The effect of epidermal growth factor on the fetal rabbit mandibular condyle and isolated condylar fibroblasts

Tsubai, Takayoshi; Higashi, Youichirou; Scott, J. E.

doi:10.1016/s0003-9969(00)00012-1

Cited by 13 publications

(7 citation statements)

References 29 publications

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“…Both measures indicate an increase in cellular proliferation. 78 The authors also noted the role of EGF in matrix deposition, with tissue volume increasing toward the end of the culture period (21 days). 78 As research moves toward alternative cell sources, our understanding of bioactive signals must be translatable.…”

Section: Current Progress In Tissue Engineeringmentioning

confidence: 95%

“…78 The authors also noted the role of EGF in matrix deposition, with tissue volume increasing toward the end of the culture period (21 days). 78 As research moves toward alternative cell sources, our understanding of bioactive signals must be translatable. Notably, early work by Copray et al 87 demonstrated that most of the factors enhancing proliferation explored in their study, including EGF, similarly enhanced proliferation in secondary mandibular condylar cartilage as well as primary costal chondrocytes.…”

Section: Current Progress In Tissue Engineeringmentioning

confidence: 95%

“…77 In contrast, a second procedure allows the cells to migrate out of the fibrous zone of condylar tissue onto surgical sponges yielding fibroblast-like cells upon isolation. 78 Considering alternative cell sources, most recently, ankle hyaline cartilage cells have been determined to outperform condylar cartilage cells in terms of biosynthesis and cell proliferation when seeded in three dimensional non-woven polyglycolic acid (PGA) meshes, 63 though the authors cited non-adherence of condylar cells as a possible factor in their relatively poor performance. The hyaline cartilage-seeded scaffolds yielded a more fibrocartilaginous tissue with both type I and II collagen.…”

Section: Current Progress In Tissue Engineeringmentioning

confidence: 99%

“…84 An increase in GAG and collagen synthesis, on the other hand, was observed in explants treated with IGF-I alone or in combination with bFGF, with bFGF downregulating IGF-I’s biosynthetic effects when used in combination. Considering next epidermal growth factor (EGF), Tsubai et al, 78 whose isolation technique was previously mentioned, explored EGF treatment in fibroblast-like condylar cells obtained from fetal rats. EGF was shown to bring cells into the s-phase of the cell cycle more quickly and to increase cell number over controls.…”

Section: Current Progress In Tissue Engineeringmentioning

confidence: 99%

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Temporomandibular Disorders: A Review of Etiology, Clinical Management, and Tissue Engineering Strategies

Murphy¹,

MacBarb²,

Wong³

et al. 2013

Int J Oral Maxillofac Implants

314

238

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Epidemiology reports state temporomandibular joint disorders (TMD) affect up to 25% of the population, yet their etiology and progression are poorly understood. As a result, treatment options are limited and fail to meet the long-term demands of the relatively young patient population. TMD are a class of degenerative musculoskeletal conditions associated with morphological and functional deformities. In up to 70% of cases, TMD are accompanied by malpositioning of the TMJ disc, termed “internal derangement.” Though onset is not well characterized, correlations between internal derangement and osteoarthritic change have been identified. Due to the complex and unique nature of each TMD case, diagnosis requires patient-specific analysis accompanied by various diagnostic modalities. Likewise, treatment requires customized plans to address the specific characteristics of each patient’s disease. In the mechanically demanding and biochemically active environment of the TMJ, therapeutic approaches capable of restoring joint functionality while responding to changes in the joint have become a necessity. Capable of integration and adaptation in the TMJ, one such approach, tissue engineering, carries significant potential in the development of repair and replacement tissues. The following review presents a synopsis of etiology, current treatment methods, and the future of tissue engineering for repairing and/or replacing diseased joint components, specifically the mandibular condyle and TMJ disc. Preceding the current trends in tissue engineering is an analysis of native tissue characterization, toward identifying tissue engineering objectives and validation metrics for restoring healthy and functional structures of the TMJ.

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Section: Current Progress In Tissue Engineeringmentioning

confidence: 95%

Section: Current Progress In Tissue Engineeringmentioning

confidence: 95%

Section: Current Progress In Tissue Engineeringmentioning

confidence: 99%

Section: Current Progress In Tissue Engineeringmentioning

confidence: 99%

See 2 more Smart Citations

Temporomandibular Disorders: A Review of Etiology, Clinical Management, and Tissue Engineering Strategies

Murphy¹,

MacBarb²,

Wong³

et al. 2013

Int J Oral Maxillofac Implants

314

238

View full text Add to dashboard Cite

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“…X-ray images were captured using the analysis system from Labtronics (Mississauga, ON, Canada). Quan-titation was done by viewing the X-ray image through a microscope and repetitively measuring the density within a standard area projected over the image of the section using the public domain NIH Image program (developed at the U.S. National Institutes of Health and available on the Internet at http://rsb.info.nih.gov/ nih-image/) (17). Binding was determined by measuring the to- tal counts of each section and dividing this by the area of that section to give mean counts per arbitrary unit area.…”

Section: Ligand-binding Assaysmentioning

confidence: 99%

Identification of Leptin Receptors in Lung and Isolated Fetal Type II Cells

Bergen

Cherlet

Manuel

et al. 2002

Am J Respir Cell Mol Biol

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Leptin is a cytokine involved in regulation of the satiety response. Receptors for this protein have been identified in brain as well as many other peripheral tissues. Some of the highest levels of receptor concentration occur in the lung. Considering the cellular diversity of lung, neither the localization nor the function of leptin in pulmonary tissues has been delineated. The purpose of the present study was to determine if fetal and adult rabbit lung displayed specific binding for leptin, to identify the binding sites, and to explore a potential functional role for leptin in lung surfactant production. Frozen sections of adult and fetal rabbit (24th gestational day) lung were prepared and incubated with increasing concentrations of [125I]leptin in the presence or absence of 1-microM-unlabeled leptin. Sections were removed and radioactivity measured. Concurrently, sections were coated with nuclear Trac emulsion and incubated in the dark at -30 degrees C. Lung showed specific binding for leptin. Microscopically, [125I]leptin was localized to acinar-lining epithelium of developing fetal lung. Larger cells within the epithelial layer appeared to bind leptin more avidly than adjacent cells. Antibodies to the leptin receptor were used to identify binding sites in adult lung and isolated fetal lung type II cells. In adult lung, both the K20 (against the extracellular amino-terminal) and the M18 antibody (against the intracellular carboxy-terminal) displayed several binding sites. In contrast, the isolated fetal type II cells showed only a single binding site for both antibodies. The apparent molecular mass of the receptor using the K20 antibody appeared to be approximately 125 kD. A protein of similar mass bound the M18 antibody suggesting that functional receptor is present in lung and expressed by fetal type II cells. Incubation of isolated fetal type II cells with leptin (0.01-10 microg/ml) stimulated [3H]choline incorporation in disaturated phosphatidylcholine. These results show that fetal and adult lung bind leptin specifically, and fetal type II cells in particular, may be responsive to leptin stimulation of phospholipid production. Leptin may therefore be important in regulating maturation of cells of the fetal lung.

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