The effect of current used bone substitution materials and platelet-rich plasma on periosteal cells by ectopic site implantation: An in-vivo pilot study

Metzler, Philipp; Wilmowsky, Cornelius von; Zimmermann, Robert; Wiltfang, Jens; Schlegel, Karl Andreas

doi:10.1016/j.jcms.2011.07.012

Cited by 27 publications

(15 citation statements)

References 52 publications

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“… 9 There are conflicting studies within the literature on osteoinductive potential of β-TCP, 32 – 34 but it is noteworthy that its degradation products can cause inflammation and impair subsequent bone formation. 35 Prior studies have indicated that BG is not highly osteoinductive de novo, 36 but our study tested it in the context of a potent rhBMP-2 bone formation scenario. Aside from the lack of increased bone seen with TCP and BG alone, neither bioceramic showed significant synergy in combination with a bisphosphonate.…”

Section: Discussionmentioning

confidence: 99%

Bisphosphonate-adsorbed ceramic nanoparticles increase bone formation in an injectable carrier for bone tissue engineering

et al. 2015

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Sucrose acetate isobutyrate (SAIB) is a sugar-based carrier. We have previously applied SAIB as a minimally invasive system for the co-delivery of recombinant human bone morphogenetic protein-2 (rhBMP-2) and found synergy when co-delivering zoledronic acid (ZA) and hydroxyapatite (HA) nanoparticles. Alternative bioceramics were investigated in a murine SAIB/rhBMP-2 injection model. Neither beta-tricalcium phosphate (TCP) nor Bioglass (BG) 45S5 had a significant effect on bone volume (BV) alone or in combination with the ZA. 14C-labelled ZA binding assays showed particle size and ceramic composition affected binding with nano-HA > micro-HA > TCP > BG. Micro-HA and nano-HA increased BV in a rat model of rhBMP-2/SAIB injection (+278% and +337%), and BV was further increased with ZA–adsorbed micro-HA and nano-HA (+530% and +889%). These data support the use of ZA–adsorbed nanoparticle-sized HA as an optimal additive for the SAIB/rhBMP-2 injectable system for bone tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Bisphosphonate-adsorbed ceramic nanoparticles increase bone formation in an injectable carrier for bone tissue engineering

et al. 2015

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“…This factor is especially important when the biomaterials are applied to increase the bone volume for example in the case of the maxillary sinus floor elevation or alveolar crest augmentation, when the objective is to place dental implants [1, 2]. In order to investigate these important features several in vivo models [1, 3–5] have been developed.…”

Section: Introductionmentioning

confidence: 99%

Novel use of cranial epidural space in rabbits as an animal model to investigate bone volume augmentation potential of different bone graft substitutes

et al. 2016

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BackgroundThe success of bone augmentation to a major degree depends on the biomechanics and biological conditions of the surrounding tissues. Therefore, an animal model is needed providing anatomical sites with similar mechanical pressures for comparing its influence on different biomaterials for bone regeneration. The present report describes the new bone formation associated to biomaterial in a bursa created in the epidural space, between dura mater and cranial calvaria, under the constant pressure of cerebrospinal fluid.MethodsFive adult California rabbits were used for the trial. In each animal, two bursae were created in the epidural spaces, in the anterior part of the skull, below both sides of the interfrontal suture. The spaces between dura mater and cranial calvaria were filled with in-situ hardening biphasic calcium phosphate containing hydroxyapatite and beta tricalcium-phosphate (BCP), in-situ hardening phase-pure beta-tricalcium phosphate (β-TCP) or without any biomaterials (sham). After 90 days, the animals were sacrificed, and the defect sites were extracted and processed for histomorphometric analysis by optical and backscattered electron microscopy.ResultsThe cranial epidural spaces created (n = 10) could be preserved by the application both BCP (n = 3) and β-TCP biomaterials (n = 3) in all experimental sites. The sites augmented with BCP showed less new bone formation but a trend to better volume preservation than the sites augmented with β-TCP. However, the bone in the BCP sites seemed to be more mature as indicated by the higher percentage of lamellar bone in the sites. In contrast, the created space could not be preserved, and new bone formation was scarce in the sham-operated sites (n = 4).ConclusionThe experimental bursae created bilaterally in the epidural space allows comparing objectively bone formation in relation to biomaterials for bone regeneration under permanent physiological forces from cerebrospinal fluid pressure.

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“…This is in addition to the wide variability of existing preparation protocols and the lack of standardization measures [103,104]. The general consensus for clinical use of PRP is that it contain 5 times more than the concentration of platelets than in normal blood.…”

Section: Prp As An Endogenous Regenerativementioning

confidence: 99%

“…PRP could enhance de novo bone formation when combined with periosteum and Bio-Oss (deproteinized bovine bone) as the scaffold [104]. PRP could enhance de novo bone formation when combined with periosteum and Bio-Oss (deproteinized bovine bone) as the scaffold [104].…”

Section: Prp As An Endogenous Regenerativementioning

confidence: 99%

Bone Regeneration and Bioengineering

Backly

Mastrogiacomo²,

Cancedda³

2014

Regenerative Medicine Applications in Organ Transplantation

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The effect of current used bone substitution materials and platelet-rich plasma on periosteal cells by ectopic site implantation: An in-vivo pilot study

Cited by 27 publications

References 52 publications

Bisphosphonate-adsorbed ceramic nanoparticles increase bone formation in an injectable carrier for bone tissue engineering

Bisphosphonate-adsorbed ceramic nanoparticles increase bone formation in an injectable carrier for bone tissue engineering

Novel use of cranial epidural space in rabbits as an animal model to investigate bone volume augmentation potential of different bone graft substitutes

Bone Regeneration and Bioengineering

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