The effect of ellagic acid on spinal cord and sciatica function in a mice model of multiple sclerosis

Khodaei, Forouzan; Khoshnoud, Mohammad Javad; Heidaryfar, Sepideh; Heidari, Reza; Baseri, Mohammad H. Karimpour; Azarpira, Negar; Rashedinia, Marzieh

doi:10.1002/jbt.22564

Cited by 20 publications

(12 citation statements)

References 52 publications

(64 reference statements)

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“…SOD, CAT, and glutathione peroxidase are primary antioxidants involved in the direct ROS mechanism while glutathione is considered as a secondary antioxidant that cooperates in the ROS detoxification via reducing peroxide levels [ 59 , 60 ]. The current study has reported that the levels of various antioxidants including SOD, CAT, GSH have been drastically decreased at disease induction in all groups as evident from previous studies [ 61 , 62 ] and their levels were significantly restored after treatment with the selected plant extracts which is concurrent with the study of Kumar et al [ 63 ]. GPx in the MS individual’s erythrocytes continues undistinguishable meanwhile contradictory data about its activity [ 64 ].…”

Section: Discussionsupporting

confidence: 87%

In Vivo Assessment of the Ameliorative Impact of Some Medicinal Plant Extracts on Lipopolysaccharide-Induced Multiple Sclerosis in Wistar Rats

et al. 2022

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Multiple sclerosis is a chronic autoimmune disorder that leads to the demyelination of nerve fibers, which is the major cause of non-traumatic disability all around the world. Herbal plants Nepeta hindustana L., Vitex negundo L., and Argemone albiflora L., in addition to anti-inflammatory and anti-oxidative effects, have shown great potential as neuroprotective agents. The study was aimed to develop a neuroprotective model to study the effectiveness of herbal plants (N. hindustana, V. negundo, and A. albiflora) against multiple sclerosis. The in vivo neuroprotective effects of ethanolic extracts isolated from N. hindustana, V. negundo, and A. albiflora were evaluated in lipopolysaccharides (LPS) induced multiple sclerosis Wistar rat model. The rat models were categorized into seven groups including group A as normal, B as LPS induced diseased group, while C, D, E, F, and G were designed as treatment groups. Histopathological evaluation and biochemical markers including stress and inflammatory (MMP-6, MDA, TNF-α, AOPPs, AGEs, NO, IL-17 and IL-2), antioxidant (SOD, GSH, CAT, GPx), DNA damage (Isop-2α, 8OHdG) as well as molecular biomarkers (RAGE, Caspase-8, p38) along with glutamate, homocysteine, acetylcholinesterase, and myelin binding protein (MBP) were investigated. The obtained data were analyzed using SPSS version 21 and GraphPad Prism 8.0. The different extract treated groups (C, D, E, F, G) displayed a substantial neuroprotective effect regarding remyelination of axonal terminals and oligodendrocytes migration, reduced lymphocytic infiltrations, and reduced necrosis of Purkinje cells. The levels of stress, inflammatory, and DNA damage markers were observed high in the diseased group B, which were reduced after treatments with plant extracts. The antioxidant activity was significantly reduced in diseased induced group B, however, their levels were raised after treatment with plant extract. Group F (a mélange of all the extracts) showed the most significant change among all other treatment groups (C, D, E, G). The communal dose of selected plant extracts regulates neurodegeneration at the cellular level resulting in restoration and remyelination of axonal neurons. Moreover, 400 mg/kg dose of three plants in conjugation (Group F) were found to be more effective in restoring the normal activities of all measured parameters than independent doses (Group C, D, E) and is comparable with standard drug nimodipine (Group G) clinically used for the treatment of multiple sclerosis. The present study, for the first time, reported the clinical evidence of N. hindustana, V. negundo, and A. albiflora against multiple sclerosis and concludes that all three plants showed remyelination as well neuroprotective effects which may be used as a potential natural neurotherapeutic agent against multiple sclerosis.

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Section: Discussionsupporting

confidence: 87%

In Vivo Assessment of the Ameliorative Impact of Some Medicinal Plant Extracts on Lipopolysaccharide-Induced Multiple Sclerosis in Wistar Rats

et al. 2022

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“…The activities of SOD and CAT were measured using a Nasdox and Nactaz kit (NavandSalamat, Iran), respectively, and according to the kit's instruction. The enzyme activities were expressed as U/mg protein [ 8 , 32 ].…”

Section: Methodsmentioning

confidence: 99%

“…The protein accumulation hypothesis indicated that the accumulation of improperly folded proteins such as amyloid-beta (A β ) and neurofibrillary tangle in the brain of people with Alzheimer's disease cause oxidative stress and inflammatory damage, which ultimately leads to synaptic dysfunction [ 6 ]. Reactive oxygen species (ROS) are free radicals, derived from oxygen, which have been shown to play a role in cell damage [ 7 , 8 ]. They are produced naturally during mitochondrial respiration and energy production, but normally, they are neutralized by the cellular antioxidant defense systems [ 9 – 12 ].…”

Section: Introductionmentioning

confidence: 99%

The Effect of a Traditional Preparation Containing Piper nigrum L. and Bunium persicum (Boiss.) B.Fedtsch. on Immobility Stress-Induced Memory Loss in Mice

Rashedinia

Mojarad

Khodaei

et al. 2021

BioMed Research International

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Objective. Alzheimer’s disease is a progressive, age-related, and neurodegenerative disease characterized by mental decline. The exact cause of Alzheimer’s disease is unclear, but cholinergic dysfunction, protein accumulation, and oxidative stress are among the most important hypotheses. The main purpose of our study was to investigate the effects of aqueous and hydroalcoholic extract combination of these two medicinal plants, black pepper and cumin (as a related formulation in traditional Persian medicine), on memory and learning of an immobilized stress animal model. Methods. In this study, hydroalcoholic and aqueous extracts of cumin and black pepper fruits were prepared. Six groups of mice were treated orally for 2 weeks: control group, immobility stress, and stress-induced immobility mice received different doses of the hydroalcoholic extract (100 and 200 mg/kg) and aqueous extract (100 and 200 mg/kg). The shuttle box, novel object detection, and rotarod test were used to evaluate memory and learning. The activities of acetylcholinesterase, catalase (CAT), and superoxide dismutase (SOD) and the level of reduced glutathione (GSH) and malondialdehyde (MDA) were measured in the brain tissue. Results. Immobility stress significantly reduced learning and motor coordination. Furthermore, MDA levels and acetylcholinesterase activity were significantly increased, while CAT and SOD activities were significantly reduced in the brain of immobility-induced stress mice. Other findings indicated that hydroalcoholic and aqueous extracts (100 and 200 mg/kg) of cumin and black pepper fruits have an improving effect on animal motor coordination and learning ability, GSH content, and CAT, SOD, and acetylcholinesterase enzyme function in comparison with stress groups ( p < 0.05 ). Conclusion. The hydroalcoholic and aqueous extracts of cumin and black pepper fruits have protective effects against stress-induced memory deficit and oxidative stress and may have beneficial therapeutic effect in the treatment of neurodegenerative diseases.

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“…Acute and subchronical neurotoxicity was evaluated through rotarod test, which assesses ataxia and motorial impairment, two signs of neurological damage [ 24 ]. In our study, acute intraperitoneal administrations of all forms of thioctic acid were toxic at 360 and 480 µmol/kg (corresponding to 75 mg/kg and 100 mg/kg of racemic form) because they similarly altered neurological and motorial capabilities of the rodents in all groups of treatment; (+/−) and (+) thioctic acid decreased time of walking at 360 and 480 µmol/kg, while (−) thioctic acid only at 480 µmol/kg.…”

Section: Discussionmentioning

confidence: 99%

Toxicological Profile of the Pain-Relieving Antioxidant Compound Thioctic Acid in Its Racemic and Enantiomeric Forms

et al. 2020

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Thioctic acid is a multipotent antioxidant compound existing as dextrorotatory (+), eutomer and naturally occurring and levorotatory (−). It has been proven to help fight many pathologies and is sold as racemate. In agreement with studies claiming a greater biopotency of the eutomer compared to the levorotatory compound, we recently preclinically and clinically showed that (+) thioctic acid is a pain-reliever as effective as double-dosed racemate. We investigated acute and subchronical toxicity of (+/−) thioctic acid, (−) thioctic acid, (+) thioctic acid and (+) salt thioctic acid on Sprague–Dawley rats. For acute toxicity, compounds were administered intraperitoneally (i.p.) with a single-injection at 125, 240, 360, 480 µmol/kg, then rodents were tested for motorial coordination and minimum lethal dose (LDmin). A subtoxic dose (360 µmol/kg) was administered i.p. for 15 days and we finally evaluated motorial impairment, glycemia, organ toxicity, and apoptosis state. Acutely administered, the highest doses of all thioctic acid compounds negatively affected motorial ability and (−) thioctic acid LDmin resulted higher than the others. Subchronic administrations caused overall body weight loss, motorial impairment, mass loss in some organs. (+/−) and (−) thioctic acid injections enhanced caspase-3 activity in some organs, (−) enantiomer-treated animals displayed more marked organ toxicity signs. Together with our previous study on the biologic role of enantiomers, these data suggest a therapeutic use of (+) enantiomer-based formulations, thus lowering dose and toxicity without affecting the positive effects brought by the drug.

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The effect of ellagic acid on spinal cord and sciatica function in a mice model of multiple sclerosis

Cited by 20 publications

References 52 publications

In Vivo Assessment of the Ameliorative Impact of Some Medicinal Plant Extracts on Lipopolysaccharide-Induced Multiple Sclerosis in Wistar Rats

In Vivo Assessment of the Ameliorative Impact of Some Medicinal Plant Extracts on Lipopolysaccharide-Induced Multiple Sclerosis in Wistar Rats

The Effect of a Traditional Preparation Containing Piper nigrum L. and Bunium persicum (Boiss.) B.Fedtsch. on Immobility Stress-Induced Memory Loss in Mice

Toxicological Profile of the Pain-Relieving Antioxidant Compound Thioctic Acid in Its Racemic and Enantiomeric Forms

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