The Effect of Different Preservation Media and Temperatures on Sperm Quality and Dna Integrity in Mouse Cauda Spermatozoa

Abstract

BACKGROUND:Mouse sperm can be stored for long or short-time periods. Nevertheless long-term storage leds to significantly reduced sperm quality and fertility because of cryodamage. Thus, in the storage of semen in mice, it is necessary to focus on media and temperatures that gives good results in short-term storage. OBJECTIVE:To determine favorable media for short-term storage of mice spermatozoa by evaluating progressive motility, viability, membrane function integrity, acrosome integrity and fragmented DNA rates at various storage temperatures . MATERIALS AND METHODS: Mouse spermatozoa were collected from epididymides of mature CD1 males and s amples were stored at 24°C and 4°C for 60 h.RESULTS: Motility, viability and membrane function of mice spermatozoa were greatest when stored in KSOM media. Motility and viability were not different when stored at refrigerator or room temperature in KSOM compared to HTF or PBS mediums for 48 h, but were after 60 h . There was n't any significant variation in terms of acrosome integrity in different preservation conditions. Fragmented DNA rates were similar in fresh sperm with KSOM and HTF media, while there was higher damage in PBS medium at 60 h . Overall, sperm parameters were affected significantly by the time of storage and type of preservation medium, and PBS extender was not suitable for mice spermatozoa at room and refrigerated temperatures as it caused the lowest progressive motility, viability, membrane function integrity and the highest DNA damage . CONCLUSION: Mice spermatozoa stored in KSOM retained the best sperm quality parameters both 24°C and 4°C for the first 48 h.