The effect of charged groups on protein interactions with poly(HEMA) hydrogels

Effect of pH and Addition of Salt on the Adsorption Behavior of Lysozyme on Gold, Silica, and Titania Surfaces Observed by Quartz Crystal Microbalance with Dissipation Monitoring

“…The mode of binding has often been explained in terms of electrostatic interaction between the lysozyme and the charged surface 15,20,24) . Although the pH of saliva is usually buffered to neutral pH, a locally low pH could be caused by some bacterial acidic metabolites or due to a temporary decrease in pH by some food or beverage.…”

“…Such a modified methacrylic polymer surface could then be evaluated for the adsorption of lysozyme -again by the QCM-D method but using a sensor crystal with spin-coated polymer. This is a potential approach since the charged polymerlysozyme interaction has been successfully monitored by QCM-D for some anionic synthetic polymers 15,24) . As seen in Table 1, an adsorption of lysozyme as small as 10 ng/cm 2 (4×10 11 molecules/cm 2 ) was detected for the SiO2 sensor in the presence of 50 mM NaCl at pH 3.…”

“…This technique is sometimes used in combination with other methods such as AFM 15) or some optical techniques 14,16) . In the present study, the objective was to conduct a preliminary investigation to clarify the fundamental factors controlling the adsorption of lysozyme on some dental material-related surfaces, using the QCM-D method.…”

Charged hydrophilic polymer brushes and their relevance for understanding marine biofouling

“…Other cationic polymers containing derivatives of quaternary ammonium amine (Lee et al 2004) and imine (Yudovin-Farber et al 2010), or poly(vinyl-N-hexylpyridinium) (Tiller et al 2001) have also shown antibacterial activity in solution. In comparison to cationic polymers, there are far fewer studies on the effects of anionic polymers on biofouling and these are typically oriented toward biomedical materials (Lei et al 2014, Lord et al 2006). However, a recent study on the influence of charge of hydrogels immobilized onto polypropylene (PP) feed spacers for use in nanofiltration membrane systems has shown significant reduction in the attachment of Escherichia coli and delayed growth of biofilms on anionic hydrogel-coated PP, relative to that on neutral and zwitterionic coatings, after seven days of filtration (Wibisono et al 2015).…”

Antimicrobial Hydrogel Contact Lens Containing Alginate

“…Co-polymerization of 2-hydroxyethyl methacylate (HEMA) with monomers capable of establishing specific ionic or hydrophilic interactions with the drug molecules has been widely used to improve the release or loading behavior. [11][12][13] For instance, the hydrogels containing the comonomers methacrylic acid (MAA) or NVP (N-vinyl pyrrolidone) has been shown to be capable for a large amount of water. [14][15][16][17] The HEMA that has been copolymerized with anionic monomers such as MAA to introduce charged moieties in the polymeric network was found to improve the biological performance of the material.…”