The effect of duodenal infusion of histidine on milk yield, milk composition, and plasma amino acids in dairy cows

Abstract

The objective of this study was to evaluate the effect of duodenal infusion of histidine on milk yield, milk composition, and plasma amino acids in lactating dairy cows. In the present experiment, three high-yield lactating Holstein cows fitted with duodenal closed T-shaped cannulas were used. The cows were allocated to two treatments: histidine deficient (control) and histidine supplemented (HIS). The experiment was divided into four periods of 7 d. Each period consisted of a 3-d preliminary period and a 4-d experimental period. A crossover design was used for the experiment. The cows were fed ad libitum a diet consisting of maize silage, lucerne hay, and a supplement mix. Dry matter intake did not differ (P>0.05) between treatments. Milk yield and the yields of protein, casein, and lactose were significantly higher in HIS in comparison with the control. The contents of protein, casein, fat, and lactose were not significantly different (P>0.05). The proportions of α-casein and β-casein did not show significant variations (P>0.05), but the κ-casein proportion was significantly higher (P<0.01) in HIS. The yield of each casein fraction in HIS was significantly higher compared with the control. No effect of duodenal infusion of histidine on the concentration of free amino acids in blood plasma was observed (P>0.05) except for histidine (P=0.012).

Histidine dose-response effects on lactational performance and plasma amino acid concentrations in lactating dairy cows: 1. Metabolizable protein-adequate diet

“…However, there was no effect of L-His infusion on ECM in either experiment. A short-term duodenal infusion of 13.6 g/d of His increased MY by 1.1 kg and ECM yield by 0.9 kg (Hadrová et al, 2012). Intravenous infusion of increasing doses of L-His to cows fed grass silagebased diets also increased MY quadratically by 3.4 kg/d (no His infusion vs. 6 g/d infused His), or linearly from 25.9 (no His) to 28.4 kg/d (9 g/d infused His) when infused together with Met and Lys (Kim et al, 2001).…”

Histidine: A Systematic Review on Metabolism and Physiological Effects in Human and Different Animal Species

“…S. Hadrova et al [115] evaluated the effect of histidine on milk yield and milk composition on high-yield, lactating Holstein cows. The cows were fed with a histidine deficient diet (18% deficiency) and with a diet supplemented in histidine at 13.6 g/d.…”

Short communication: Relationship between lysine/methionine ratios and glucose levels and their effects on casein synthesis via activation of the mechanistic target of rapamycin signaling pathway in bovine mammary epithelial cells

“…Of all the combinations of Lys and Met levels evaluated by Nan et al (2014), the greatest casein concentration in culture medium was obtained at 1.2 mM Lys and 0.4 mM Met, whereas the smallest casein concentration was obtained at 1.4 mM Lys and 0.6 mM Met. The concentration of Lys used in this work ranged from 5-to 20-fold and the concentration of Met ranged from 3-to 24-fold compared with the concentration of Lys and Met in arterial plasma in dairy cows (Hadrová et al, 2012;Nichols et al, 2016). The glucose level was more than 3-fold greater than the concentrations in the arterial blood of the dairy cow (Rius et al, 2010) and was the recommended dose in high-glucose DMEM/F-12 for cell cul-ture from Gibco (Waltham, MA).…”