The effect of cycloheximide and 6-methylpurine on in vivo compatible and incompatible pollen tube growth in Lilium longiflorum

Ascher, Peter D.; Drewlow, L. W.

doi:10.1007/bf00282699

Cited by 11 publications

(2 citation statements)

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“…When carried in stigmatic exudate and injected at or after pollination, these substances tended to significantly retard compatible pollen tube growth at concentrations which did not affect incompatible tube lengths. tube growth (16). In the 26 experiments conducted with these materials (Table 4), compatible pollen tube growth was more sensitive than incompatible to RNA synthesis inhibition, suggesting that compatible pollen tube development requires RNA synthesis unnecessary for normal incompatible pollen January 1981 109 bExperiments with treatments reducing pollen tube growth to near zero cannot be subjected to standard analysis of variance since the variance is correlated to treatment.…”

Section: Resultsmentioning

confidence: 99%

“…Using various inhibitors of RNA or protein synthesis and either stigmatic exudate or distilled water as carriers, we found that both compatible and incompatible pollen tubes require protein synthesis for normal growth, that only compatible tubes require RNA synthesis, and that the style requires RNA synthesis to produce the selfincompatibility reaction in L. longiflorumt (14,16). The purpose of this presentation is to report the effects on pollen tube growth of a number of exogenous materials injected into L. longiflorum styles.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Exogenous Materials on Pollen Tube Growth in Lilium longiflorum Pistils

Ascher¹

1981

Environmental Health Perspectives

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JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range of content in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new forms of scholarship. For more information about JSTOR, please contact support@jstor.org.. The National Institute of Environmental Health Sciences (NIEHS) and Brogan & Partners are collaborating with JSTOR to digitize, preserve and extend access to Environmental Health Perspectives.With the use of stigmatic exudate or distilled water as carriers, various antimetabolites, inhibitors, and miscellaneous materials were injected into the hollow styles of detached Liliuna longiflorum pistils before, at, or after compatible or incompatible pollination. Pollen tube lengths were measured 48 hr after pollination with pollinated styles incubated at 22-230C. Substances considered inhibitors of protein synthesis in microbial systems significantly retarded both compatible and incompatible pollen tube growth while inhibitors of RNA synthesis tended to significantly inhibit compatible pollen tube growth with less or no effect on incompatible pollen tubes. Application of the inhibitors in stigmatic exudate at or after compatible pollination produced significant results at the lowest concentrations. Significant retardation of pollen tube growth also occurred after injection of 2,4-dinitrophenol, mercaptoethanol, indoleacetic acid, naphthaleneacetic acid, benzyladenine, dimethyl sulfoxide, or potassium or sodium iodide. Pollen tube growth in detached pistils of L. longiflorum may be useful as a bioassay in situ for screening biologically active materials. maximizes expression of virus symptoms. With care and a glasshouse or other suitable high-light facility, L. longiflorum can be flowered a second time in the same container. To do this, bulbs should be potted originally in standard containers at least 15 cm in diameter in a well drained medium such as equal parts soil, sphagnum peat moss, and perlite, and given a complete fertilizer regularly. After flowering the plants should be provided full light and the fertilization continued for 2-3 months. At this time plant tops should be cut at the soil level and the potted bulbs returned to cold storage to repeat the cycle.Large chromosomes, good correlation between anther length and meiotic stage, and synchrony of meiotic stage among anthers in a single bud make L. longiflorum an excellent subject for cytogentic studies (1-6). The Easter lily is also uniquely suited for studies of pollen-pistil interactions. Lily styles are hollow, lined with specialized secretary cells (7) on which pollen tubes grow from the stigma to the ovary. This hollow style facilitates pollen tube staining in that aqueous aniline blue (or other stain) can be injected into the style through the stigma with a needle and syringe (8). Refrigeration of aniline blue-stained pistils for 1 hr or more in-January 1981 1 07 This content downloaded from 62.122.79.22 on Tue

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of Exogenous Materials on Pollen Tube Growth in Lilium longiflorum Pistils

Ascher¹

1981

Environmental Health Perspectives

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Germination of Brassica pollen and expression of incompatibility in vitro

Ferrari

Wallace

1975

Euphytica

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Pollen derived from cabbage, broccoli, and collards (Brassica oleracea, var. capitata, Italica and acephala, respectively) will germinate in a synthetic medium consisting of sucrose, H3BO4, CaC12 and purified polyethylene glycol 20,000. The optimum levels of constituents varied from genotype to genotype. With one genotype of cabbage, a germination percentage of 84~,, and tube lengths of at least 3.5 mm long were obtained. Whereas pollen from broccoli germinated in 1 to 2 hours, cabbage pollen required a 3 to 4 hour incubation period. Optimum germination occurred at pH 5.8 and at a pollen density of 0.5 2.0 mg/ml for cabbage. The effect of flower age on pollen germination varied from genotype to genotype. Of 7 cabbage genotypes tested, pollen germination either increased, decreased or remained the same as flower age increased. Pollen germination in vitro required polyethylene glycol purified by dialysis or passage through Sephadex G-25. Cabbage stigmas release a heat-labile substance capable of inhibiting germination of selfpollen, but stigma extracts from a cross plant have no effect.

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Incorporation of radioactive label into nucleic acids of compatible and incompatible pollen tubes of Lilium longiflorum Thunb.

Campbell

Ascher

1975

Theoret. Appl. Genetics

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The effect of cycloheximide and 6-methylpurine on in vivo compatible and incompatible pollen tube growth in Lilium longiflorum

Cited by 11 publications

References 11 publications

Effects of Exogenous Materials on Pollen Tube Growth in Lilium longiflorum Pistils

Effects of Exogenous Materials on Pollen Tube Growth in Lilium longiflorum Pistils

Germination of Brassica pollen and expression of incompatibility in vitro

Incorporation of radioactive label into nucleic acids of compatible and incompatible pollen tubes of Lilium longiflorum Thunb.

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