The effect of cranberry juice and a cranberry functional beverage on the growth and metabolic activity of selected oral bacteria

Abstract

Background The oral microbiota is a significant risk indicator for oral diseases, such as dental caries and periodontal inflammation. Much attention is presently paid to the development of functional foods (e.g. beverages containing cranberry constituents, or foods containing probiotics) that may serve as adjuncts for oral disease treatments (e.g. periodontitis and caries). Cranberry fruit, due to its unique chemical composition and antimicrobial potential, is a possible ingredient of such foods. The study aimed to investigate the effects of cranberry juice (CJ) and a cranberry functional beverage (mixture of 80% v/v apple juice, 20% v/v cranberry juice, and 0.25 g/100 mL ground cinnamon; CFB) on the growth and metabolic activity of selected oral bacteria. Methods Serial dilution pour plate method (SDPP) was used to examine the effect of CJ and CFB on the growth of Actinomyces naeslundii, Streptococcus mutans, and Lactobacillus paracasei subsp. paracasei. 48-h electrical impedance measurements (EIM) during the cultivation of A. naeslundii were applied to evaluate the utility of the method as a rapid alternative for the assessment of the antimicrobial potential of cranberry beverages. Results The tested bacteria differed in their susceptibility to the antimicrobial action of CJ and CFB, with L. paracasei subsp. paracasei being least vulnerable to CFB (according to SDPP). Although CJ at a concentration of 0.5 mL/mL, showed a bactericidal effect on the growth of S. mutans, A. naeslundii was more sensitive to CJ (SDPP). Its inhibitory effect on A. naeslundii was seen even at concentrations as small as 0.03125–0.125 mL/mL (SDPP and EIM). On the other hand, S. mutans seemed to be more vulnerable to CFB than A. naeslundii (SDPP). Conclusions CFB may be considered an adjunct in the treatment of oral diseases due to its action against selected oral pathogens, and not against the presumably beneficial L. paracasei subsp. paracasei. Bioelectrical impedance measurements appear to be a quick alternative to evaluating the antimicrobial activity of fruit beverages, but their utility should be confirmed with tests on other bacteria.

From medical strategy to foodborne prophylactic strategy: Stabilizing dental collagen with aloin

“…As illustrated in Figure 2a, peak I intensities were higher in the XRD pattern of the A-DDC group, suggesting that aloin may induce a shorter lateral spacing between collagen molecules (Green et al, 2017). This conjecture is further supported by the observed increase in 2θ angles of the diffraction peak I and III in the A-DDC group, indicating that the addition of aloin reduces the spacing between collagen molecules (Ding et al, 2019;Nwambaekwe et al, 2021). Peak II, which can be seen at roughly 2θ ~20°, is due to the diffusive scattering caused by the presence of random-coil structures embedded within the collagen fibers (Giraud-Guille et al, 2000).…”

“…In the presence of bacteria and enzymes, as well as changes in temperature, apatite mineral crystals in the enamel and dentin gradually deteriorate, exposing the underlying organic dental collagen network (Wang, Wang, et al., 2022 ). When dental collagen is exposed, it becomes more vulnerable to external infections, and its triple‐helix structure deteriorates rapidly (Nowaczyk et al., 2021 ). Loosening of the collagen structure and the high porosity of the collagen network facilitate the diffusion of infectious substances.…”

Effect of flavonoids from grape seed and cranberry extracts on the microbiological activity of Streptococcus mutans: a systematic review of in vitro studies

“…Bias evaluation was performed according to the parameters established in Table 2 . The main risk of bias was found in that study limitations were either not reported in the study report [ 24 – 26 , 29 , 36 , 37 , 39 , 41 – 43 ] or were described briefly [ 27 , 28 , 31 – 34 , 40 , 44 ]. Secondly, there was a failure to include relevant ethical aspects such as institutional ethics committee approval, or patient informed consent for saliva sample collection was not clearly detailed in the article text, or a declaration of project financing and conflict of interest was absent [ 27 – 29 , 31 – 37 , 39 – 42 , 44 ].…”

Vaccinium Species (Ericaceae): Phytochemistry and Biological Properties of Medicinal Plants

“…NDM can inhibit the proliferation of P.gingivalis , T.forsythia , and T.denticola in periodontal pockets. Unusual production of cytokines by host cells caused by periodontopathogens damage tooth-supporting tissues; studies have demonstrated that cranberry was an excellent inhibitor of pro-inflammatory cytokine and chemokine replies caused by lipopolysaccharides [ 124 , 133 , 134 ].…”