The Effect of Combined Diet Containing n-3 Polyunsaturated Fatty Acids and Silymarin on Metabolic Syndrome in Rats

Poruba, Martin; Anzenbacher, Pavel; Rácová, Zuzana; Oliyarnyk, Olena; Hüttl, Martina; Malínská, Hana; Marková, Irena; Gurská, Soňa; Kazdová, Ludmila; Večeřa, Rostislav

doi:10.33549/physiolres.934322

Cited by 11 publications

(9 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Moreover, the combination of silymarin and n-3 fatty acid supplementation may enhance the antihyperlipidemic effect in rats with metabolic syndrome [36]. These discrepancies with our results are probably due to the fact that different breeds and animals with induced liver disorders were used, rather than the healthy animals in our study (EXP1).…”

Section: Discussioncontrasting

confidence: 78%

Effects of Silybin Supplementation on Nutrient Digestibility, Hematological Parameters, Liver Performance, and Liver-specific mi-RNA Concentration in Dogs

Gogulski

Cieślak

Grabska

et al. 2021

Preprint

View full text Add to dashboard Cite

BackgroundHepatopathies are an important group of disorders in dogs, where proper nutritional care is crucial. Supplementation with a hepatoprotectant like silybin can improve liver performance, subsequently it should not interfere with nutrient digestibility. The purpose of the study was to investigate the effect of either pure silybin or commercial hepatoprotectant on nutrients digestibility, liver performance and health status in healthy dogs (EXP1). Moreover, the second experiment (EXP2) investigated the effect of commercial hepatoprotectant on liver laboratory test and liver-associated miRNA concentrations in dogs with idiopathic liver disorder. ResultsNutrient digestibility was not affected by treatment in EXP1. Supplementation did not affect serum fatty acid profile, with the exception of C20:5 n3. The levels of liver markers such as ALT, AST and GGT significantly decreased. In EXP2, supplementation with commercial hepatoprotectant containing silybin improved liver laboratory test. A decrease was observed in liver serum markers and miR122 concentration.ConclusionsEXP1 confirmed that silybin (whether pure or as a commercial hepatoprotectant) does not interfere with digestion and subsequently exerts no detrimental effect on dogs’ health and metabolism. In EXP2, dietary supplementation with commercial hepatoprotectant containing silybin resulted in decreased activity of serum liver markers, accompanied by a decrease in the concentration of liver-specific miRNA molecules. Liver performance was consequently improved. Silybin supplementation can thus serve as an effective therapeutical tool in dogs with hepatopathies.

show abstract

Section: Discussioncontrasting

confidence: 78%

Effects of Silybin Supplementation on Nutrient Digestibility, Hematological Parameters, Liver Performance, and Liver-specific mi-RNA Concentration in Dogs

Gogulski

Cieślak

Grabska

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…However, increased gene expression of the Abcg8 transporter indicated higher cholesterol secretion from hepatocytes into bile. ABCG5/8 transporters not only play an important role in sterol absorption and excretion but also provide an important pathway for cholesterol elimination [ 32 , 33 ]. While transcriptional factor SREBF1 is involved in fatty acid synthesis, SREBF2 regulates genes affecting cholesterol metabolism, the most important being HMGCR and the LDL receptor.…”

Section: Discussionmentioning

confidence: 99%

Ovariectomy-Induced Hepatic Lipid and Cytochrome P450 Dysmetabolism Precedes Serum Dyslipidemia

Malínská

Hüttl

Miklánková

et al. 2021

IJMS

View full text Add to dashboard Cite

Ovarian hormone deficiency leads to increased body weight, visceral adiposity, fatty liver and disorders associated with menopausal metabolic syndrome. To better understand the underlying mechanisms of these disorders in their early phases of development, we investigated the effect of ovariectomy on lipid and glucose metabolism. Compared to sham-operated controls, ovariectomized Wistar female rats markedly increased whole body and visceral adipose tissue weight (p ˂ 0.05) and exhibited insulin resistance in peripheral tissues. Severe hepatic triglyceride accumulation (p ˂ 0.001) after ovariectomy preceded changes in both serum lipids and glucose intolerance, reflecting alterations in some CYP proteins. Increased CYP2E1 (p ˂ 0.05) and decreased CYP4A (p ˂ 0.001) after ovariectomy reduced fatty acid oxidation and induced hepatic steatosis. Decreased triglyceride metabolism and secretion from the liver contributed to hepatic triglyceride accumulation in response to ovariectomy. In addition, interscapular brown adipose tissue of ovariectomized rats exhibited decreased fatty acid oxidation (p ˂ 0.01), lipogenesis (p ˂ 0.05) and lipolysis (p ˂ 0.05) despite an increase in tissue weight. The results provide evidence that impaired hepatic triglycerides and dysregulation of some CYP450 proteins may have been involved in the development of hepatic steatosis. The low metabolic activity of brown adipose tissue may have contributed to visceral adiposity as well as triglyceride accumulation during the postmenopausal period.

show abstract

“…Hypertriglyceridemia has been evaluated as an important factor in the formation of MetS, and was found to be significantly related to development of MetS, insulin resistance and a marker of chronic inflammation 21,22 . Many studies have shown that n‐3PUFAs in salmon phospholipids could effectively reduce TG levels in mice induced by HF diet to protect against hypertriglyceridemia 23‐25 . However, we found that the levels of TG and DG were significantly increased in the SA4 group versus HF group mice.…”

Section: Discussionmentioning

confidence: 63%

Intervention of 4% salmon phospholipid on metabolic syndrome in mice based on colonic lipidomics analysis

Cao

Chen

Wang³

et al. 2021

J Sci Food Agric

View full text Add to dashboard Cite

BACKGROUND The incidence of metabolic syndrome (MetS) is increasing, and n‐3 polyunsaturated fatty acids (PUFAs) in salmon (Oncorhynchus) phospholipids can effectively reduce the risk of MetS. RESULTS Under the intervention of 4% salmon phospholipid, the levels of fasting blood glucose (FBG), insulin, monocyte chemoattractant protein‐1 (MCP‐1), interleukin‐6 (IL‐6) and tumor necrosis factor‐α (TNF‐α) were significantly reduced in the plasma of MetS mice, whereas adiponectin was significantly increased. By screening, we found that the 18 differential metabolites, consisting of seven triglycerides (TGs), six diglycerides (DGs), one phosphatidylethanolamine (PE), three sphingomyelins (SMs) and one eicosanoid, could be the key differential metabolites, and two metabolic pathways were significantly affected: glycerolipid metabolism and glycerophospholipid metabolism. CONCLUSION 4% salmon phospholipids could affect MetS by inhibiting insulin resistance, reducing inflammatory factors and promoting the synthesis of PE, yet the mechanism required further study. Our results could help in the treatment of MetS. © 2021 Society of Chemical Industry.

show abstract

The Effect of Combined Diet Containing n-3 Polyunsaturated Fatty Acids and Silymarin on Metabolic Syndrome in Rats

Cited by 11 publications

References 37 publications

Effects of Silybin Supplementation on Nutrient Digestibility, Hematological Parameters, Liver Performance, and Liver-specific mi-RNA Concentration in Dogs

Effects of Silybin Supplementation on Nutrient Digestibility, Hematological Parameters, Liver Performance, and Liver-specific mi-RNA Concentration in Dogs

Ovariectomy-Induced Hepatic Lipid and Cytochrome P450 Dysmetabolism Precedes Serum Dyslipidemia

Intervention of 4% salmon phospholipid on metabolic syndrome in mice based on colonic lipidomics analysis

Contact Info

Product

Resources

About