia

Cited by 8 publications

References 14 publications

Effects of cerium oxide on liver tissue in liver ischemia-reperfusion injury in rats undergoing desflurane anesthesia

Effects of cerium oxide on liver tissue in liver ischemia-reperfusion injury in rats undergoing desflurane anesthesia

Effects of cerium oxide (CeO2) on liver tissue in liver ischemia-reperfusion injury in rats undergoing desflurane anesthesia

Effects of sevoflurane and fullerenol C60 on lower limb ischemia–reperfusion injury in streptozocin-induced diabetic mice

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