The effect of dihydroquercetin on active and passive ion transport systems in plant vacuolar membrane

Nurminsky, V. N.; Озолина, Н. В.; Sapega, J. G.; Zheleznykh, A. O.; Pradedova, E. V.; Korzun, A. M.; Salyaev, R. K.

doi:10.1134/s1062359009010014

Cited by 3 publications

(5 citation statements)

References 11 publications

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“…In the presence of oxidants, the stability (mechanical strength) of biological membranes decreases, and antioxidant enzymes may decelerate or inhibit lipid peroxidation reactions, thus enhancing membrane stability. Furthermore, changes in membrane stability, such as those provided by ion channels, can stabilize membranes (Nurminsky et al 2009 ).…”

Section: Discussionmentioning

confidence: 99%

GST activity and membrane lipid saturation prevents mesotrione-induced cellular damage in Pantoea ananatis

et al. 2016

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Callisto®, containing the active ingredient mesotrione (2-[4-methylsulfonyl-2-nitrobenzoyl]1,3-cyclohenanedione), is a selective herbicide that controls weeds in corn crops and is a potential environmental contaminant. The objective of this work was to evaluate enzymatic and structural changes in Pantoea ananatis, a strain isolated from water, in response to exposure to this herbicide. Despite degradation of mesotrione, probably due a glutathione-S-transferase (GST) pathway in Pantoea ananatis, this herbicide induced oxidative stress by increasing hydrogen peroxide production. Thiol fragments, eventually produced after mesotrione degradation, could be involved in increased GST activity. Nevertheless, there was no peroxidation damage related to this production, as malondialdehyde (MDA) synthesis, which is due to lipid peroxidation, was highest in the controls, followed by the mesotrione- and Callisto®-treated cultures at log growth phase. Therefore, P. ananatis can tolerate and grow in the presence of the herbicide, probably due an efficient control of oxidative stress by a polymorphic catalase system. MDA rates depend on lipid saturation due to a pattern change to a higher level of saturation. These changes are likely related to the formation of GST-mesotrione conjugates and mesotrione degradation-specific metabolites and to the presence of cytotoxic adjuvants. These features may shift lipid membrane saturation, possibly providing a protective effect to bacteria through an increase in membrane impermeability. This response system in P. ananatis provides a novel model for bacterial herbicide tolerance and adaptation in the environment.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-016-0240-x) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

GST activity and membrane lipid saturation prevents mesotrione-induced cellular damage in Pantoea ananatis

et al. 2016

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“…These results suggested that ethephon application may increase dihydroquercetin (DHQ) production. DHQ is a strong antioxidant and increases the stability of the tonoplast membrane, on account of its antioxidant properties, and decreases membrane permeability by suppressing ion channels [ 61 ]. Therefore, DHQ may play a role in stabilization of lutoid membranes, which are particularly sensitive to osmotic stress.…”

Section: Resultsmentioning

confidence: 99%

“…Thus, lutoid stability influences latex flow [ 62 ]. However, inhibition of membrane oxidation by DHQ leads to activation of H + -ATPase [ 61 ]. In the present microarray analysis, upregulation of several genes encoding H + -ATPases was observed (Table 5 ).…”

Section: Resultsmentioning

confidence: 99%

“…In addition, changes in expression of genes that participate in flavonoids biosynthesis, especially genes associated with DHQ production, were observed. Given that DHQ is believed to contribute to stabilization of lutoid membranes [ 61 ], latex exudation would be prolonged, which may suppress coagulation of rubber particles. This series of molecular events may be involved in the increase in latex yield caused by ethylene stimulation.…”

Section: Discussionmentioning

confidence: 99%

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Transcriptome analysis of Pará rubber tree (H. brasiliensis) seedlings under ethylene stimulation

et al. 2021

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Background Natural rubber (cis-1,4-polyioprene, NR) is an indispensable industrial raw material obtained from the Pará rubber tree (H. brasiliensis). Natural rubber cannot be replaced by synthetic rubber compounds because of the superior resilience, elasticity, abrasion resistance, efficient heat dispersion, and impact resistance of NR. In NR production, latex is harvested by periodical tapping of the trunk bark. Ethylene enhances and prolongs latex flow and latex regeneration. Ethephon, which is an ethylene-releasing compound, applied to the trunk before tapping usually results in a 1.5- to 2-fold increase in latex yield. However, intense mechanical damage to bark tissues by excessive tapping and/or over-stimulation with ethephon induces severe oxidative stress in laticifer cells, which often causes tapping panel dryness (TPD) syndrome. To enhance NR production without causing TPD, an improved understanding of the molecular mechanism of the ethylene response in the Pará rubber tree is required. Therefore, we investigated gene expression in response to ethephon treatment using Pará rubber tree seedlings as a model system. Results After ethephon treatment, 3270 genes showed significant differences in expression compared with the mock treatment. Genes associated with carotenoids, flavonoids, and abscisic acid biosynthesis were significantly upregulated by ethephon treatment, which might contribute to an increase in latex flow. Genes associated with secondary cell wall formation were downregulated, which might be because of the reduced sugar supply. Given that sucrose is an important molecule for NR production, a trade-off may arise between NR production and cell wall formation for plant growth and for wound healing at the tapping panel. Conclusions Dynamic changes in gene expression occur specifically in response to ethephon treatment. Certain genes identified may potentially contribute to latex production or TPD suppression. These data provide valuable information to understand the mechanism of ethylene stimulation, and will contribute to improved management practices and/or molecular breeding to attain higher yields of latex from Pará rubber trees.

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“…Dihydroquercetin (DHQ, taxifolin), a member of the flavonoids family, is one of the most prominent dietary antioxidants and has great therapeutic potential [17]. It was shown, that DHQ had strong stabilizing effect on the membranes of isolated vacuoles of common beet [18]. In vitro bioassays showed that DHQ is a strong inhibitor of Fusarium growth and macrospore formation [19].…”

Section: Discussionmentioning

confidence: 99%

Effect of natural and synthetic growth stimulators on <i>in vitro</i> rooting and acclimatization of common ash (<i>Fraxinus excelsior</i> L.) microplants

Lebedev¹,

Schestibratov²

2013

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Application of growth stimulators can be especially effective on plantlets in vitro of tree species which are usually worse rooted and adapted in comparison with annual plants. In our work we evaluate effects of natural (dihydroquercetin, Zircon) and synthetic growth stimulators (Melafen, Fumar, Epin-Extra) on rooting and acclimatization of common ash (Fraxinus excelsior L.) microplants. The 0.05%-0.2% Zircon and 10 −5 % Melafen enhanced in vitro rooting by 29%-37% and 31%, respectively. Melafen also stimulated root formation faster compared to control plants. The dihydroquercetin concentration of 0.01% increased rooting by 24% and root number per shoot by 1.8 times. In vitro plants rooted on media supplemented with Melafen, Fumar and Zircon demonstrated enhanced ability to adapt to non-sterile conditions and accelerated growth. Two months after planting to the greenhouse, plants rooted on 0.01% dihydroquercetin were 45% taller than the control. Weekly spraying of plantlets with 0.02% Epin-Extra containing 24-epibrassinolid stimulated growth of uniform plants with large leaves. The obtained results support the use of growth stimulators for application in clonal micropropagation of common ash both for large-scale production of planting stock and for conservation of rare and valuable genotypes.

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The effect of dihydroquercetin on active and passive ion transport systems in plant vacuolar membrane

Cited by 3 publications

References 11 publications

GST activity and membrane lipid saturation prevents mesotrione-induced cellular damage in Pantoea ananatis

GST activity and membrane lipid saturation prevents mesotrione-induced cellular damage in Pantoea ananatis

Transcriptome analysis of Pará rubber tree (H. brasiliensis) seedlings under ethylene stimulation

Effect of natural and synthetic growth stimulators on <i>in vitro</i> rooting and acclimatization of common ash (<i>Fraxinus excelsior</i> L.) microplants

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The effect of dihydroquercetin on active and passive ion transport systems in plant vacuolar membrane

Cited by 3 publications

References 11 publications

GST activity and membrane lipid saturation prevents mesotrione-induced cellular damage in Pantoea ananatis

GST activity and membrane lipid saturation prevents mesotrione-induced cellular damage in Pantoea ananatis

Transcriptome analysis of Pará rubber tree (H. brasiliensis) seedlings under ethylene stimulation

Effect of natural and synthetic growth stimulators on &lt;i&gt;in vitro&lt;/i&gt; rooting and acclimatization of common ash (&lt;i&gt;Fraxinus excelsior&lt;/i&gt; L.) microplants

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Effect of natural and synthetic growth stimulators on <i>in vitro</i> rooting and acclimatization of common ash (<i>Fraxinus excelsior</i> L.) microplants