The effect of denervation, pituitrin and varied cation concentration gradients on the transport of cations and water in kidney slices

Cort, J. H.; Kleinzeller, A.

doi:10.1113/jphysiol.1956.sp005586

Cited by 20 publications

(17 citation statements)

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“…= concentration of Na in the medium in m-moles/l., and Wtot. =total tissue water in ml./100 g DS (calculated from wet and dry wt., Cort & Kleinzeller, 1956). Average percentage values of extracellular tissue Na calculated on this basis are shown in Fig.…”

Section: Methodsmentioning

confidence: 99%

“…Standard techniques were used for the measurement of Qo2, as previously described (Cort & Kleinzeller, 1956) RESULTS Fig. 1 shows the rate of appearance of 24Na in the medium during a control 'incubation' carried out at 0°C (inhibition of active transport) under otherwise identical leaching conditions as with slices incubated at higher temperatures.…”

Section: Methodsmentioning

confidence: 99%

“…Correction was also made for source geometry (see Cort & Kleinzeller, 1956) so that absolute values could be obtained (an end-window G.-M. tube was used) with further correction being made for air and mica absorption of the beta and gamma energy levels arising from disintegration of 24Na and 42K (data from standard tables). These activities were divided by the dry weight (mg) of tissue in the respective samples x 105 (for conversion to activities per 100 g dry solids).…”

Section: Methodsmentioning

confidence: 99%

“…Tissue electrolytes were estimated by flame photometry, as described by Cort & Kleinzeller (1956). Correction for the intracellular partition of total tissue Na was calculated on the basis of the data of Conway, Geoghegan & McCormack (1955) and Whittam (1956) that 25 % oftissue water is extracellular before both and after leaching at 00 C, as follows: Na1c0 = Nat0…”

Section: Methodsmentioning

confidence: 99%

“…Whittam & Davies (1954) have shown that the rates of uptake of 24Na and 42K were very much greater at 37 than at 0°C, as would be expected if the transport process was associated with a biochemical reaction rather than a physical process such as simple diffusion through a permeable membrane, on which the effect of temperature would be smaller.It has been previously suggested (Cort & Kleinzeller, 1956 Kleinzeller & Cort, 1957 a, b) that only Na is actively transported out of renal tubular cells (at the basal membrane), while K appears to behave more passively according to gradients of concentration and electrical potential across the cell membrane. An attempt has been made to examine this question further by observation of the effect of three different temperatures (15, 25 and 300 C) on the two fluxes of greatest physiological interest, the efflux of Na (Mo, Na) and the accumulation of K (Mi, K) during incubation of kidney cortex slices.…”

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confidence: 99%

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The effect of temperature on the transport of sodium and potassium by kidney cortex slices

Cort¹,

Kleinzeller²

1958

The Journal of Physiology

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The maintenance of differences in electrolyte composition between intracellular and extracellular fluids in kidney slices (predominantly tubular cells) is a process which requires the expenditure of metabolic energy (see review by Davies, 1954). Whittam & Davies (1954) have shown that the rates of uptake of 24Na and 42K were very much greater at 37 than at 0°C, as would be expected if the transport process was associated with a biochemical reaction rather than a physical process such as simple diffusion through a permeable membrane, on which the effect of temperature would be smaller.It has been previously suggested (Cort & Kleinzeller, 1956 Kleinzeller & Cort, 1957 a, b) that only Na is actively transported out of renal tubular cells (at the basal membrane), while K appears to behave more passively according to gradients of concentration and electrical potential across the cell membrane. An attempt has been made to examine this question further by observation of the effect of three different temperatures (15, 25 and 300 C) on the two fluxes of greatest physiological interest, the efflux of Na (Mo, Na) and the accumulation of K (Mi, K) during incubation of kidney cortex slices. (In this paper the symbol M represents amount transported, whereas M' represents the amount transported in unit time.) Change of temperature may be expected to influence the rate of transport at the cell membrane in a complex manner since permeability, active transport and potential gradient (Q) will probably all be affected. 'D cannot be independently varied in tissue slices, and has not been measured here. In order to minimize the effect of T on D a kinetic, rather than a steadystate, approach has been adopted, the rate of transport being extrapolated to zero time by means of tangents to the curves of quantity transported versus time at the three temperatures involved. Since all the slices at the start of incubation are in approximately the same metabolic state and the same ionic environment, variation of 1D with A1T should be at a minimum with a zero time approximation.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%