The effect of dopamine on lung liquid production by <i>in vitro</i> lungs from fetal guinea‐pigs

Chua, Beverly; Perks, A. M.

doi:10.1111/j.1469-7793.1998.283by.x

Cited by 20 publications

(19 citation statements)

References 34 publications

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“…In isolated adult rat lungs, SCH 23390, but neither S(-) sulpiride nor propranolol inhibited DAs effect suggesting the D1 DA receptor pathway is involved (29). In contrast, increased lung liquid absorption was mediated through D2 receptors in fetal guinea pig lung after a short-term exposure to DA (ϳ1 h) (10). Our experiments showing that the DAinduced increase in Isc can be blocked with D1/D2 receptor blockers are consistent with these reports.…”

Section: Discussionsupporting

confidence: 82%

“…Studies in isolated adult rat lungs have demonstrated that DA stimulates lung fluid absorption (10,29). However, there is some controversy regarding the specific mechanisms involved.…”

Section: Discussionmentioning

confidence: 99%

“…They secrete many mediators, including dopamine (DA), serotonin, gastrin-releasing peptide (GRP), and somatostatin, (7)(8)(9) which may play a role in the lungs' transition to postnatal life by altering fetal lung epithelial ion and fluid transport. DA concentrations are maximal at birth (10), and it is known to alter alveolar fluid transport by increasing Na ϩ channel and Na ϩ /K ϩ -ATPase activity (10,11). Some studies suggest that serotonin may have a similar role in fluid clearance in the fetal lung (12); however, others suggest that serotonin decreases transepithelial Na ϩ transport (13).…”

mentioning

confidence: 99%

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Pulmonary Neuroendocrine Cell-Secreted Factors May Alter Fetal Lung Liquid Clearance

et al. 2009

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ABSTRACT:The neuroendocrine system is most active at birth and may play a role in the transition from fetal to postnatal life, in particular in the lungs' transition from fluid secretion to fluid absorption. Pulmonary neuroendocrine cells do release dopamine (DA), serotonin, and gastrin-releasing peptide but their effects on lung ion and fluid transport are poorly understood. Therefore, we studied their effects on fetal distal lung explants and primary cultures of fetal distal lung epithelium (FDLE). We show that DA, but neither serotonin nor gastrin-releasing peptide, alters ion and fluid transport, in a dosedependent manner. DAs effects were abrogated by D1/D2 receptor blockers in FDLE but not in explants. Propranolol abrogated DAs effects in both models. DA increased intracellular cAMP levels in FDLE. Terbutaline, forskolin, and isobutylmethylxanthine did not increase short circuit current (Isc) in DA-treated cells, despite a further increase in cAMP. We conclude that at least one, but not all mediators released by pulmonary neuroendocrine cells alter distal lung epithelial ion transport. (Pediatr Res 65: 274-278, 2009)

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Section: Discussionsupporting

confidence: 82%

“…Studies in isolated adult rat lungs have demonstrated that DA stimulates lung fluid absorption (10,29). However, there is some controversy regarding the specific mechanisms involved.…”

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Pulmonary Neuroendocrine Cell-Secreted Factors May Alter Fetal Lung Liquid Clearance

et al. 2009

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“…This limited access we presume is a result of only the smaller molecules of the population being capable of entering the matrix pores. In other studies, larger objects (2,000kDa dextrans, R S = ~27nm; and type-5 adenovirus vectors, R S = ~49nm) were shown to pass through the lens capsule in a two chamber diffusion system (62) or after anterior chamber injection (63,64). Considering the maximum pore size we have measured in the capsule, this contradiction may suggest that the capsules used in the previous reports were compromised.…”

Section: Discussionmentioning

confidence: 91%

Characterizing molecular diffusion in the lens capsule

et al. 2010

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The lens capsule compartmentalizes the cells of the avascular lens from other ocular tissues. Small molecules required for lens cell metabolism, such as glucose, salts, and waste products, freely pass through the capsule. However, the lens capsule is selectively permeable to proteins such as growth hormones and substrate carriers which are required for proper lens growth and development. We used fluorescence recovery after photobleaching (FRAP) to characterize the diffusional behavior of various sized dextrans (3, 10, 40, 150, and 250kDa) and proteins endogenous to the lens environment (EGF, γD-crystallin, BSA, transferrin, ceruloplasmin, and IgG) within the capsules of whole living lenses. We found that proteins had dramatically different diffusion and partition coefficients as well as capsule matrix binding affinities than similar sized dextrans, but they had comparable permeabilities. We also found ionic interactions between proteins and the capsule matrix significantly influence permeability and binding affinity, while hydrophobic interactions had less of an effect. The removal of a single anionic residue from the surface of a protein, γD-crystallin [E107A], significantly altered its permeability and matrix binding affinity in the capsule. Our data indicated that permeabilities and binding affinities in the lens capsule varied between individual proteins and cannot be predicted by isoelectric points or molecular size alone.

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“…In addition, previous experiments in ATII (33) have demonstrated that they do not contribute to net transepithelial Na ϩ transport. Two studies have reported that dopamine-induced lung fluid clearance is amiloride insensitive (4,9), but a more recent study suggests a role of dopamine in ENaC regulation (18). The CNGC Na ϩ -permeant ion channel has been reported to be expressed within the lung and to be relatively insensitive to amiloride (12).…”

Section: Discussionmentioning

confidence: 97%

Effects of cardiogenic edema fluid on ion and fluid transport in the adult lung

Gandhi¹,

Rafii²,

Harris³

et al. 2007

American Journal of Physiology-Lung Cellular and Molecular Phys

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We have previously shown that cardiogenic pulmonary edema fluid (EF) increases Na(+) and fluid transport by fetal distal lung epithelia (FDLE) (Rafii B, Gillie DJ, Sulowski C, Hannam V, Cheung T, Otulakowski G, Barker PM and O'Brodovich H. J Physiol 544: 537-548, 2002). We now report the effect of EF on Na(+) and fluid transport by the adult lung. We first studied primary cultures of adult type II (ATII) epithelium and found that overnight exposure to EF increased Na(+) transport, and this effect was mainly due to factors other than catecholamines. Plasma did not stimulate Na(+) transport in ATII. Purification of EF demonstrated that at least some agent(s) responsible for the amiloride-insensitive component resided within the globulin fraction. ATII exposed to globulins demonstrated a conversion of amiloride-sensitive short-circuit current (I(sc)) to amiloride-insensitive I(sc) with no increase in total I(sc). Patch-clamp studies showed that ATII exposed to EF for 18 h had increased the number of highly selective Na(+) channels in their apical membrane. In situ acute exposure to EF increased the open probability of Na(+)-permeant ion channels in ATII within rat lung slices. EF did increase, by amiloride-sensitive pathways, the alveolar fluid clearance from the lungs of adult rats. We conclude that cardiogenic EF increases Na(+) transport by adult lung epithelia in primary cell culture, in situ and in vivo.

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The effect of dopamine on lung liquid production by in vitro lungs from fetal guinea‐pigs

Cited by 20 publications

References 34 publications

Pulmonary Neuroendocrine Cell-Secreted Factors May Alter Fetal Lung Liquid Clearance

Pulmonary Neuroendocrine Cell-Secreted Factors May Alter Fetal Lung Liquid Clearance

Characterizing molecular diffusion in the lens capsule

Effects of cardiogenic edema fluid on ion and fluid transport in the adult lung

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