The EFFECT OF CULTURE TECHNIQUES OF HYPOXIC STEM CELL SECRETOME ON THE NUMBER OF GROWTH FACTOR TGF-ß, BMP-2, VEGF

Abstract

Background: Mesenchymal stem / stromal cell therapy (MSCs) is now an effective therapeutic modality for treating various diseases. In its application, stem cells require signaling molecules which can be growth factors, cytokines, or chemokines. Signal molecules work orderly and are greatly influenced by the physiological environment. Stem cell culture techniques with hypoxic conditions can produce growth factors close to physiological conditions in fractures. This study aims to perceive the different expressions of some growth factors in cultured normoxic and hypoxic BMSC.Methods: This study is an in vitro laboratory experimental study of normoxic Bone Marrow Stem Cells (BMSCs) and Hypoxic Bone Marrow Stem Cells (BMSCs) cultures. The BMSCs experimental unit was taken from rabbits and then propagated in vitro and cultured under two conditions, normoxia and hypoxia. Then the number of VEGF, TGF-β, BMP-2 growth fractures was observed using ELISA.Results: VEGF, TGF-β, and BMP-2 expressions showed significant differences between the normoxia and hypoxia groups. VEGF, TGF-β, and BMP-2 expression were higher in the hypoxia group compared with the normoxia group (p < 0.05).Conclusion: The expression analysis of TGFβ-1, VEGF, and BMP-2 growth factors in cultured BMSC were statistically significant between normoxic and hypoxic conditions. TGFβ-1, VEGF, and BMP-2 expressions increase in hypoxic conditions.