The effect of ciprofloxacin on the growth of B16F10 melanoma cells

Jaber, Dalal F.; Jallad, Mary-Ann; Abdelnoor, Alexander Micheal

doi:10.4103/0973-1482.180610

Cited by 9 publications

(5 citation statements)

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“…Furthermore, the present analysis revealed that the expression of CDCA8 was significantly associated with lymph node metastasis. Regional lymph node metastases constitute the most common mode of initial presentation with metastatic melanoma ( 22 - 24 ). The updated 2009 AJCC melanoma staging system reported that, in the absence of nodal metastases, patients with intralymphatic metastases have a 5-year survival rate of only 69% ( 25 ), which may be one of the reasons CDCA8 affects the prognosis of cutaneous melanoma patients.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of CDCA8 promotes the malignant progression of cutaneous melanoma and leads to poor prognosis

Tang

Lyu

et al. 2018

Int J Mol Med

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Cutaneous melanoma is very aggressive and results in high mortality rates for cancer patients. Determining molecular targets is important for developing novel therapies for cutaneous melanoma. Cell division cycle associated 8 (CDCA8) is a putative oncogene that is upregulated in multiple types of cancer. The present study aimed to examine the role of CDCA8 in cutaneous melanoma, with a focus on the association of its expression to prognosis and metastasis. First, the mRNA expression of CDCA8 in cutaneous melanoma tissues was investigated using the ONCOMINE and Gene Expression Omnibus (GEO) databases. Furthermore, the relationship between the expression of CDCA8 and cutaneous melanoma patient survival was analyzed using a Kaplan-Meier plot and Log Rank test. In addition, the effects of CDCA8 on proliferation, migration and invasion of cutaneous melanoma cell lines were investigated using reverse transcription-quantitative polymerase chain reaction (RT-qPCR), Cell Counting kit-8, colony formation assay, wound healing and Matrigel assay. Finally, the expression levels of key proteins related to the Rho-associated coiled-coil-containing protein kinase (ROCK) signaling pathway were measured by western blot assay. The results demonstrated that CDCA8 was overexpressed in cutaneous melanoma tissues and cells lines compared with normal tissues, and high expression of CDCA8 was significantly associated with poorer prognosis in patients with cutaneous melanoma. In in vitro experiments, CDCA8 knockdown inhibited A375 and MV3 cell proliferation, migration and invasion. In addition, CDCA8 knockdown reduced the phosphorylation levels of ROCK1 and myosin light chain, two downstream effector proteins of the ROCK pathway. In summary, the present findings suggested that CDCA8 may be a promising therapeutic target for the treatment of cutaneous melanoma.

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Section: Discussionmentioning

confidence: 99%

Overexpression of CDCA8 promotes the malignant progression of cutaneous melanoma and leads to poor prognosis

Tang

Lyu

et al. 2018

Int J Mol Med

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“…Ciprofloxacin has been extensively studied for its anticancer effects both in vitro and in vivo. Studies have shown that ciprofloxacin inhibits the cellular proliferation of melanoma cells B16F10, triple-negative breast cancer cells MDA-MB−231, pancreatic cancer cells Panc−1, non-small cell lung cancer cells A549, and hepatocellular carcinoma cells HepG2 [28,29,30,31]. Here, we report the chemosensitizing effects of ciprofloxacin by inhibiting the efflux function of ABCB1 transporter.…”

Section: Discussionmentioning

confidence: 74%

Ciprofloxacin Enhances the Chemosensitivity of Cancer Cells to ABCB1 Substrates

Gupta

Gao

Ashar

et al. 2019

IJMS

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ABCB1 is one of the major drug efflux transporters that is known to cause multidrug resistance (MDR) in cancer patients receiving chemotherapy for the treatment of solid tumors and hematological malignancies. Inhibition of ABCB1 efflux function is important for maintaining the intracellular concentration of chemotherapeutic drugs. Here, we evaluated ciprofloxacin for its ability to reverse MDR caused by the overexpression of ABCB1. Cytotoxicity of ciprofloxacin was determined by the MTT assay. The chemosensitizing effects of ciprofloxacin were determined in combination with ABCB1 substrates. The intracellular accumulation and efflux of ABCB1 substrates was measured by a scintillation counter, and protein expression was determined by the Western blotting. Vanadate-sensitive ATPase assay was performed to determine the effect of ciprofloxacin on the ATPase activity of ABCB1, and docking analysis was done to determine the interaction of ciprofloxacin with ABCB1. Ciprofloxacin significantly potentiated the cytotoxic effects of ABCB1 substrates in ABCB1-overexpressing cells. Furthermore, ciprofloxacin increased the intracellular accumulation and decreased the efflux of [3H]-paclitaxel without altering the expression of ABCB1. Ciprofloxacin stimulated the ATPase activity of ABCB1 in a concentration-dependent manner. Our findings showed that ciprofloxacin potently inhibits the ABCB1 efflux function and it has potential to be developed as a combination anticancer therapy.

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“…In comparison, the enzyme concentration in tumor cells is higher [10]. Several previous studies have already proven the efficacy of CPX in inhibiting hTOPO-II, leading to the anti-proliferative and cytotoxic activities of this molecule against several malignant cells [74,108]. Accordingly, we can expect that the CPX probe will be efficient and able to reach the desired location.…”

Section: Spectroscopic Parameters: 19 F-chemical Shifts (δ)mentioning

confidence: 95%

Structural Parameters of the Interaction between Ciprofloxacin and Human Topoisomerase-II β Enzyme: Toward New 19F NMR Chemical Shift Probes

2022

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New tools for cancer diagnosis are being studied since early diagnosis can be crucial for a successful treatment. In this context, the use of NMR probes constitutes an efficient method of diagnosis. In this study, we investigated the use of ciprofloxacin to indirectly label the overexpression of topoisomerase-II enzymes by changes in 19F NMR chemical shifts of ciprofloxacin. Increased topoisomerase-II expression has been associated with cancer occurrence, mainly with aggressive forms of breast cancer, thus constituting a promising molecular target for new tumor cell identifiers. Using DFT calculations, we performed a spectroscopy analysis of ciprofloxacin in different chemical environments and evaluated the solvent and enzymatic effects. Our results show that ciprofloxacin forms a stable complex with the enzyme, and the main intermolecular interactions between ciprofloxacin and human topoisomerase-IIβ are hydrogen bonds, followed by π-π stacking and electrostatic interactions. Additionally, a shift of 6.04 ppm occurs in the 19F NMR signal when ciprofloxacin interacts with the human topoisomerase-IIβ enzyme, and this parameter may be an indirect marker indicating the overexpression of these enzymes in the body.

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The effect of ciprofloxacin on the growth of B16F10 melanoma cells

Abstract: Ciprofloxacin seems to exhibit antitumor activity both in-vivo and in-vitro. This effect might be explained by several mechanisms such as directly inducing cancer cell death or altering the immune response through the modification of the normal microbiota.

Cited by 9 publications

References 0 publications

Overexpression of CDCA8 promotes the malignant progression of cutaneous melanoma and leads to poor prognosis

Overexpression of CDCA8 promotes the malignant progression of cutaneous melanoma and leads to poor prognosis

Ciprofloxacin Enhances the Chemosensitivity of Cancer Cells to ABCB1 Substrates

Structural Parameters of the Interaction between Ciprofloxacin and Human Topoisomerase-II β Enzyme: Toward New 19F NMR Chemical Shift Probes

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