The Effect of Control-released Basic Fibroblast Growth Factor in Wound Healing

Abstract

Background:Basic fibroblast growth factors (bFGFs) play a crucial role in wound healing by promoting fibroblast proliferation and neovascularization. However, drawback of bFGF is short half-life in free form. Gelatin has a capability of sustaining growth factors, which are gradually released while degradation. The purpose of this study is to see whether bFGF-impregnated gelatin sheet is effective in a murine model and whether it could also be available for patients in a safe manner.Methods:Full-thickness skin defect was created on C57BL/6J mice and covered with bFGF with gelatin sheet (group A), bFGF without gelatin sheet (group B), phosphate buffer saline (PBS) with gelatin sheet (group C), and only PBS (group D). Wound healing was evaluated in terms of percent wound closure, granulation thickness, wound maturity, and vascular density. Clinical trial was conducted for patients who received either acute or chronic ulcers. The sheets were put onto the wounds and covered by hydrocolloid dressing, which was changed weekly.Results:Groups A and B exhibited better wound healing than groups C and D in all aspects. Moreover, group A showed better results than group B at day 7 in terms of wound closure, collagen maturity, and vascularity. Efficacy without any adverse events was found in the clinical series.Conclusions:These findings suggest that control-released bFGF using gelatin sheet is effective for promoting wound healing. Such therapeutic strategy was considered to offer several clinical advantages including rapid healing and reduction of the dressing change with less patient discomfort.

Single injection of basic fibroblast growth factor to treat severe vocal fold lesions and vocal fold paralysis

“…One promising growth factor is basic fibroblast growth factor (bFGF), which is a member of the fibroblast growth factor family . Its functions in developmental processes include mesoderm induction, anteroposterior patterning, limb development, neural induction, neural development, mature tissues/systems angiogenesis, keratinocyte organization, and wound‐healing processes . As a commercial‐based medicine, bFGF has been widely used to treat skin ulcers and bed sores.…”

“…14 Its functions in developmental processes include mesoderm induction, anteroposterior patterning, 15 limb development, neural induction, neural development, 16 mature tissues/ systems angiogenesis, keratinocyte organization, and wound-healing processes. 17 As a commercial-based medicine, bFGF has been widely used to treat skin ulcers and bed sores. Hirano et al 18 also confirmed that bFGF stimulates HA production and suppresses the production of collagen by the vocal fold fibroblasts.…”

Drug Delivery Systems for Wound Healing

“…Interestingly, the group has also investigated the sustained release of both reactive oxygen species (ROS) [54], and antioxidants [55] into the wound bed. FGF-2 impregnated pure gelatin sheets were also demonstrated to improve the granulation region thickness and collagen density in diabetic rat wounds [56]. These sheets also demonstrated efficacy in several patients with chronic non-healing wounds, however a randomized controlled clinical trial is necessary to conclusively assess the benefit of this therapy.…”

Therapeutic Angiogenesis by Growth Factors and Bone Marrow Mononuclear Cells Administration: Biological Foundation and Clinical Prospects

“…В ком-бустиологии -при лечении ожогов -использова-ние препаратов на основе FGF2 снижало частоту формирования выраженных келлоидных рубцов [100][101][102]. Применение FGF2 значительно ускоряет процессы заживления термического повреждения кожи, снижает число необходимых санаций ожого-вой раны, уменьшает дискомфортные ощущения со стороны пациента [103][104][105].…”