The effect of cryopreservation on goat semen characteristics related to sperm freezability

Dorado, J.; Muñoz-Serrano, A.; Hidalgo, M.

doi:10.1016/j.anireprosci.2010.04.182

Cited by 46 publications

(37 citation statements)

References 37 publications

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“…These findings are similar to those obtained by Karagiannidis et al (2000) who worked with Alpine, Saanen and Damascus goats, and that reported by Roca et al (1992), Pérez & Mateos (1996), Zarazaga et al (2009) andDorado et al (2010) for other Spanish goat breeds. However, the seasonal changes in plasma testosterone were not associated with any variation in TW, the percentage of ejaculating bucks or active bucks, ejaculation latency, or ejaculate volume.…”

supporting

confidence: 91%

Seasonal changes in reproductive activity, sperm variables and sperm freezability in Blanca Andaluza bucks

Calvo

Jorquera

Santiago-Moreno

et al. 2015

Span. j. agric. res.

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Interest in the preservation of endangered breeds such as the Blanca Andaluza goat, has increased and some steps should be therefore taken to ensure it. The study was designed to determine the seasonal reproductive pattern of Blanca Andaluza bucks, and whether this affects the quality of their semen and its freezability over the year. Seven bucks were used and their body weight, testicular weight, plasma testosterone concentration and fresh sperm quality determined every week. The collected sperm was cryopreserved and stored; it was then thawed and the same sperm quality variables measured every fortnight. High plasma testosterone concentrations were recorded during the summer and autumn, and low concentrations were recorded during winter and spring (p<0.001). No differences were seen between seasons in terms of the percentage of bucks ejaculating, the percentage of active bucks, or ejaculate volume. However, the sperm concentration, the total number of sperm per ejaculate, and the values for most fresh sperm variables were lower during the winter period (at least p<0.05). After freezing-thawing, the quality of winter-collected sperm was better, in some respects, than that of summer-collected sperm (at least p<0.05). These results reveal that Blanca Andaluza bucks show seasonal reproductive activity in terms of their plasma testosterone concentration, but no clear change in their sexual behaviour between seasons was observed. The values of fresh sperm variables also vary over the year, reaching their lowest during winter. However, after freezing-thawing, winter-collected sperm is of overall better quality than sperm collected during the summer.Additional key words: endangered goat breed; testosterone; seasonality; fresh semen; cooled semen; semen cryopreservation.

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supporting

confidence: 91%

Seasonal changes in reproductive activity, sperm variables and sperm freezability in Blanca Andaluza bucks

Calvo

Jorquera

Santiago-Moreno

et al. 2015

Span. j. agric. res.

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“…Similarly, ALH was shown to affect the outcome of IVF in humans (Barlow et al, 1991) and rats (Moore and Akhondi, 1996). On contrary, few researchers failed to determine an association between motion parameters of semen samples and fertility after AI in bull (Bailey et al, 1994), sheep (Sanchez-Partida et al, 1999), goat (Dorado et al, 2010) and stallion (Nie et al, 2002), due in part to the several factors that influence the fertility of animals (Gadea, 2005). Nevertheless, the present study has shown that the sperm kinematics, especially the sperm velocity parameters, differ between high and low fertile buffalo bulls and stress the importance of using the CASA techniques to detect differences in sperm kinematics between high and low fertile buffalo bulls, which would help in identifying ejaculates with high fertilizing potential.…”

Section: Resultsmentioning

confidence: 99%

Computer assisted sperm analysis: Relationship between the movement characteristics of buffalo spermatozoa and sire fertility

Singh

Kumaresan

et al. 2016

IJAR

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The present study was undertaken to identify the differences in sperm kinematics between buffalo bulls with different fertility ratings. Murrah buffalo bulls (n=9) that were routinely used for breeding purpose under progeny testing programme were utilized for the study. Bull fertility was determined based on in vivo fertility trials and the conception rates (CR) were adjusted for different non-genetic parameters. Based on the adjusted CR, bulls were classified into high, medium and low fertile group. Frozen semen samples of these bulls were obtained and sperm kinematic parameters were assessed using a computer assisted sperm analyzer. The kinematic parameters analyzed included the curvilinear velocity (VCL), the linear velocity (VSL), the average path velocity (VAP), the amplitude of lateral head displacement (ALH), the linearity (LIN), the straightness coefficient (STR) and the beat cross frequency (BCF). In high fertile bulls, the proportion of motile spermatozoa was higher (p<0.001) than the medium and low fertile bulls. The VAP and VCL of sperm motion were significantly higher (P<0.05) in high fertile bulls compared to either medium or low fertile bulls. The VSL was significantly lower in low fertile bulls (P<0.005) compared to either high or medium fertile bulls. Spermatozoa from high fertile bulls had significantly higher (P<0.05) BCF, STR, ALH and LIN compared to either medium or low fertile bulls. Buffalo bull fertility was significantly and positively correlated with sperm motility, VAP, VSL, VCL and ALH.

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“…CASA provides an objective and reproducible data on a number of sperm motion parameters and enhance the value of motility assessment to fertility prognosis resulting in high correlations among several CASA motility parameters [6,7]. Since the identification of ejaculates as suitable, or not suitable is based on fresh and post-thaw semen quality [8], the study was conducted to evaluate the motility and kinematic parameters in the neat, diluted and frozen thaw semen of Sirohi buck.…”

Section: Introductionmentioning

confidence: 99%

Assessment of motion and kinematic characteristics of frozen-thawed Sirohi goat semen using computer-assisted semen analysis

Anand¹,

Yadav²

2016

Vet World

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Aim:The aim was to determine the motion and kinematics characteristic of frozen-thawed spermatozoa in Sirohi goat using computer-assisted semen analysis. Materials and Methods:A study was carried out in Sirohi buck. Semen collection was made biweekly from each buck with the help of artificial vagina. A total of 12 ejaculates were collected from two bucks (six ejaculates from each buck). Freshly collected semen was pooled and later evaluated. The pooled semen sample was extended with standard glycerolated egg yolk tris extender and later subjected to a process of cryopreservation. The motion and kinematic characteristics of spermatozoa were studied during freez-thawing process.Results: Significantly (p<0.01) higher value of live percent, hypo-osmotic swelling test, and acrosomal integrity were recorded in neat semen followed by diluted and frozen thaw semen. The proportion of spermatozoa showing slow progression were the highest in the neat and diluted semen followed by rapid and non-progressively motile, while a reverse pattern was observed in the frozen thaw semen where the proportion of non-progressively motile spermatozoa were significantly (p<0.01) higher followed by slow and rapid progression. Conclusion:This study showed that the best results for motion, vitality, plasma membrane integrity, and acrosome status were obtained in the neat semen followed by diluted and frozen thaw semen. Further, the process of cryopreservation results in a shift of motility from slow to non-progressive in the post-thaw semen with a significant decrease in the path velocities when compared to neat and diluted semen. Hence, it can be concluded that freezing-thawing process reduces the motility and kinematic characters spermatozoa and may be an important factor affecting the fertilizing ability of spermatozoa resulting in poor conception rate after insemination in goats.

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The effect of cryopreservation on goat semen characteristics related to sperm freezability

Cited by 46 publications

References 37 publications

Seasonal changes in reproductive activity, sperm variables and sperm freezability in Blanca Andaluza bucks

Seasonal changes in reproductive activity, sperm variables and sperm freezability in Blanca Andaluza bucks

Computer assisted sperm analysis: Relationship between the movement characteristics of buffalo spermatozoa and sire fertility

Assessment of motion and kinematic characteristics of frozen-thawed Sirohi goat semen using computer-assisted semen analysis

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