The effect of different morphological sampling criteria on the fraction of bursting cells recorded in the rat subiculum in vitro

Prida, Liset Menéndez de la; Suárez, Francisco; Pozo, M.A.

doi:10.1016/s0304-3940(02)00085-x

Cited by 11 publications

(7 citation statements)

References 14 publications

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“…This result was consistent with that obtained from previous in vitro studies (20,34,48). The firing rate of irregular discharge neurons under urethane anesthesia observed in our experiments was similar to that of the freely moving state (2) but was lower than that in slice preparation (19,38). An example of an irregular discharging neuron in SE mice is shown in Figure 5.…”

Section: Neurons With Irregular Dischargessupporting

confidence: 92%

“…However, there is a large discrepancy in the percentages of these three types of neurons between this report and previous reports. Previous in vitro studies showed that bursting activity is a vital characteristic of subicular pyramidal neurons (30, 35, 37, 48). Our results showed that in vivo spontaneous patterns are correlated with the immunochemical properties of Dsub neurons.…”

Section: Discussionmentioning

confidence: 99%

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Morpho‐Physiologic Characteristics of Dorsal Subicular Network in Mice after Pilocarpine‐Induced Status Epilepticus

Tang

et al. 2009

Brain Pathology

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The goal of this study was to examine morpho-physiological changes in the dorsal subiculum network in the mouse model of temporal lobe epilepsy using extracellular recording, juxtacellular and immunofluorescence double labeling, and anterograde tracing methods. A significant loss of total dorsal subicular neurons, particularly calbindin, parvalbumin (PV), and immunopositive interneurons, was found at 2 months after pilocarpine-induced status epilepticus (SE). However, the sprouting of axons from lateral entorhinal cortex (LEnt) was observed to contact with surviving subicular neurons. These neurons had two predominant discharge patterns: bursting and fast irregular discharges. The bursting neurons were mainly pyramidal cells, and their dendritic spine density and bursting discharge rates were increased significantly in SE mice compared to the control group. Fast irregular discharge neurons were PV-immunopositive interneurons, and had less dendritic spines in SE mice when compared to control mice. When LEnt was stimulated, bursting and fast irregular discharge neurons had much shorter latency and stronger excitatory response in SE mice compared to the control group. Our results illustrate that morpho-physiological changes in the dorsal subiculum could be part of a multilevel pathological network that occurs simultaneously in many brain areas to contribute to the generation of epileptiform activity.

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Section: Neurons With Irregular Dischargessupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Morpho‐Physiologic Characteristics of Dorsal Subicular Network in Mice after Pilocarpine‐Induced Status Epilepticus

Tang

et al. 2009

Brain Pathology

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“…Methods). Indeed, as previously evidenced by Menendez de La Prida [22], the different IB∶RS proportion is consequent to the sampling criteria.…”

Section: Discussionsupporting

confidence: 53%

Cell Type-Specific Properties of Subicular GABAergic Currents Shape Hippocampal Output Firing Mode

2012

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GABAergic function of the subiculum is central to the regulation of hippocampal output activity. Subicular neuronal networks are indeed under potent control by local inhibition. However, information about the properties of GABAergic currents generated by neurons of this parahippocampal area in normal tissue is still missing. Here, we describe GABAA receptor (GABAAR)-mediated phasic and tonic currents generated by principal cells (PCs) and interneurons (INs) of the rat subiculum. We show that in spite of similar synaptic current densities, INs generate spontaneous IPSCs (sIPSCs) that occur less frequently and exhibit smaller charge transfer, thus receiving less synaptic total current than PCs. Further distinction of PCs between intrinsically bursting (IB) and regular-spiking (RS) neurons suggested that sIPSCs generated by the two PC sub-types are likely to be similar. PCs and INs are also controlled by a similar tonic inhibition. However, whereas a comparable tonic current density is found in RS cells and INs, IB neurons are constrained by a greater inhibitory tone. Finally, pharmacological blockade of GABAAR did not promote functional switch of RS neurons to IB mode, but influenced the bursting propensity of IB cells and released fast spiking activity in INs. Our findings reveal differences in GABAergic currents between PCs and INs as well as within PC sub-types. We propose that GABAergic inhibition may shape hippocampal output activity by providing cell type-specific fine-tuning of subicular excitatory and inhibitory drives.

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“…Subicular cells were electrophysiologically classified as weak (IBϪ) and strong (IBϩ) bursting, regular-spiking (RS) and FS, from their responses to depolarizing current pulses of 500 msec duration (Stewart and Wong, 1993;Staff et al, 2000;Menendez de la Prida et al, 2002, 2003. Intrinsic properties were determined in Mg 2ϩ -free solution.…”

Section: Methodsmentioning

confidence: 99%

Synaptic Contributions to Focal and Widespread Spatiotemporal Dynamics in the Isolated Rat SubiculumIn Vitro

Prida¹,

Gal²

2004

J. Neurosci.

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The subiculum, which has a strategic position in controlling hippocampal activity, is receiving significant attention in epilepsy research. However, the functional organization of subicular circuits remains unknown. Here, we combined different recording and analytical methods to study focal and widespread population activity in the isolated subiculum in zero Mg 2ϩ media. Patch and field recordings were combined to examine the contribution of different cell types to population activity. The properties of cells leading field activity were examined. Predictive factors for a cell to behave as leader included exhibiting the bursting phenotype, displaying a low firing threshold, and having more distal apical dendrites. A subset of bursting cells constituted the first glutamatergic type that led a recruitment process that subsequently activated additional excitatory as well as inhibitory cells. This defined a sequence of synaptic excitation and inhibition that was studied by measuring the associated conductance changes and the evolution of the composite reversal potential. It is shown that inhibition was time-locked to excitation, which shunted excitatory inputs and suppressed firing during focal activity. This was recorded extracellularly as a multi-unit ensemble of active cells, the spatial boundaries of which were controlled by inhibition in contrast to widespread epileptiform activity. Focal activity was not dependent on the preparation or the developmental state because it was also recorded under 5 mM [K ϩ ] o and in adult tissue. Our data indicate that the subicular networks can be spontaneously organized as leader-follower local circuits in which excitation is mainly driven by a subset of bursting cells and inhibition controls spatiotemporal firing.

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The effect of different morphological sampling criteria on the fraction of bursting cells recorded in the rat subiculum in vitro

Cited by 11 publications

References 14 publications

Morpho‐Physiologic Characteristics of Dorsal Subicular Network in Mice after Pilocarpine‐Induced Status Epilepticus

Morpho‐Physiologic Characteristics of Dorsal Subicular Network in Mice after Pilocarpine‐Induced Status Epilepticus

Cell Type-Specific Properties of Subicular GABAergic Currents Shape Hippocampal Output Firing Mode

Synaptic Contributions to Focal and Widespread Spatiotemporal Dynamics in the Isolated Rat SubiculumIn Vitro

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