The effect of EDTA contaminated in sera on laboratory data

Imafuku, Yuji; Meguro, Sakiko; Kanno, Ken‐ichiro; Hiraki, Hiroyuki; Nemoto, Utako; Hata, Ryoko; Takahashi, Kiyoaki; Miura, Yutaka; Yoshida, Hiroshi

doi:10.1016/s0009-8981(02)00251-6

Cited by 21 publications

(20 citation statements)

References 5 publications

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“…2,3 In our study, EDTA contamination was limited to samples from inpatients and general practice. It is interesting to note that EDTA contamination was not evident in samples from outpatients, which accounts for 20% of our workload and where blood samples are collected by trained phlebotomists.…”

Section: Discussionmentioning

confidence: 99%

Spurious hyperkalaemia due to EDTA contamination: common and not always easy to identify

2008

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Background: To study the detection and prevalence of spurious hyperkalaemia due to potassium ethylenediaminetetra-acetic acid (kEDTA) contamination. Methods: In a one-month prospective study, serum EDTA, zinc, calcium, magnesium concentrations and alkaline phosphatase activity were measured in samples with serum potassium 6.0 mmol/L. Results: Twenty-eight out of 117 hyperkalaemic samples were contaminated with EDTA. Only serum zinc values below the reference range had 100% sensitivity for indicating EDTA contamination, but even at an optimal specificity of 89% at least 12 potentially genuine hyperkalaemic samples would be rejected. Conclusion: Spurious hyperkalaemia due to kEDTA contamination is common. Gross kEDTA contamination is obvious by marked unexpected hyperkalaemia, hypocalcaemia, hypomagnesaemia and hypozincaemia. Spurious hyperkalaemia due to low concentrations of kEDTA contamination can only be confidently detected by measurement of serum EDTA.

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Section: Discussionmentioning

confidence: 99%

Spurious hyperkalaemia due to EDTA contamination: common and not always easy to identify

2008

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“…A negative iron value is usually due to EDTA contamination of sera, which can be suspected on finding low calcium and high potassium concentrations. 6 However, in our case, serum calcium and potassium levels were normal, so we ruled out the possibility of EDTA contamination. We were extremely satisfied to uncover a hitherto undiagnosed multiple myeloma in this patient.…”

Section: Multiple Myeloma Uncovered By Investigating a Negative Serummentioning

confidence: 49%

Anaplastic large cell lymphoma presenting with paraneoplastic pemphigus

Davis¹,

Cole‐Sinclair

Russell

2007

J Clin Pathol

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“…Several studies have shown that TIMP-1 blood levels are dependent on preanalytical conditions [17][18][19][20][21][22] . During our own investigations on the clinical use of TIMP-1 we made some unexpected observations that likely could only be explained by a possible bias of different specimen handling used.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, TIMP-1 plasma levels seem to play an important role in metabolic disorders, such as diabetes and adiposity [8][9][10] , as well as in patients with cardiac disease [11][12][13][14][15] and sepsis [16] . In addition to these promising investigations, some authors have presented results indicating that TIMP-1 levels differ depending on pre-and perianalytical conditions [17][18][19][20][21][22] . Higher levels of TIMP-1 have been measured in serum than in plasma, which raised suspicion that TIMP-1 is released by leukocytes and platelets during the coagulation process and therefore plasma has been suggested to be the appropriate medium to measure TIMP-1 levels in peripheral blood [17,[19][20][21] .…”

Section: Introductionmentioning

confidence: 99%

Dependence of TIMP-1 Plasma Levels on Preanalytical Specimen Handling

Dresse¹,

Nagel²,

Ganser³

et al. 2008

Tumor Biol

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Background: Tissue inhibitor of metalloproteinases-1 (TIMP-1) in blood might be a helpful biomarker in various diseases. However, various authors report that TIMP-1 is dependent on preanalytical procedures. Our study was performed to determine how storage conditions and time to centrifugation influence TIMP-1. Materials and Methods: Twenty-six blood specimens were collected from each of 20 volunteers. Two specimens from each person were centrifuged/measured within 1 h after venipuncture and frozen at –80°C. They were thawed once or twice within 72 h. Eight specimens were stored at 20°C in daylight, 8 at 20°C covered and 8 at 4°C in daylight. Four of each of these 8 specimens were mixed once a day until centrifugation. A mixed and an unmixed specimen of each group was centrifuged/measured after 3, 6, 24 and 72 h. Results: TIMP-1 increased after freeze/thaw (p < 0.001). Mixing blood specimens more than once caused increased TIMP-1 (p < 0.001). TIMP-1 increased within 3 h of storage (p < 0.001). The increase was lower in specimens covered and refrigerated (p < 0.001). Conclusion: TIMP-1 is unstable and has to be evaluated carefully. Blood should be centrifuged directly after venipuncture. For routine application, specimen handling must be standardized and carefully followed. Research should be done on specimens handled identically.

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The effect of EDTA contaminated in sera on laboratory data

Cited by 21 publications

References 5 publications

Spurious hyperkalaemia due to EDTA contamination: common and not always easy to identify

Spurious hyperkalaemia due to EDTA contamination: common and not always easy to identify

Anaplastic large cell lymphoma presenting with paraneoplastic pemphigus

Dependence of TIMP-1 Plasma Levels on Preanalytical Specimen Handling

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