The effect of elastic biodegradable polyurethane electrospun nanofibers on the differentiation of mesenchymal stem cells

Kuo, Yuh‐Chen; Hung, Shih‐Chieh; Hsu, Shan‐hui

doi:10.1016/j.colsurfb.2014.07.017

Cited by 56 publications

(32 citation statements)

References 41 publications

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“…These results fit well with previous in vitro cytocompatibility tests carried out on PU fibers (Kuo et al, 2014). It was previously reported that MSCs can differentiate into cardiomyocyte-like cells after 5-azacytidine treatment (Antonitsis et al, 2007;Zhang et al, 2009;Li et al, 2013; Supokawej et al, 2013;Wu et al, 2013;Piryaei et al, 2015).…”

Section: Discussionsupporting

confidence: 81%

Cardiac patch design: compatibility of nanofiber materials prepared byelectrospinning method with stem cells

Saltik¹,

Öteyaka²

2016

Turk J Biol

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Section: Discussionsupporting

confidence: 81%

Cardiac patch design: compatibility of nanofiber materials prepared byelectrospinning method with stem cells

Saltik¹,

Öteyaka²

2016

Turk J Biol

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“…Kuo et al . reported that larger fibers (1.47 µm, compared to 0.67 and 0.31 µm) increased proliferation of hMSCs on electrospun polyurethane scaffolds [59]. They attribute enhanced proliferation on larger fibers to enhanced migration of cells into pores within the nonwoven scaffold [59].…”

Section: Discussionmentioning

confidence: 99%

Fabrication of novel high surface area mushroom gilled fibers and their effects on human adipose derived stem cells under pulsatile fluid flow for tissue engineering applications

2016

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The fabrication and characterization of novel high surface area hollow gilled fiber tissue engineering scaffolds via industrially relevant, scalable, repeatable, high speed, and economical nonwoven carding technology is described. Scaffolds were validated as tissue engineering scaffolds using human adipose derived stem cells (hASC) exposed to pulsatile fluid flow (PFF). The effects of fiber morphology on the proliferation and viability of hASC, as well as effects of varied magnitudes of shear stress applied via PFF on the expression of the early osteogenic gene marker runt related transcription factor 2 (RUNX2) were evaluated. Gilled fiber scaffolds led to a significant increase in proliferation of hASC after seven days in static culture, and exhibited fewer dead cells compared to pure PLA round fiber controls. Further, hASC-seeded scaffolds exposed to 3 and 6 dyne/cm2 resulted in significantly increased mRNA expression of RUNX2 after one hour of PFF in the absence of soluble osteogenic induction factors. This is the first study to describe a method for the fabrication of high surface area gilled fibers and scaffolds. The scalable manufacturing process and potential fabrication across multiple nonwoven and woven platforms makes them promising candidates for a variety of applications that require high surface area fibrous materials.

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“…These scaffolds can stimulate osteoconduction and osteoinduction, provide a fertile ground for stem cell proliferation, and be made out of biodegradable synthetic polymers that trigger little immunogenic response. 113,114 Indeed, inflammation and adhesion of macrophages, with formation/release of bioreactive agents such as reactive oxygen intermediates, digestive enzymes, and acidic phagolysosomes, may result in premature degradation of scaffolds. Control and manipulation of this response to enhance bone repair and regeneration has therefore been a goal of immunobioengineering, and some progress has been made through electrospun nanofibers which may minimize host response.…”

Section: Nano-based Scaffold Construction and Modificationmentioning

confidence: 99%

“…There have been new techniques to overcome the hydrophobic nature of polymeric materials using surfactants, 113 new scaffolds tailored to specific application such as aligned nanoyarn reinforcement for tendon repair, 115 new structural formations like that of cotton wool, 116 new composite combinations like chitosan (CS) and silk fibroin (SF), 117 and new confirmatory studies of the bone stimulating effects of known pro-osteogenic proteins like BMP-2 within the context of electrospun scaffolds. 118 Electrospun scaffolds are typically limited for bone tissue replacement due to low mechanical strength.…”

Section: Nano-based Scaffold Construction and Modificationmentioning

confidence: 99%

Nanotechnology in bone tissue engineering

Walmsley

McArdle

Tevlin

et al. 2015

Nanomedicine: Nanotechnology, Biology and Medicine

239

162

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Nanotechnology represents a major frontier with potential to significantly advance the field of bone tissue engineering. Current limitations in regenerative strategies include impaired cellular proliferation and differentiation, insufficient mechanical strength of scaffolds, and inadequate production of extrinsic factors necessary for efficient osteogenesis. Here we review several major areas of research in nanotechnology with potential implications in bone regeneration: 1) nanoparticle-based methods for delivery of bioactive molecules, growth factors, and genetic material, 2) nanoparticle-mediated cell labeling and targeting, and 3) nano-based scaffold construction and modification to enhance physicochemical interactions, biocompatibility, mechanical stability, and cellular attachment/survival. As these technologies continue to evolve, ultimate translation to the clinical environment may allow for improved therapeutic outcomes in patients with large bone deficits and osteodegenerative diseases.

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The effect of elastic biodegradable polyurethane electrospun nanofibers on the differentiation of mesenchymal stem cells

Cited by 56 publications

References 41 publications

Cardiac patch design: compatibility of nanofiber materials prepared byelectrospinning method with stem cells

Cardiac patch design: compatibility of nanofiber materials prepared byelectrospinning method with stem cells

Fabrication of novel high surface area mushroom gilled fibers and their effects on human adipose derived stem cells under pulsatile fluid flow for tissue engineering applications

Nanotechnology in bone tissue engineering

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