Reduced activation martensitic steel is the main candidate structural material for key components of advanced nuclear energy systems because of its good mechanical properties at room temperature, low neutron activation characteristics and satisfactory radiation resistance. In this work, we studied the interaction between dislocations and helium-irradiation-induced defects in the steel and the effect of dislocations on the behavior of helium migration and desorption. The well-annealed samples were pre-deformed to 10% and 20% reductions in thickness by using cold rolling mill. After pre-deformation, samples were heat-treated to remove vacancy defects and retain dislocation defects. Then, the samples with reserved dislocations were irradiated with helium at room temperature (50 keV, 1×10<sup>17</sup> He/cm<sup>2</sup>). After irradiation, the samples were characterized by synchrotron radiation grazing incident X-Ray diffraction, positron annihilation doppler broadening spectroscopy and thermal desorption spectroscopy. The results show that dislocations hinder the diffusion of helium and helium-vacancy complexes, and reduce the accumulation of radiation damage. Such effect becomes more significant with the increase of dislocation density. The BCC → FCC phase transition of low activation martensitic steel occurs at 1179 K. The increase of dislocation density will lead to the forward shift of helium desorption peak induced by phase transition. The retention of helium in the undeformed sample, 10 % deformed sample and 20 % deformed sample was 10.3 %, 15.7 % and 17.9 %, indicating that high density dislocations promote the retention of helium.