The Effect of Combined Treatment with Carbonation, Heating, and Monoglycerol Fatty Acid Esters on the Inactivation and Growth Inhibition of Geobacillus stearothermophilus Spores

Nakai, Tomoe; Tani, Saburo; Klangpetch, Wannaporn; Noma, Satoshi; Igura, Noriyuki; Shimoda, Mitsuya

doi:10.3136/fstr.20.273

Cited by 5 publications

(5 citation statements)

References 17 publications

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“…We previously demonstrated that the addition of monoglycerol-caprate (MC 10 ) and monoglycerol-laurate (MC 12 ) increased the inactivation effect of carbonation with heating (CH) (80℃, 5 MPa, 30 min) on B. subtilis spores, and the combination treatment prevented growth during 30-day storage . We also revealed the significant inactivation effect of combined treatment of CH with MC 10 or MC 12 on Bacillus cereus, B. coagulans and Geobacillus stearothermophilus spores Nakai et al, 2014). However, the mechanism of the enhanced inactivation effect is still uncertain.…”

Section: Introductionmentioning

confidence: 84%

Enhanced Inactivation of Bacillus subtilis Spores by Carbonation with Heating in the Presence of Monoglycerol-caprate

Noma

Tani

Nakai

et al. 2015

FSTR

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Section: Introductionmentioning

confidence: 84%

Enhanced Inactivation of Bacillus subtilis Spores by Carbonation with Heating in the Presence of Monoglycerol-caprate

Noma

Tani

Nakai

et al. 2015

FSTR

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“…FAEs are widely used as food additives and are known to have a bacteriostatic effect (Shibasaki, 1979). In our previous studies, we observed a > 3 log-order inactivation effect on Bacillus and Geobacillus spores when carbonation under heating (CH) was performed in the presence of 2 mM monoglycerol caprate (MC10), an FAE (Klangpetch et al, 2013;Nakai et al, 2014). However, the bactericidal mechanism of the combination treatment of carbonation with MC10 against bacterial spores remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Inactivation of Bacillus subtilis Spores by Carbonation with Glycerin Fatty Acid Esters

Hirokado

Noma

Soh

et al. 2018

FSTR

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We investigated the effect of carbonation treatment with heating (CH) in the presence of glycerin fatty acid esters on Bacillus subtilis spores. The inactivation effect of CH was the highest in the presence of monoglycerol monocaprate (MC10). Monoglycerol monolaurate showed the highest bacteriostatic effect. The resistance of spores to CH with MC10 did not change with pre-heat activation. Freshly prepared spores from the surviving spores following CH with MC10 showed resistance similar to that of normal spores. The resistance of spores might be affected by the composition of the spore-forming medium. CH with MC10 enhanced adhesion of MC10 to spores and dipicolinic acid (DPA) release from spores, but did not induce DNA fragmentation. Inactivation of B. subtilis spores by CH with MC10 may be attributable to the impairment of physiological germination and/or decrease in heat resistance, which were induced by enhanced MC10 adhesion and the resultant DPA release.

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“…The addition of nisin enhances the inactivation effects of carbonation under heating on B. subtilis spores (Rao et al, 2016). The inactivation effect of carbonation under heating, on Bacillus and Geobacillus spores, is increased by the addition of fatty acid esters (Klangpetch et al, 2013;Nakai et al, 2014). These motivated us to investigate the inactivation effect of carbonation in the presence of various bacteriostatic agents.…”

Section: Introductionmentioning

confidence: 99%

Control of Bacillus subtilis Spores by Intermittent Treatment Using Heating after Carbonation in the Presence of Germinants and Bacteriostatic Agents

Tominaga

Noma

Igura

et al. 2018

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We investigated the inactivation effect of intermittent treatments using carbonation under heating (CH) and second heating (HT), in the presence of germinants or monoglycerol caprate (MC10) on Bacillus subtilis spores. Intermittent treatments by CH, followed by incubation and a second HT, in the presence of germinants showed up to 2.6 log-order inactivation effects on spores. Compared with the effects of glycine, sodium benzoate, and potassium sorbate, MC10 increased the inactivation effect of CH. MC10 decreased the D value and activation energy for inactivation of the spores. Intermittent treatments with MC10, conducted by a CH, followed by HT, inactivated spores by 3.6 log-orders. CH with MC10 induced the release of dipicolinic acid (DPA) and increased the stainability of the spores by DAPI. This suggests that germinationlike phenomena, during CH, were included in the inactivation effects. Spores, which survived the intermittent treatment combined with MC10, did not proliferate in a liquid medium.

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The Effect of Combined Treatment with Carbonation, Heating, and Monoglycerol Fatty Acid Esters on the Inactivation and Growth Inhibition of Geobacillus stearothermophilus Spores

Cited by 5 publications

References 17 publications

Enhanced Inactivation of <i>Bacillus subtilis</i> Spores by Carbonation with Heating in the Presence of Monoglycerol-caprate

Enhanced Inactivation of <i>Bacillus subtilis</i> Spores by Carbonation with Heating in the Presence of Monoglycerol-caprate

Inactivation of <i>Bacillus subtilis</i> Spores by Carbonation with Glycerin Fatty Acid Esters

Control of <i>Bacillus subtilis</i> Spores by Intermittent Treatment Using Heating after Carbonation in the Presence of Germinants and Bacteriostatic Agents

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