The Effect of Dmso on Several Enzyme Systems*

Rammler, D. H.

doi:10.1111/j.1749-6632.1967.tb34893.x

Cited by 137 publications

(40 citation statements)

References 16 publications

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“…9). The reduced performance of the cells pre-treated with (Rammler 1967). However, the difference between the solid application of substrate to the cells pretreated with DMSO and the conventional batch process can most likely be explained by inhibition by the substrate and product present in the aqueous phase when the co-solvent is present.…”

Section: Substrate Supplymentioning

confidence: 92%

Process limitations of a whole-cell P450 catalyzed reaction using a CYP153A-CPR fusion construct expressed in Escherichia coli

Lundemo

Notonier

Striedner

et al. 2015

Appl Microbiol Biotechnol

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Cytochrome P450s are interesting biocatalysts due to their ability to hydroxylate non-activated hydrocarbons in a selective manner. However, to date only a few P450-catalyzed processes have been implemented in industry due to the difficulty of developing economically feasible processes. In this study, we have used the CYP153A heme domain from Marinobacter aquaeolei fused to the reductase domain of CYP102A1 from Bacillus megaterium (BM3) expressed in Escherichia coli. This self-sufficient protein chimera CYP153A-CPRBM3 G307A mutant is able to selectively hydroxylate medium and long chain length fatty acids at the terminal position. ω-Hydroxylated fatty acids can be used in the field of high-end polymers and in the cosmetic and fragrance industry. Here, we have identified the limitations for implementation of a whole-cell P450-catalyzed reaction by characterizing the chosen biocatalyst as well as the reaction system. Despite a well-studied whole-cell P450 catalyst, low activity and poor stability of the artificial fusion construct are the main identified limitations to reach sufficient biocatalyst yield (mass of product/mass of biocatalyst) and space-time yield (volumetric productivity) essential for an economically feasible process. Substrate and product inhibition are also challenges that need to be addressed, and the application of solid substrate is shown to be a promising option to improve the process.

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Section: Substrate Supplymentioning

confidence: 92%

Process limitations of a whole-cell P450 catalyzed reaction using a CYP153A-CPR fusion construct expressed in Escherichia coli

Lundemo

Notonier

Striedner

et al. 2015

Appl Microbiol Biotechnol

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“…DMSO may affect enzymatic systems in a variety of ways based on altering the catalysis reaction of the enzyme or by changing the enzyme protein, the substrate products, or co-enzymes. It is assumed that these alterations take place because of the conformational alterations that result by substitution of DMSO for protein-bound water [16], In all enzyme systems tested by R ammler the loss of enzymatic action was reversible des pite prolonged periods of administration [15].…”

Section: Discussionmentioning

confidence: 99%

Effect of Dimethyl Sulfoxide (DMSO) on Gastric Secretion in Rats

Bralow¹,

Gruenstein²,

Hitanant³

et al. 1973

Digestion

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Gastric secretory studies using a 4-hour pylorusligation technique in rats were performed after using dimethyl sulfoxide (DMSO) as a solvent for gastric carcinogens Single intragastric doses of 1 ml 50% DMSO reduced the 4-hour volume (vol) by 48%, titratable acidity (TA) by 20%, and acid output (TAO) by 57.3%. Pepsin was not affected. With 1 ml 100% DMSO, gastric secretion was mostly mucus and vol was unchanged, TA reduced 87.4%, TAO 91%, and pepsin 66%. Subcutaneous injections of 1 ml DMSO in varying concentrations inhibited gastric secretion in a linear fashion; vol (r = 0.605) and TAO (r = 0.626). TA was not decreased unless concentration of DMSO was greater than 50%. Pepsin was not affected. Concomitant decreases in succinic dehydrogenase activity were noted by histochemical techniques.

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“…Cryopreservatives are known to be toxic to cells [3]. Since dimethyl sulfoxide is considered to be toxic on the basis of its effects on intracellular enzymes and cell membranes [8,9] we use the trypan blue dye exclusion assay for the determination of viability after thwaing. This test is based on the fact that cells will be able to exclude the dye if the cell membrane is intact.…”

Section: Discussionmentioning

confidence: 99%

Culture and Cryopreservation of Chondrocytes from Human Cartilage: Relevance for Cartilage Allografting in Otolaryngology

Bujía

Pitzke²,

Wilmes³

et al. 1992

ORL

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One of the reasons for failure of cartilage allografts is the impaired condition of the transplant during storage. In this paper we describe methods for the isolation and culture of viable chondrocytes obtained from nasal septum cartilage. Furthermore, we evaluate the possibility of growing such specific chondrocytes under culture conditions and storing them in a frozen state. Age dependent differences were observed in the growth rate of the cultured cells. Our results confirm that chondrocytes survive freezing and remain able to proliferate. Knowledge gained from this study may be applied to the culture and freezing of viable intact cartilage for use in reconstructive surgery in otolaryngology.

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The Effect of Dmso on Several Enzyme Systems*

Cited by 137 publications

References 16 publications

Process limitations of a whole-cell P450 catalyzed reaction using a CYP153A-CPR fusion construct expressed in Escherichia coli

Process limitations of a whole-cell P450 catalyzed reaction using a CYP153A-CPR fusion construct expressed in Escherichia coli

Effect of Dimethyl Sulfoxide (DMSO) on Gastric Secretion in Rats

Culture and Cryopreservation of Chondrocytes from Human Cartilage: Relevance for Cartilage Allografting in Otolaryngology

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