The effect of chromatographic conditions on the separation of selected alkaloids on CN-silica layers

Advances of Ionic Liquids in Analytical Chemistry

“…The separation was achieved in only 25 min, and the HPTLC was coupled with MS to increase the sensitivity of the method. In another study, mobile phases containing ILs were used in two-dimensional TLC (2D-TLC) for the separation of alkaloids and its identification in plant extracts . The separations were performed on glass cyanopropyl plates using a mixture of methanol, diisopropyl ether, ammonia, and acetic acid in the first dimension and acetonitrile, water, formic acid, and 1-butyl-3-methylimidazolium tetrafluoroborate ([C 4 MIM + ]­[BF 4 – ]) in the second dimension.…”