The effect of co-culturing costal chondrocytes and dental pulp stem cells combined with exogenous FGF9 protein on chondrogenesis and ossification in engineered cartilage

Dai, Jiewen; Wang, Jia; Lu, Jingting; Zou, Duohong; Sun, Hao; Dong, Yuefu; Yu, Haisheng; Zhang, Lei; Yang, Tong; Zhang, Xiuli; Wang, Xudong; Shen, Guofang

doi:10.1016/j.biomaterials.2012.07.020

Cited by 66 publications

(78 citation statements)

References 49 publications

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“…DPSCs were obtained using direct cell outgrowth from dental pulp tissue explants and cultured as previously reported (16,17). Normal human third molars were obtained from adults (age, 16–25 years) in the Department of Oral and Maxillofacial Surgery, Stomatological Hospital, Shandong University (Jinan, China).…”

Section: Methodsmentioning

confidence: 99%

“…Erk1/2 and p38 have been demonstrated to be important in the proliferation and differentiation of DPSCs (14,15). A previous study revealed that fibroblast growth factor 9 (FGF9) was able to simultaneously promote the chondrogenesis of DPSCs by activating the phosphorylation of Erk1/2 (16). However, the exact effect of Erk1/2 and p38 on the chondrogenesis and osteognesis of DPSC remains to be completely elucidated.…”

Section: Introductionmentioning

confidence: 99%

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Differential effects of p38 and Erk1/2 on the chondrogenic and osteogenic differentiation of dental pulp stem cells

Duan²,

Fu³

et al. 2017

Molecular Medicine Reports

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The extracellular signal-regulated protein kinase 1/2 (Erk1/2) and p38 mitogen-activated protein-kinase pathways serve important roles in the regulation of osteogenic and chondrogenic differentiation in mesenchymal stem cells (MSCs). However, the exact mechanism remains unclear, and the effect is controversial. In the present study, the effects of Erk1/2 and p38 on the osteogenic and chondrogenic differentiation of dental pulp stem cells (DPSCs) were compared in vitro. The results indicated that inhibition of Erk1/2 is able to enhance the osteogenic differentiation of DPSCs and inhibit chondrogenic differentiation, whereas inhibition of p38 demonstrated the opposite effect. When compared with previous studies, the present study further confirmed that Erk1/2 and p38 serve important, but complicated, roles in regulating the differentiation of MSCs. Different chemical and physical stimuli, cell types, culture methods, times of inhibitor administration and the dosage of the inhibitor may influence the effect of Erk1/2 and p38 on the differentiation of MSCs. The present study aims to better understand the mechanisms that control the differentiation of MSCs and may be helpful in creating more effective tissue regeneration.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Differential effects of p38 and Erk1/2 on the chondrogenic and osteogenic differentiation of dental pulp stem cells

Duan²,

Fu³

et al. 2017

Molecular Medicine Reports

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“…A total of 10 4 HDPCs per well were plated in 48-well tissue culture plates (TCPs), and their viability on selected RBCs (RBC 0-60, RBC 50-20, RBC 70-0, Esthet-X and Z350 XT) with the size of Φ 10 mm × 1 mm was evaluated using MTT assay [19]. At different time points (1, 3, and 5 days) after initiation of culture, 50 μL MTT (0.5 mg/mL) was added into each well for additional 4 h incubation.…”

Section: Cell Viability Assaysmentioning

confidence: 99%

Investigation on the physical–mechanical properties of dental resin composites reinforced with novel bimodal silica nanostructures

Wang

Zhang

Liu

et al. 2015

Materials Science and Engineering: C

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“…In an attempt to identify the risk factors of oral cancer, researchers have found that smoking and alcohol consumption are the most notorious factrs. [1][2][3][4][5][6][7][8][9] However, oral cancer, like similar cancers, has multifactorial etiology. 1 Genetic factors and tobacco chewing also play an important role.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Fibroblast Growth Factor 9 on the Osteogenic Differentiation of Calvaria-Derived Mesenchymal Cells

Lu¹,

Dai²,

Wang³

et al. 2014

Journal of Craniofacial Surgery

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Fibroblast growth factor 9 (FGF9) plays complicated and crucial roles in bone formation, and the biologic effect of FGF9 may depend on the gene dosage, developmental stage, cell type, or interactions with other cytokines. In this study, we demonstrated that FGF9 enhanced the phosphorylation of extracellular regulated protein kinases 1/2 in calvaria-derived mesenchymal cells. However, the inhibitory effect of FGF9 on the osteogenic differentiation of calvaria-derived mesenchymal cells did not depend on the phosphorylation of extracellular regulated protein kinases 1/2. Combined with the previous findings that FGF9 promotes dental pulp stem cells chondrogenesis in vitro, we suggest that FGF9 may be applied to promote chondrogenesis and inhibit osteogenesis in mesenchymal stem cells in vitro.

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The effect of co-culturing costal chondrocytes and dental pulp stem cells combined with exogenous FGF9 protein on chondrogenesis and ossification in engineered cartilage

Cited by 66 publications

References 49 publications

Differential effects of p38 and Erk1/2 on the chondrogenic and osteogenic differentiation of dental pulp stem cells

Differential effects of p38 and Erk1/2 on the chondrogenic and osteogenic differentiation of dental pulp stem cells

Investigation on the physical–mechanical properties of dental resin composites reinforced with novel bimodal silica nanostructures

The Effect of Fibroblast Growth Factor 9 on the Osteogenic Differentiation of Calvaria-Derived Mesenchymal Cells

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