The effect of dietary magnesium and caseinphosphopeptides on bone metabolism in rats

Abstract

The effect of dietary magnesium (Mg) or caseinphosphopeptides (CPPs) on bone metabolism has been reported. However, few studies have investigated the effects of simultaneous supplementation of Mg and CPPs. Sixty-three 3-week-old Sprague-Dawley male rats were divided into seven groups and fed a specified diet for 45 days. Body characteristics, bone physicochemical indicators, and bone metabolism indicators relative to bone metabolism were analyzed. We found that, first, a dietary Mg deficiency resulted in increased bone formation and decreased bone resorption. Second, dietary Mg or CPP supplementation promoted bone formation and prevented bone resorption. Third, dietary Mg supplementation with CPPs also functioned to enhance bone formation and prevent bone resorption. There were synergistic effects on femur length, serum parathyroid hormone level and urinary deoxypyridinoline of the HS-Mg-CPP group (0.2% Mg, 0.1% CPPs). The increase in the femur length of the HS-Mg-CPP group compared with the control group was 6% which was much higher than that of HS-Mg (1%) or CPPs (5%). The induction in serum parathyroid hormone content in the HS-Mg-CPP group was 33% compared with the control group which was higher than that of the induction of the HS-Mg (19%) or CPP (23%) group. The induction in the deoxypyridinoline content of the HS-Mg-CPP (43%) group compared with the control group was remarkably higher than that of HS-Mg (8%) or CPPs (16%). Overall our results demonstrated that high doses of Mg (0.2%) and CPPs (0.1%) in combination produced synergistic effects on femur length, serum parathyroid hormone level and urinary deoxypyridinoline in rats, which is important for a better understanding of the effect of Mg and CPPs on bone metabolism.

Magnesium Picolinate Improves Bone Formation by Regulation of RANK/RANKL/OPG and BMP-2/Runx2 Signaling Pathways in High-Fat Fed Rats

“…Mineral supplements can be beneficial in preventing or treating bone diseases with bone loss [ 38 , 39 ]. For example, Liu et al [ 40 ] showed that Mg supplementation prevented bone resorption and increased bone formation in growing male rats [ 40 ]. Similarly, a meta-analysis study reported increased BMD (r 0.16, 95% CI 0.001) in humans associated with Mg consumption [ 41 ].…”

An 11-bp Indel Polymorphism within the CSN1S1 Gene Is Associated with Milk Performance and Body Measurement Traits in Chinese Goats

“…In addition, as caseins could promote bone formation and body development by increasing intestinal calcium absorption and bioavailability [14,[26][27][28]49], the effect of this 11-bp indel of the CSN1S1 gene on the body measurement traits of goats is worth studying. The association analysis results demonstrated that this polymorphism significantly affected the body measurement traits of goats and affected the different body measurement sizes in different goat breeds (Table 3; p < 0.05).…”

Recent advances in carbon dots: synthesis and applications in bone tissue engineering

“…It is well known that Mg 2+ can significantly affect the proliferation and differentiation of osteoblast cells by stimulating hormone secretion or cytokine synthesis. [88][89][90] Many reports have proved that Mg and Mgdoped implants can promote osteoblastic cell adhesion and bone regeneration. [91][92][93] In an in vitro assay, the Mg-CDs exhibited low cytotoxicity to mouse embryo osteoblast precursor cells (MC3T3-E1).…”