The Effect Of Culture System On Embryonic Development and Aneuploidy Rate For Icsi Cases

Abstract

Background: Patients undergoing intracytoplasmic sperm injection (ICSI) or in vitro fertilization (IVF)cycles often suffer from recurrent implantation failure (RIF). To ensure live birth and embryonic viability, culture systems should be optimized in all ICSI and IVF cycles. The success of IVF can be affected by the culture systems employed for embryonic development. It has been argued that covering micro drops with oil in culture systems can prevent microbial infection, ensure suitable osmotic and pH conditions, and prevent the evaporation of the medium.Objectives: Comparing the embryonic development, euploidy rate ,and clinical outcome of using GPS Dishes covered with paraffine oil and SPL Dishes with mineral oil for patients with previous RIF.Patients and methods: the study was a prospective randomized controlled trial and the sample size consisted of 174 patients undergoing ICSI cycles at a private fertility center during the period between April 2017 and March 2020. Patients' embryos were randomly assigned into one of the two of the aforementioned groups. Results:The two groups weren't statistically significantly different in male age, female age, collected oocyte number, number of mature oocytes, and number of blastocysts undergoing preimplantation genetic diagnosis (PGD-A). For the preimplantation parameters of the embryos, fertilization, cleavage, and high-quality blastocyst rates were statistically significantly higher in the GPS Dishes with paraffin oil group than in the SPL Dishes covered with mineral oil group (77.5, 80.1, and 79.45%) (p = 0.012, and 0.001). Conclusion:we conclude that embryonic development can be enhanced by using GPS Dishes with paraffin oil overlying.

Development of Bovine Embryo Culture System for Maintaining Genetic Identity of Embryos

“…Such dishes helps with cell recovery and identification and can prevent micro drop issues such as aggregation or moving of drops. This GPS dish allows for the sharing of a media reservoir and also makes it easier to observe embryos because one embryo may be cultured in each partition [24,25]. It can take some time for the embryos in the bottom of the concave microwell to settle centrally because of gravity and that may facilitate the development of fewer number of COCs to embryo in GPS culture dish.…”