The effect of chitosan hydrogel containing DMEM/F12 medium on full-thickness skin defects after deep dermal burn

Kiyozumi, Tetsuro; Kanatani, Yasuhiro; Ishihara, Masayuki; Saitoh, Daizoh; Shimizu, Jun; Yura, Hirofumi; Suzuki, Shinya; Okada, Yoshiaki; Kikuchi, Makoto

doi:10.1016/j.burns.2006.09.010

Cited by 48 publications

(39 citation statements)

References 24 publications

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“…An ideal gel formulation used for burn healing should be easily applicable and retained for a long time on the wound. 21,22) Therefore, a gel should show appropriate viscosity. In our study, the viscosity of the fucoidan-chitosan hydrogels which affected by molecular weight of chitosan and concentration of polymer (pϽ0.05) were ranged between 801 and 22.338 cPs (Table 1) and obtained results were consistent with the literature.…”

Section: Resultsmentioning

confidence: 99%

Preparation of Fucoidan-Chitosan Hydrogel and Its Application as Burn Healing Accelerator on Rabbits

Sezer

Cevher

Hatıpoğlu

et al. 2008

Biological & Pharmaceutical Bulletin

161

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Burn healing includes a specific biological process related to the general phenomenon of growth and regeneration and the primary objective in burn care is the promotion of rapid wound healing with the best functional results. The principal function of a burn dressing is to provide an optimum healing milieu for natural healing and desirable burn dressing may, therefore, be characterized on the basis of its performance such as; (a) provision of adequate gaseous exchange, (b) provision and maintenance of a moist environment, (c) protection from infections and contamination, (d) absorption of wound fluids and (e) painless and easy removal. 1,2) Hydrogels are ideal biopolymeric pharmaceutical forms for the treatment of skin wounds. They have low interfacial tension, high molecular and oxygen permeability, good moisturizing and mechanical properties that resemble physiological soft tissue. 3,4) For this reasons, polysaccharides, e.g. chitosan, are having hydrogel forming properties have been considered to be advantageous in its application as a wound dressing material. 5) Chitosan, [a (1→4) 2-amino-2-deoxy-b-D-glucan], a unique polysaccharide derived from deacetylation of chitin, has been used in wound treatment owing to its good biocompatibility, biodegradability and accelerated granulation. [6][7][8] On the other hand, fucoidan is a sulphated polyfucose polysaccharide and has attracted considerable biotechnological research interest since the discovery that it possessed anti-coagulant activity similar to that of heparin and also reported to possess other properties including antithrombotic, anti-inflammatory, anti-tumoral and anti-viral effects. 9,10) Many of these effects are thought to be due to its interaction with growth factors such as basic fibroblast growth factor (bFGF) and transforming growth factor-b (TGF-b). Fucoidan may, therefore, be able to modulate growth factordependent pathways in the cell biology of tissue repair. 11) Although a great number of studies on different pharmacological properties of fucoidan and chitosan are present, there is little information on the fucoidan-based system used in burn healing and its only limited with cell culture. 12) The aim of this study was to prepare fucoidan-chitosan hydrogel and to investigate its treatment efficiency on dermal burns on rabbits. MATERIALS AND METHODSMaterials Chitosan (MW 250 kDa, deacetylation degree Ն90%, Pronova A/S, Norway; MW 400 kDa, deacetylation degree Ն60%, Fluka, Germany; MW 750 kDa, deacetylation degree Ն75%, Sigma, U.S.A.), Fucoidan (from Fucus vesiculosus) and lactic acid (85% m/v) purchased from Sigma, U.S.A. All other reagents used were of analytical grade.Preparation of Hydrogels The composition of gel formulations was given in Table 1. Fucoidan was dissolved in 1% m/v lactic acid solution by mechanical shaking at 300 rpm for 1 h. Then chitosan was left to swell in the fucoidan solution overnight to prepare hydrogels. 13) Formulations were kept at 4°C until experiment.Viscosity Measurement The viscosity of hydrogels was determine...

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Section: Resultsmentioning

confidence: 99%

Preparation of Fucoidan-Chitosan Hydrogel and Its Application as Burn Healing Accelerator on Rabbits

Sezer

Cevher

Hatıpoğlu

et al. 2008

Biological & Pharmaceutical Bulletin

161

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“…Tissue engineering has resulted in the gener- ation of various scaffolds to treat excisional wounds and promote wound healing. Chitosan has shown promise in the form of chitosan hydrogels and chitosan acetate bandages, as well as chitosan-(polyvinyl alcohol) nanofibers (50)(51)(52). Although the main purpose of this study was to determine if NF wound dressings could treat S. aureus-induced infections, the effects of the NF and CF wound dressings on wound closure were also investigated.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a Nisin-Eluting Nanofiber Scaffold To Treat Staphylococcus aureus-Induced Skin Infections in Mice

Heunis

Smith

Dicks

2013

Antimicrob Agents Chemother

130

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Staphylococcus aureus is a virulent pathogen and a major causative agent of superficial and invasive skin and soft tissue infections (SSSTIs). Antibiotic resistance in S. aureus, among other bacterial pathogens, has rapidly increased, and this is placing an enormous burden on the health care sector and has serious implications for infected individuals, especially immunocompromised patients. Alternative treatments thus need to be explored to continue to successfully treat infections caused by S. aureus, including antibiotic-resistant strains of S. aureus. In this study, an antimicrobial nanofiber wound dressing was generated by electrospinning nisin (Nisaplin) into poly(ethylene oxide) and poly(D,L-lactide) (50:50) blend nanofibers. Active nisin diffused from the nanofiber wound dressings for at least 4 days in vitro, as shown by consecutive transfers onto plates seeded with strains of methicillin-resistant S. aureus (MRSA). The nisin-containing nanofiber wound dressings significantly reduced S. aureus Xen 36 bioluminescence in vivo and viable cell numbers in a murine excisional skin infection model. The bacterial burden of wounds treated with nisin-containing nanofiber wound dressings was 4.3 ؋ 10 2 CFU/wound, whereas wounds treated with control nanofiber wound dressings had 2.2 ؋ 10 7 CFU/wound on the last day of the trial (day 7). Furthermore, the wound dressings stimulated wound closure of excisional wounds, and no adverse effects were observed by histological analysis. Nisin-containing nanofiber wound dressings have the potential to treat S. aureus skin infections and to potentially accelerate wound healing of excisional wounds.

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“…Hydrogels, structurally similar to the natural extracellular matrix, can be designed to provide instructive environments for the three-dimensional assembly of vascular networks. Many studies of hydrogel-based scaffolds have focused on applications in healing wounds (5,(16)(17)(18)(19)(20)(21). Beyond their utility as scaffolds, hydrogels can also deliver cytokines and growth factors (22,23), antibiotics (20), and cells (24,25) to allow complete skin regeneration.…”

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confidence: 99%

Dextran hydrogel scaffolds enhance angiogenic responses and promote complete skin regeneration during burn wound healing

Sun

Zhang

Shen

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

477

307

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Neovascularization is a critical determinant of wound-healing outcomes for deep burn injuries. We hypothesize that dextran-based hydrogels can serve as instructive scaffolds to promote neovascularization and skin regeneration in third-degree burn wounds. Dextran hydrogels are soft and pliable, offering opportunities to improve the management of burn wound treatment. We first developed a procedure to treat burn wounds on mice with dextran hydrogels. In this procedure, we followed clinical practice of wound excision to remove full-thickness burned skin, and then covered the wound with the dextran hydrogel and a dressing layer. Our procedure allows the hydrogel to remain intact and securely in place during the entire healing period, thus offering opportunities to simplify the management of burn wound treatment. A 3-week comparative study indicated that dextran hydrogel promoted dermal regeneration with complete skin appendages. The hydrogel scaffold facilitated early inflammatory cell infiltration that led to its rapid degradation, promoting the infiltration of angiogenic cells into the healing wounds. Endothelial cells homed into the hydrogel scaffolds to enable neovascularization by day 7, resulting in an increased blood flow significantly greater than treated and untreated controls. By day 21, burn wounds treated with hydrogel developed a mature epithelial structure with hair follicles and sebaceous glands. After 5 weeks of treatment, the hydrogel scaffolds promoted new hair growth and epidermal morphology and thickness similar to normal mouse skin. Collectively, our evidence shows that customized dextran-based hydrogel alone, with no additional growth factors, cytokines, or cells, promoted remarkable neovascularization and skin regeneration and may lead to novel treatments for dermal wounds.

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The effect of chitosan hydrogel containing DMEM/F12 medium on full-thickness skin defects after deep dermal burn

Cited by 48 publications

References 24 publications

Preparation of Fucoidan-Chitosan Hydrogel and Its Application as Burn Healing Accelerator on Rabbits

Preparation of Fucoidan-Chitosan Hydrogel and Its Application as Burn Healing Accelerator on Rabbits

Evaluation of a Nisin-Eluting Nanofiber Scaffold To Treat Staphylococcus aureus-Induced Skin Infections in Mice

Dextran hydrogel scaffolds enhance angiogenic responses and promote complete skin regeneration during burn wound healing

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