The production of corn-based ethanol in the U.S. has dramatically increasied in recent years, and consequently so has the quantity of coproduct feed ingredients generated from this segment of the grain processing industry. These streams are almost exclusively utilized as livestock feed, which partially offsets the need for corn in feed rations, but other value-added applications do exist. Because of its use as an animal feed, considerable research has been conducted into the nutritional properties, but to a lesser extent the physical and flowability properties of commercially-produced distillers dried grains with solubles (DDGS). There can be occasions when the quality of coproducts is not consistent. Thus questions regarding the influence of processing operations on the resulting coproduct characteristics must be examined. The objective of this research was to conduct extensive physical and flowability property analyses on DDGS samples which were produced under varying conditions in a pilot plant-scale ethanol plant, in order to investigate the effects of various manufacturing operations (specifically ethanol production and drying conditions) on the resulting properties of the DDGS. Using various laboratory methods, a variety of properties, including bulk density and angle of repose, were determined. DDGS fat content was highly correlated with aerated and packed bulk densities, which indicates that fat level plays a key role in flowability behavior. Future studies should examine this potential relationship in more depth, especially as the industry has moved to fat reduction via oil separation processes.