The Effect of Dissolved Oxygen on Kinetics during Continuous Biocatalytic Oxidations

Lindeque, Rowan Malan; Woodley, John M.

doi:10.1021/acs.oprd.0c00140

Cited by 32 publications

(53 citation statements)

References 82 publications

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“…The generation of air containing fine bubbles through porous spargers, which are capable of generating microbubbles, are in the focus of this contribution. The mass transfer of fine bubbles as well as macrobubble aeration could further be enhanced by aeration with pure oxygen or enriched air (Lee, 2020; Lindeque & Woodley, 2020). Fine bubbles are successfully applied for the defouling of various surfaces, cleaning of sanitary facilities and in flotation processes (Tao, 2005; Wang, Wang, Yan, Wang, & Cao, 2017), and wastewater treatment (Wang et al, 2019; Khuntia, Majumder, & Ghosh, 2012; Temesgen, Bui, Han, Kim, & Park, 2017).…”

Section: Introductionmentioning

confidence: 99%

Comparative investigation of fine bubble and macrobubble aeration on gas utility and biotransformation productivity

Thomas

Ohde

Matthes

et al. 2020

Biotech & Bioengineering

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The sufficient provision of oxygen is mandatory for enzymatic oxidations in aqueous solution, however, in process optimization this still is a bottleneck that cannot be overcome with the established methods of macrobubble aeration. Providing higher mass transfer performance through microbubble aerators, inefficient aeration can be overcome or improved. Investigating the mass transport performance in a model protein solution, the microbubble aeration results in higher k L a values related to the applied airstream in comparison with macrobubble aeration. Comparing the aerators at identical k L a of 160 and 60 1/h, the microbubble aeration is resulting in 25 and 44 times enhanced gas utility compared with aeration with macrobubbles. To prove the feasibility of microbubbles in biocatalysis, the productivity of a glucose oxidase catalyzed biotransformation is compared with macrobubble aeration as well as the gas-saving potential. In contrast to the expectation that the same productivities are achieved at identically applied k L a, microbubble aeration increased the gluconic acid productivity by 32% and resulted in 41.6 times higher oxygen utilization. The observed advantages of microbubble aeration are based on the large volume-specific interfacial area combined with a prolonged residence time, which results in a high mass transfer performance, less enzyme deactivation by foam formation, and reduced gas consumption. This makes microbubble aerators favorable for application in biocatalysis.

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Section: Introductionmentioning

confidence: 99%

Comparative investigation of fine bubble and macrobubble aeration on gas utility and biotransformation productivity

Thomas

Ohde

Matthes

et al. 2020

Biotech & Bioengineering

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“…We utilized large pore size resins to maximize oxygen mass transfer and access of non-immobilized auxiliary enzymes. 26…”

Section: Resultsmentioning

confidence: 99%

Data-rich process development of immobilized biocatalysts in flow

Forstater

Grosser

2022

React. Chem. Eng.

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“…Furthermore, the reaction rate of biocatalytic oxidations, which relies on poorly water-soluble molecular oxygen gas, could be remarkably improved by performing the reactions in pressurized continuous flow systems. As previously mentioned, pressurized systems have been helpful in increasing the concentration of a dissolved gas, and studies showed that a threefold increase in the oxygen content positively affected the enzyme efficacy [74]. Besides singlestep transformations, continuous-flow systems can facilitate the performance of enzymatic cascade reactions by combining more reactors in series.…”

Section: Fundamentals Of Flow Biocatalysismentioning

confidence: 99%

Flow Biocatalysis: A Challenging Alternative for the Synthesis of APIs and Natural Compounds

Santi

Sancineto

Nascimento

et al. 2021

IJMS

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Biocatalysts represent an efficient, highly selective and greener alternative to metal catalysts in both industry and academia. In the last two decades, the interest in biocatalytic transformations has increased due to an urgent need for more sustainable industrial processes that comply with the principles of green chemistry. Thanks to the recent advances in biotechnologies, protein engineering and the Nobel prize awarded concept of direct enzymatic evolution, the synthetic enzymatic toolbox has expanded significantly. In particular, the implementation of biocatalysts in continuous flow systems has attracted much attention, especially from industry. The advantages of flow chemistry enable biosynthesis to overcome well-known limitations of “classic” enzymatic catalysis, such as time-consuming work-ups and enzyme inhibition, as well as difficult scale-up and process intensifications. Moreover, continuous flow biocatalysis provides access to practical, economical and more sustainable synthetic pathways, an important aspect for the future of pharmaceutical companies if they want to compete in the market while complying with European Medicines Agency (EMA), Food and Drug Administration (FDA) and green chemistry requirements. This review focuses on the most recent advances in the use of flow biocatalysis for the synthesis of active pharmaceutical ingredients (APIs), pharmaceuticals and natural products, and the advantages and limitations are discussed.

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The Effect of Dissolved Oxygen on Kinetics during Continuous Biocatalytic Oxidations

Cited by 32 publications

References 82 publications

Comparative investigation of fine bubble and macrobubble aeration on gas utility and biotransformation productivity

Comparative investigation of fine bubble and macrobubble aeration on gas utility and biotransformation productivity

Data-rich process development of immobilized biocatalysts in flow

Flow Biocatalysis: A Challenging Alternative for the Synthesis of APIs and Natural Compounds

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