The effect of clobetasone butyrate and other topical steriods on skin thickness of the domestic pig

Winter, George D.; Wilson, Lyn

doi:10.1111/j.1365-2133.1976.tb05144.x

Cited by 18 publications

(8 citation statements)

References 5 publications

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“…Additionally, the IPS-induced abnormalities in epidermal proliferation and differentiation are consistent with known effects of increased GC. Indeed, in the epidermis (Winter and Wilson, 1976;Sheu et al, 1991), the PS-induced abnormalities are mimicked by short-term topical and systemic GC treatment, which also decreased epidermal cell proliferation and thickness (Kao et al, 2003). Finally, the IPS-induced decrease in lipid synthesis and LB production could be GC mediated, since GC similarly inhibits these parameters (Kao et al, 2003), and topical treatment with exogenous lipids normalized both permeability barrier homeostasis and SC integrity in GC-treated animals, indicating a similarly important pathophysiologic role for GC in reduced lipid production (Kao et al, 2003).…”

Section: Discussionmentioning

confidence: 98%

Mechanisms by Which Psychologic Stress Alters Cutaneous Permeability Barrier Homeostasis and Stratum Corneum Integrity

Choi

Brown

Crumrine

et al. 2005

Journal of Investigative Dermatology

181

144

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Although many skin disorders, including psoriasis and atopic dermatitis, are adversely affected by psychologic stress (PS), the pathophysiologic link between PS and disease expression remains unclear. Recent studies demonstrated PS-induced alterations in permeability barrier homeostasis, mediated by increased endogenous glucocorticoids. Here, we assessed the mechanisms by which PS alters stratum corneum (SC) function. Insomniac psychologic stress (IPS) altered both barrier homeostasis and SC integrity. IPS decreased epidermal cell proliferation, impaired epidermal differentiation, and decreased the density and size of corneodesmosomes (CD), which was linked to degradation of CD proteins (e.g., desmoglein1). Barrier compromise was linked to decreased production and secretion of lamellar bodies (LB), which in turn could be attributed to a decrease in de novo synthesis of epidermal lipids. Topical physiologic lipids (equimolar cholesterol, ceramides, and free fatty acids) normalized both barrier homeostasis and SC integrity in IPS mice, further evidence that lipid deficiency accounted for these functional abnormalities. Thus, PS inhibition of epidermal lipid synthesis results in decreased LB formation and secretion, as well as decreased CD, compromising both permeability barrier homeostasis and SC integrity. These studies suggest that topical treatment with epidermal physiologic lipids could be beneficial in stress-induced, barrier-associated dermatoses, such as psoriasis and atopic dermatitis.

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Section: Discussionmentioning

confidence: 98%

Mechanisms by Which Psychologic Stress Alters Cutaneous Permeability Barrier Homeostasis and Stratum Corneum Integrity

Choi

Brown

Crumrine

et al. 2005

Journal of Investigative Dermatology

181

144

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“…19 Atrophogenicity has been evaluated in mice, 32,33 in rats, 34,35 in pigs 36,37 and in dogs. 3 Castor and Baker showed as early as 1950 that topical application of hydrocortisone to rat skin caused dermal thinning.…”

Section: Determining the Atrophogenic Potential In Pre-clinical In VImentioning

confidence: 99%

Test systems for the determination of glucocorticoid receptor ligand induced skin atrophy

Schoepe

Schäcke

Asadullah

2011

Dermato-Endocrinology

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“…25,37,51 For previously mentioned reasons domestic pigs or minipigs are increasingly used for preclinical studies in investigative dermatology, which in turn has resulted in increased availability of new investigative tools such as monoclonal antibodies to cell surface markers or cytokines. 17,24,34,40,46,49,55,59,61 In addition, the possible use of genetically altered pigs as organ donors for humans created tremendous interest in swine immunology as an investigative tool.…”

mentioning

confidence: 99%

Morphologic and Immunohistochemical Features of Experimentally Induced Allergic Contact Dermatitis in Göttingen Minipigs

Vana

Meingassner

2000

Vet Pathol

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Abstract. Many preclinical studies in investigative dermatology are performed preferably in pigs because pig skin is more similar to human skin than is rodent skin. A frequently used model is allergic contact dermatitis (ACD); however, this T-cell-mediated skin condition so far is not well characterized in pigs. The present study is aimed at the evaluation of morphologic and immunohistochemical features of experimentally induced acute ACD in Göttingen minipigs using 2,4-dinitrofluorobenzene (DNFB) as a hapten. Eight minipigs were sensitized with 10% DNFB and challenged 2 weeks later at different sites with 1% DNFB. In addition to clinical examinations, cutaneous blood flow was quantified by laser Doppler velocimetry (Periflux PF3). These examinations were performed before challenge and 8, 24, 48, and 72 hours after challenge. Skin biopsies were taken at the same time points, fixed, sectioned, and stained with Giemsa for histologic evaluation, or with mouse anti-swine monoclonal antibodies (CD1, CD2, CD4, CD5, CD8, CD25, CD45, MHCII) and with one mouse anti-human monoclonal antibody (CD62E) cross-reacting with swine for immunohistochemical evaluation. Positively stained cells were counted per square millimeter of epidermis and dermis by using a video image analyzing system (Videoplan Kontron). Erythema and cutaneous blood flow peaked at 24 hours. The major epidermal changes most pronounced at 48 hours were acanthosis, spongiosis, intracellular edema, exocytosis, and abscesses mainly containing neutrophils and mononuclear cells (MNC). Perivascular infiltrates of MNC as well as neutrophils and eosinophils were the most significant dermal changes, with peak levels at 24-48 hours. In biopsies taken before challenge, CD1ϩ dendritic cells were found in similar numbers and locations as MHCII ϩ cells in the epidermis. In the epidermis the maximum CD1 ϩ cell decrease occurred at 24 hours whereas in the dermis the maximum increase in CD1 ϩ stained cells was seen at 72 hours. The dermal infiltrate (CD2 ϩ , CD5 ϩ , CD25 ϩ , and CD45 ϩ ) was most dense at 48 hours. Between 8 and 48 hours more CD4 ϩ were present than CD8 ϩ cells, whereas at 72 hours CD4 ϩ and CD8 ϩ cells were similar in numbers. These findings closely resemble changes in human ACD. Therefore, DNFB-induced ACD in Göttingen minipigs is considered to be an appropriate animal model to study immunopathologic mechanisms and pharmacologic intervention.Key words: Allergic contact dermatitis; 2,4-dinitrofluorobenzene; Göttingen minipig; histopathology; immunohistochemistry; skin inflammation; swine.Preclinical research in biomedical science uses laboratory animals to profile new compounds for possible uses as drugs. Therefore, laboratory animals serve either as models for the assessment of pharmacodynamic effects or, in advanced stages of drug development, for the identification of untoward effects (toxicities) of drug candidates. Therefore, the results obtained from animal pharmacology or animal toxicology studies should be reliably predictive for the situation in hum...

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The effect of clobetasone butyrate and other topical steriods on skin thickness of the domestic pig

Cited by 18 publications

References 5 publications

Mechanisms by Which Psychologic Stress Alters Cutaneous Permeability Barrier Homeostasis and Stratum Corneum Integrity

Mechanisms by Which Psychologic Stress Alters Cutaneous Permeability Barrier Homeostasis and Stratum Corneum Integrity

Test systems for the determination of glucocorticoid receptor ligand induced skin atrophy

Morphologic and Immunohistochemical Features of Experimentally Induced Allergic Contact Dermatitis in Göttingen Minipigs

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