The effect of DDT on the incorporation of glucose and glycine into various intermediates in DDT-resistant strains of Musca domestica L.

Protein synthesis in polymers from houseflies and the effect of 2,2-bis(p-chlorophenyl)-1,1,1-trichloroethane

“…DDT has been shown to increase the incorporation of 14C-labeled amino acids into total proteins of Musca domestica (Agosin et al, 1966). It was then considered of interest to establish the pattern of in vivo incorporation of 14C-labeled amino acids into polysomes in insects pretreated with DDT.…”

“…Groups of 40 insects were injected intrathoracically with 0.6 gl of 0.001 m neutral aqueous solutions of either L-phenylalanine-2-14C or leucine-1-14C (3 X 104 cpm/fly) as described previously (Agosin et al, 1966). After standing for periods indicated in tables and figures at 27°, polysomes were prepared and fractionated by sucrose density gradients as described above.…”

“…DDT Treatment. Acetone solutions of DDT were topically applied at the dose of 1 Mg/fly as described previously (Agosin et al, 1966). Analytical Procedures.…”

“…An important part of this paper deals with the in vivo and the in vitro effect of DDT on these polysome structures. Most of the work was conducted with two housefly strains (Fc and P2/sel) which have higher and DDT-inducible microsomal mixed-function oxidase activity (Agosin et al, 1966).…”

Biochemical aspects of comparative nutrition

“…DDT is detoxified by a NADPH-dependent microsomal oxidase (Agosin & Fine, 1965). Houseflies which show D D T resistance also have a greatly enhanced pentose phosphate pathway to ensure a plentiful supply of reduced NADPf (Agosin, Fine, Scaramelli, Ilivicky & Aravena, 1966). An increased turnover of glutathione also results from the increased pentose phosphate pathway and an enhanced protein synthesis, perhaps to synthesize detoxifying enzymes (Agosin & Fine, 1965).…”