The Effect of Ciprofloxacin and Gentamicin on Spinal Morphine-Induced Antinociception in Rats*

Abstract

This paper investigates the possible antinociceptive effect of systemically administered ciprofloxacin and gentamicin and its influence on intrathecal morphine-induced antinociception. Using thermal nociceptive tests (hot-plate test and tail-flick test) and a motor function test (catalepsy test) in male Sprague-Dawley rats (nΩ5-9/dose), the following observations were made: ciprofloxacin administered intraperitoneally in the dose range 4-64 mg/kg demonstrated a modest antinociceptive effect in both nociceptive tests. Solvent of ciprofloxacin (intraperitoneally) and saline (intraperitoneally), given as a control, showed no effect. Gentamicin, administered at a dose of 0. . These data show that intraperitoneal administration of ciprofloxacin and gentamicin produces a modest antinociceptive effect in the hotplate test and tail-flick test. Ciprofloxacin, but not gentamicin, can interact at least additively to increased naloxonereversible morphine intrathecal antinociception. Differences in the ability to penetrate the blood-brain barrier between the two antibiotics could explain the lack of effect from gentamicin in the hot plate and on morphine-induced antinociception.

The Effect of Tempol Administration on the Aortic Contractile Responses in Rat Preeclampsia Model

“…A similar result has been reported by Preti et al [17]. As stated above, tempol changes the highly reactive O 2 − to H 2 O 2 .…”

Reply

“…We strictly reject the accusation that the work by Luger et al . (2005), reported unethical actions and “raised concern about appropriate animal welfare, protection of animals and humane endpoints”.…”

“…Thank you very much for the opportunity to reply to the abovementioned letter. We were both surprised and amazed that the letter by Svendsen summarily criticizes two non‐comparable articles; our reply will therefore concentrate only on the remarks concerning the work by Luger et al . (2005), and not on those dealing with the work by Muriel et al .…”

“…A quick search in Pubmed produced 14,035 hits (2541 since the year 2000) for the term “rat intraperitoneal administration.” Were all those studies unethical, and did they raise concerns about inappropriate animal welfare? Moreover, the study by Luger et al . (2005), was approved by the Austrian Federal Animal Investigation Committee (Austrian Animal Protection Act, Federal Law Gazette No.…”

Ethics and Animal Welfare Related to in vivo Pharmacology and Toxicology in Laboratory Animals

“…In the paper by Luger et al (2005) the formulations of antibiotics were administered intraperitoneally. The dose volume was 2 ml, which was administered over 20 sec.…”