The effect of chondroitin sulfate concentration and matrix stiffness on chondrogenic differentiation of mesenchymal stem cells

Ai, Chengchong; Liu, Ling; Wong, Kim Ping; Tan, Xuan Hao; Goh, James Cho Hong

doi:10.1039/d2bm01980a

Cited by 8 publications

(4 citation statements)

References 60 publications

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“…A similar study on BMSCs pointed out that stiffness varying can form a suitable network for cartilage differentiation of BMSCs 57 . There is evidence that ADSCs are more likely to differentiate into cartilage than BMSCs; therefore, optimization of hydrogel matrix stiffness favors for MSC chondrogenic differentiation was practicable 58 . Considering decorin was necessary for maintaining fibril structure and mechanical stiffness of tendon and cartilage 59 , 60 , we assumed that application of decorin in gellan gum could serve as viscous substrates to modulate stiffness in a microenvironment, which preserves ADSCs regenerative potential under inflammation.…”

Section: Discussionmentioning

confidence: 99%

Injectable Decorin/Gellan Gum Hydrogel Encapsulating Adipose-Derived Stem Cells Enhances Anti-Inflammatory Effect in Cartilage Injury via Autophagy Signaling

He,

Wu,

Luo

et al. 2023

Cell Transplant

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Adipose-derived stem cells (ADSCs) are employed as a promising alternative in treating cartilage injury. Regulating the inflammatory “fingerprint” of ADSCs to improve their anti-inflammatory properties could enhance therapy efficiency. Herein, a novel injectable decorin/gellan gum hydrogel combined with ADSCs encapsulation for arthritis cartilage treatment is proposed. Decorin/gellan gum hydrogel was prepared according to the previous manufacturing protocol. The liquid–solid form transition of gellan gum hydrogel is perfectly suitable for intra-articular injection. Decorin-enriched matrix showing an immunomodulatory ability to enhance ADSCs anti-inflammatory phenotype under inflammation microenvironment by regulating autophagy signaling. This decorin/gellan gum/ADSCs hydrogel efficiently reverses interleukin-1β-induced cellular injury in chondrocytes. Through a mono-iodoacetate-induced arthritis mice model, the synergistic therapeutic effect of this ADSCs-loaded hydrogel, including inflammation attenuation and cartilage protection, is demonstrated. These results make the decorin/gellan gum hydrogel laden with ADSCs an ideal candidate for treating inflammatory joint disorders.

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Section: Discussionmentioning

confidence: 99%

Injectable Decorin/Gellan Gum Hydrogel Encapsulating Adipose-Derived Stem Cells Enhances Anti-Inflammatory Effect in Cartilage Injury via Autophagy Signaling

He,

Wu,

Luo

et al. 2023

Cell Transplant

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“…However, ChSMA has also been reported to have an effect. A ChSMA concentration of 6 % was found to be best at promoting chondrogenic differentiation of BMSCs, mainly because such a density is optimal for cell interaction [ 26 ]. Similarly, ChSMA increased Col2a1, Sox9, and Aca by 5.9, 10.97, and 5.56 times on day 14 compared to the control group.…”

Section: Resultsmentioning

confidence: 99%

“…Thus, they are often used as scaffolds with RGD peptide for tissue engineering [ 25 ]. Chondroitin Sulfate (ChS) is a natural glycosaminoglycan (≈500 Da), which is an important structural component of cartilage and other connective tissues, with many positive properties, such as promoting chondrogenic differentiation of BMSCs [ 26 ], fostering cartilage generation by balancing chondrocyte metabolism [ 27 , 28 ], promoting anti-inflammation [ 29 ], inhibiting cellular hypertrophy [ 30 ] and enhancing integration with natural cartilage [ 31 ]. However, ChS cannot be used alone to prepare scaffolds because of its good water solubility and inability to form a hydrogel by self-assembly.…”

Section: Introductionmentioning

confidence: 99%

Cell-recruited microspheres for OA treatment by dual-modulating inflammatory and chondrocyte metabolism

Zhou,

He,

Zhang

et al. 2024

Materials Today Bio

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“…On the other hand, it is worth noting that signs of hypertrophic chondrogenic differentiation are commonly observed in cartilage tissue engineering research. For instance, a recent study showed that MSCs cultured in hydrogels composed of gelatin methacryloyl (GelMA) and different concentrations of methacrylated chondroitin sulfate (CSMA) increased COL10 expression across all concentrations [ 39 ]. These findings highlight the challenge of hypertrophic differentiation and emphasize the need to investigate strategies aimed at minimizing or inhibiting this process.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a Peptide Hydrogel as a Chondro-Instructive Three-Dimensional Microenvironment

Nogoceke,

Josino,

Robert

et al. 2023

Polymers

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Articular cartilage injuries are inherently irreversible, even with the advancement in current therapeutic options. Alternative approaches, such as the use of mesenchymal stem/stromal cells (MSCs) and tissue engineering techniques, have gained prominence. MSCs represent an ideal source of cells due to their low immunogenicity, paracrine activity, and ability to differentiate. Among biomaterials, self-assembling peptide hydrogels (SAPH) are interesting given their characteristics such as good biocompatibility and tunable properties. Herein we associate human adipose-derived stem cells (hASCs) with a commercial SAPH, Puramatrix™, to evaluate how this three-dimensional microenvironment affects cell behavior and its ability to undergo chondrogenic differentiation. We demonstrate that the Puramatrix™ hydrogel comprises a highly porous matrix permissible for hASC adhesion and in vitro expansion. The morphology and cell growth dynamics of hASCs were affected when cultured on the hydrogel but had minimal alteration in their immunophenotype. Interestingly, hASCs spontaneously formed cell aggregates throughout culturing. Analysis of glycosaminoglycan production and gene expression revealed a noteworthy and donor-dependent trend suggesting that Puramatrix™ hydrogel may have a natural capacity to support the chondrogenic differentiation of hASCs. Altogether, the results provide a more comprehensive understanding of the potential applications and limitations of the Puramatrix™ hydrogel in developing functional cartilage tissue constructs.

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The effect of chondroitin sulfate concentration and matrix stiffness on chondrogenic differentiation of mesenchymal stem cells

Abstract: The effect of matrix stiffness and chondroitin sulfate concentration on stem cell chondrogenesis was investigated.

Cited by 8 publications

References 60 publications

Injectable Decorin/Gellan Gum Hydrogel Encapsulating Adipose-Derived Stem Cells Enhances Anti-Inflammatory Effect in Cartilage Injury via Autophagy Signaling

Injectable Decorin/Gellan Gum Hydrogel Encapsulating Adipose-Derived Stem Cells Enhances Anti-Inflammatory Effect in Cartilage Injury via Autophagy Signaling

Cell-recruited microspheres for OA treatment by dual-modulating inflammatory and chondrocyte metabolism

Evaluation of a Peptide Hydrogel as a Chondro-Instructive Three-Dimensional Microenvironment

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