The effect of chilling on membrane lipid phase transition in human oocytes and zygotes

Ghetler, Yehudith; Yavin, S.; Shalgi, Ruth; Arav, Amir

doi:10.1093/humrep/dei236

Cited by 150 publications

(81 citation statements)

References 41 publications

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“…Undoubtedly, the permeability of immature oocytes to cryoprotectant agents depends not only on their membrane lipid composition [51] and cortical microfilament network [52], but also on the presence of a somatic vestment. However, studies on this subject are not consistent.…”

Section: Oocyte-cumulus Cell Contact and Interactionmentioning

confidence: 99%

“…It is reported that the oolemma of GV-stage oocytes contains high aquaporin [51]. In the plasma membrane of mature oocytes there are elevated concentration of saturated fatty acids (79,22 %) and a content of polyunsaturated fatty acids of 6,5 % [61].…”

Section: Oolemmamentioning

confidence: 99%

“…In the plasma membrane of mature oocytes there are elevated concentration of saturated fatty acids (79,22 %) and a content of polyunsaturated fatty acids of 6,5 % [61]. This particular composition of lipids makes the mature oocytes membrane more fluid and so more resistant at low temperature in comparison with immature stages, decreasing their sensitivity to chilling injury [51,[62][63][64].…”

Section: Oolemmamentioning

confidence: 99%

“…On the basis that a low lipid phase transition temperature is associated with chilling resistance [66], Ghetler et al investigated the difference of Tm between human zygotes, in-vitro matured and immature GV oocytes by means of a Fourier Transform Infrared spectroscopy [51]. The Tm of human zygotes is about 10°C and far below room temperature, thermal condition at which the process of dehydration takes place.…”

Section: Oolemmamentioning

confidence: 99%

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The current challenges to efficient immature oocyte cryopreservation

Brambillasca

Guglielmo

Coticchio

et al. 2013

J Assist Reprod Genet

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Oocyte cryopreservation represents an important tool for assisted reproductive technology. It offers the opportunity to preserve fertility in women at risk of loss of the ovarian function for various pathologies. It also represents a treatment alternative for couples that cannot benefit from embryo cryopreservation because of moral, religious, or legal constrains. On the other hand, in vitro oocyte maturation has a range of applications. It can be applied in patients with a contraindication to ovarian stimulation to prevent ovarian hyperstimulation syndrome or to eliminate the risk of stimulation of hormone-sensitive tumours in cancer patients. However, while mature oocyte cryopreservation has found widespread application and oocyte in vitro maturation has a place for the treatment of specific clinical conditions, data on the efficiency of freezing of immature or in vitro matured oocytes are poorer. In this review we will focus on the combination of oocyte in vitro maturation with oocyte cryopreservation with particular emphasis on the biological implications of the cryopreservation of immature or in vitro matured oocytes. The two cryopreservation approaches, slow freezing and vitrification, will be discussed in relation to possible cryodamage occurring to subcellular structures of the oocyte and the functional interaction between oocyte and cumulus cells.

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Section: Oocyte-cumulus Cell Contact and Interactionmentioning

confidence: 99%

Section: Oolemmamentioning

confidence: 99%

Section: Oolemmamentioning

confidence: 99%

Section: Oolemmamentioning

confidence: 99%

See 2 more Smart Citations

The current challenges to efficient immature oocyte cryopreservation

Brambillasca

Guglielmo

Coticchio

et al. 2013

J Assist Reprod Genet

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“…With the exception of an analysis of Van Blerkom and Davies published in the mid-nineties [21], further evidence on the ultrastructure of frozen-thawed oocytes has been gained only recently. Ultrastructural alterations involving the cortical granules (CG), ZP, organelle associations, and ooplasm were reported in coincidence with the application of particular dehydration and rehydration conditions [19,[22][23][24] or possibly specific CPAs [23], suggesting that TEM can represent a first line investigative tool.…”

Section: Introductionmentioning

confidence: 99%

Qualitative and morphometric analysis of the ultrastructure of human oocytes cryopreserved by two alternative slow cooling protocols

Coticchio¹,

Borini²,

Distratis³

et al. 2010

J Assist Reprod Genet

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Purpose To ascertain possible cell damage from cryopreservation, the ultrastructure of human oocytes cryopreserved by slow cooling was assessed. Materials and methods Cryopreservation was performed through two protocols with one-step or two-step propanediol. Fresh control oocytes were examined for comparison. Samples were processed for transmission electron microscopy analysis. Results By light microscopy, both fresh and frozen-thawed oocytes appeared regularly rounded, with intact zona pellucida, and homogeneous cytoplasm. By electron microscopy observation, organelles were abundant and uniformly dispersed. Mitochondria-smooth endoplasmic reticulum associations appeared regular. However, both the amount and density of cortical granules appeared abnormally reduced in frozen-thawed samples. Slight to moderate vacuolization was also found in the ooplasm of oocytes of both frozen groups. Conclusions Slow cooling ensures a good overall preservation of human oocytes. However, cytoplasmic vacuolization and cortical granule loss appears associated with cryopreservation, irrespective of the protocol used.

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Novel Imaging Techniques to Assess Gametes and Preimplantation Embryos

Swain

2016

Human Reproduction

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The effect of chilling on membrane lipid phase transition in human oocytes and zygotes

Cited by 150 publications

References 41 publications

The current challenges to efficient immature oocyte cryopreservation

The current challenges to efficient immature oocyte cryopreservation

Qualitative and morphometric analysis of the ultrastructure of human oocytes cryopreserved by two alternative slow cooling protocols

Novel Imaging Techniques to Assess Gametes and Preimplantation Embryos

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