The effect of chelating agents on the uptake and accumulation of cadmium by Mytilus edulis

George, Stephen; Coombs, Thomas L.

doi:10.1007/bf00391000

Cited by 181 publications

(53 citation statements)

References 11 publications

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“…Up to 100 ~g Cd 1-1, neither regulation nor limitation of uptake into mussel's soft body can be observed, indicating simple diffusion of Cd across the body surface. Uptake saturation processes, observed at about 1,500/~g Cd 1-1 (George & Coombs, 1977} and inhibition of Cd uptake by Ca ions as described by Wright (1977) for Carclnus maenas indicate at least a mediated Cd transport across cell membranes, but obviously can be neglected under slightly elevated Cd concentrations in sea water.…”

Section: Discussionmentioning

confidence: 86%

Accumulation, loss and molecular distribution of cadmium inMytilus edulis

Scholz

1980

Helgolander Meeresunters

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In Mytilus edulis, accumulation and loss of Cd were analyzed under experimental conditions. Cd uptake by the whole soft body is linear, increasing significantly with increasing Cd concentrations in the uptake medium. Until 100 #g Cd 1-1, neither limitation of uptake nor any saturation process can be observed. Loss of Cd, measured after transfer of experimentally contaminated mussels to natural sea water, is exponential; biological half lives vary between 14 and 29 days. Gills are the primary sites of Cd uptake from the water, whereas in mid-gut gland, kidney, and mantle the uptake is retarded during the first few days. The mid-gut gland not only bears the main body load of Cd, but also shows the highest Cd concentrations. Gel chromatographic studies of midgut gland proteins reveal that Cd is eluated over the whole molecular weight range. Three metallothionein-like proteins with molecular weights of 6,600, 13,200, and 21,000 Dalton could be established. However, they cannot be taken as effective detoxification proteins, because more than 50 % of the accumulated metal is bound to high molecular weight proteins.

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Section: Discussionmentioning

confidence: 86%

Accumulation, loss and molecular distribution of cadmium inMytilus edulis

Scholz

1980

Helgolander Meeresunters

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“…ion is the toxic species With the musse1 f·lytilus edulis, on the contrary, prior comp1exation of cadmium enhances its uptake (George &Coombs, 1977) and therefore probab1y its toxicity. Concerning the uptake of dissolved organics by nematodes, conflicting results exist in the literature.…”

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confidence: 99%

Toxicity of copper, mercury and lead to a marine nematode

Vranken

Heip

1986

Marine Pollution Bulletin

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Toxicity of copper, lead and mercury to the nematode Diplolaimella spec 1 is studied. Mortality responses obtained demonstrate high resistance to heavy metals. Population growth parameters as the intrinsic rate of natural increase and net-reproductivity are significantly depressed at copper-concentrations which cause no juve~ile mortality. The lowest concentrations tested caused significant inhibition of development rate in both sexes. For this particular nematode species suppression of fecundity and developmental inhibition are more reliable criteria, determining non-exceedable limits with regard to environmental safety. Our tests show that nematode productivity may be significant1y depressed at copper levels found in some areas of the North Sea. 173. 2. INTRODUCTI ONMarine nematodes are the most abundant anima1s in marine sediments and have severa1 features that are advantageous in ecotoxicological research in the laboratory : they have a short 1ife-span (Vranken & Heip, in press), a high fecundity (Vranken &Heip, 1983), represent several trophic levels (herbivores, bacteria1 feeders and carnivores) and at least some species are easi1y cultured (Heip et al., 1985).However, the effect of on1y a very limited set of chemical agents to on1ya few species has been tested up till now (Bogaert et a1., 1984; Howell, 1934; Vranken et al., 1934b). From these few experiments it appears that nematodes are relatively resistant to pollutants. This is corroborated by evidence from field surveys which have shown that nematode density is not affected substantia11y by raw domestic sewage (Vidakovic, 1983), heavy metals (Tietjen, 1977(Tietjen, , 1980 and acid iron waste (Lorenzen, 1974).In this paper we present results from tests on the species Dip101aimella spec I, a new species described by Jacobs &Vranken (in prep.) and misidentified as Monhystera microphthalma in previous papers.First, aseries of dcute (static) and sub1etha1 (chronic) tests on the toxicity of copper, mercury and lead is described, in which both mortality and deve10pment rate are studied simultaneous1y. Nematodes moult four times before becoming adult and the success in reaching a particular stage has been proposed as a sensitive indicator of toxicity (Samoiloff et a1., 1980). We used a slightly different approach and have determined generation time and preadult mortality as a function of metal concentration. These tests are presented as a supplement to the few data available and are intended to check whether the resistance of nematodes to toxicants is indeed true. Second, we will also report on sublethal effects of copper on fecundity, because mortality is not a 174 • very sensitive index of toxicity. Fecundity, the number of eggs produced, has been used because it is known to be a sensitive criterion in toxicity testing (Bryan, 1980;Reish &Carr, 1978).As a combination of fecundity and deyelop~ent rate (generation time) the intrinsic rate of natural increase r m of the population can be calculated. This has been done by Tietjen &Lee (1984) to measur...

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“…For example, in gill tissue low levels of accumulated Cd can cause an increase of MTs , while in the digestive gland of different bivalve species Cd accumulation to a certain extent does not cause corresponding increase of MT level (Bebianno et al, 1993;Geffard et al, 2001;Raspor et al, 2004). Furthermore, the lower concentrations of trace metals were reported for gonadic tissue in comparison with the other mussel tissues (George and Coombs, 1977;La Touche and Mix, 1982;Lobel and Wright, 1982). The combined effect of different biochemical responses in different tissues leads to weaker relationship (R 2 = 0.23, p<0.05) between MTs and Cd in the whole soft tissue.…”

Section: Principal Component Analysismentioning

confidence: 95%

The Influence of the Biometric Parameters on Metallothionein and Metal Level in the Heat-Treated Cytosol of the Whole Soft Tissue of Transplanted Mussels

Dragun

Raspor

Erk

et al. 2006

Environ Monit Assess

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The influence of the biometric parameters (shell mass, whole soft tissue mass, condition index) on MT and metal levels in the heat-treated cytosol of the whole soft tissue of transplanted mussels was studied over the period of one year. The positive correlation of three metals (Cd, Fe, Zn) with the shell mass indicated to time-dependent increase of their contents.Strong correlation of Mn content with the whole soft tissue mass (r = 0.74, p<0.0001), and almost identical changes of these two parameters over the year make Mn a good indicator of mussel's condition. As opposed to the other metals, Cu content does not exhibit connection with biometry. On the other hand, MTs are highly influenced by biometry. As much as 65% of their variability could be explained by the changes of the shell mass and the whole soft tissue mass. Consequently, it is difficult to distinguish if the obtained positive correlation between Cd and MTs (r=0.48, p<0.05) reflects MT induction by Cd, or Cd accumulation as a result of age-dependent increase of MTs. Due to the strong influence of the biometry on MT level, the whole soft tissue is not considered as the best choice for measuring MTs as a biomarker. Better option would be to isolate a specific tissue that shows indisputable connection between MT induction and metal accumulation.

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The effect of chelating agents on the uptake and accumulation of cadmium by Mytilus edulis

Cited by 181 publications

References 11 publications

Accumulation, loss and molecular distribution of cadmium inMytilus edulis

Accumulation, loss and molecular distribution of cadmium inMytilus edulis

Toxicity of copper, mercury and lead to a marine nematode

The Influence of the Biometric Parameters on Metallothionein and Metal Level in the Heat-Treated Cytosol of the Whole Soft Tissue of Transplanted Mussels

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