The Effect of Different Orthodontic Force on MMP 9 Expression in a Rat Diabetic Model

Abstract

Orthodontic forces produce alveolar bone and periodontal ligament remodeling that cause tooth movement. Diabetes can increase tissue damage and, therefore, contributes to the process of orthodontic tooth movement. The aim of this study was to determine the effect of differently orthodontic forces on the expression of MMP nine in rat diabetic model. Twenty four male Wistar rats were divided into three groups of control and three groups of treatment strept ozotocin-induced diabetes with stratified doses. Application of orthodontic appliance was performed with different forces (10, 20 and 30 grF). Orthodontic appliance was performed on both upper incisors. Immunohistochemical (IHC) analysis was used to observe the expression of MMP 9 and HE (Hematoxylen Eosin) staining to observe the number of fibroblasts. Statistical analysis was carried out using t -test and Spearman correlation analysis to determine the difference of MMP nine expression and number of fibroblasts between groups, and determine the correlation of both. The results showed an increased expression of MMP 9 and decreased of fibroblasts number in diabetic rats, along with increasing magnitude of orthodontic forces. The different orthodontic forces given to diabetic rats affect the expression of MMP 9 and the number of fibroblasts, in which the expression of MMP 9 increased along with the increase of orthodontic forces, both at pressure and tension sides..

Evidence on the effect of uncontrolled diabetes mellitus on orthodontic tooth movement. A systematic review with meta-analyses in pre-clinical in- vivo research

“…After electronic database and hand-searching, a total of 290 eligible articles were retrieved, with 19 papers left for full-text evaluation after duplicate removal, title and abstract screening. Finally, five articles were excluded for reasons related to our pre-defined eligibility criteria, leaving a number of 14 articles for inclusion in this systematic review (Figure 1) (Arita et al, 2016;Braga et al, 2011;Damanakis, 2018;Ferreira et al, 2018;Gomes et al, 2017Gomes et al, , 2018Li, Zhang, Wang, Feng, & Bi, 2010;Maulana, Hikmah, Shita, Permatasari, & Widyarti, 2014;Mena Laura et al, 2019;Plut et al, 2015;Santamaria-Jr et al, 2019;Sun et al, 2017;Villarino et al, 2011;Zhang, Li, & Bi, 2011).…”

“…All but one study (Braga et al, 2011), recruited rats as animal models and specifically Wistar rats or Sprague-Dawley species (Arita et al, 2016;Damanakis, 2018;Ferreira et al, 2018;Gomes et al, 2017Gomes et al, , 2018Li et al, 2010;Maulana et al, 2014;Mena Laura et al, 2019;Plut et al, 2015;Santamaria-Jr et al, 2019;Sun et al, 2017;Villarino et al, 2011;Zhang et al, 2011). Braga et al, ( 2011) (Braga et al, 2011) recruited mice.…”

Effect of Caffeic Acid Phenethyl Ester Provision on Fibroblast Growth Factor-2, Matrix Metalloproteinase-9 Expression, Osteoclast and Osteoblast Numbers during Experimental Tooth Movement in Wistar Rats (Rattus norvegicus)

“…Orthodontic force can induce oxidative stress in the periodontal ligament that activates MMP-9. 31 CAPE can reduce inflammation by inhibiting the activity of nuclear factor-kappa β (NF-kβ). 32 NF-kβ activation is induced by interleukin (IL)-1β and TNF-α as proinflammatory cytokine during OTM.…”

Profil Osteoblas Dan Osteoklas Tulang Alveolar Pada Model Tikus Diabetes Tahap Awal Dengan Aplikasigaya Ortodonti Yang Berbeda

“…Desain alat ortodonti yang aplikasikan pada hewan coba berupa kawatstainless steel 0,012 U (ClassOne Orthodontics, USA) dengan koil diameter 2 mm, dan panjang lengan kawat 10 mm. Ujung lengan kawat di pasang pada matrix band (Meba, Germany) berbentuk cincin dengan panjang 2 mm (Maulana et al, 2014…”