The effect of equiaxial stretching on the osteogenic differentiation and mechanical properties of human adipose stem cells

Virjula, Sanni; Zhao, Feihu; Leivo, Joni; Vanhatupa, Sari; Kreutzer, Joose; Vaughan, Ted J.; Honkala, Anna Maija; Viehrig, Marlitt; Mullen, Conleth A.; Kallio, Pasi; McNamara, Laoise M.; Miettinen, Susanna

doi:10.1016/j.jmbbm.2017.04.016

Cited by 23 publications

(14 citation statements)

References 72 publications

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“…The combination of biochemical and mechanical stimulation increases the osteogenic differentiation, when compared to one factor applied solely. Furthermore, stretching increases focal adhesion and the amount of actin in the cytoskeleton [ 143 , 144 ]. This mechanical stimulus has been shown to increase the Wnt signaling, which induces cellular osteogenic differentiation [ 112 ].…”

Section: Osteogenic Stem Cell Differentiation Through Bioactive Factorsmentioning

confidence: 99%

The Role of Adipose Stem Cells in Bone Regeneration and Bone Tissue Engineering

Mende

Götzl

Kubo

et al. 2021

Cells

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Bone regeneration is a complex process that is influenced by tissue interactions, inflammatory responses, and progenitor cells. Diseases, lifestyle, or multiple trauma can disturb fracture healing, which might result in prolonged healing duration or even failure. The current gold standard therapy in these cases are bone grafts. However, they are associated with several disadvantages, e.g., donor site morbidity and availability of appropriate material. Bone tissue engineering has been proposed as a promising alternative. The success of bone-tissue engineering depends on the administered cells, osteogenic differentiation, and secretome. Different stem cell types offer advantages and drawbacks in this field, while adipose-derived stem or stromal cells (ASCs) are in particular promising. They show high osteogenic potential, osteoinductive ability, and immunomodulation properties. Furthermore, they can be harvested through a noninvasive process in high numbers. ASCs can be induced into osteogenic lineage through bioactive molecules, i.e., growth factors and cytokines. Moreover, their secretome, in particular extracellular vesicles, has been linked to fracture healing. The aim of this review is a comprehensive overview of ASCs for bone regeneration and bone tissue engineering.

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Section: Osteogenic Stem Cell Differentiation Through Bioactive Factorsmentioning

confidence: 99%

The Role of Adipose Stem Cells in Bone Regeneration and Bone Tissue Engineering

Mende

Götzl

Kubo

et al. 2021

Cells

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“…Meanwhile, to the best of our knowledge, the anti-tumor activity of mountain cultivated ginseng rust rot has rarely been reported. Besides, it has been proved that the subtle mechanical or morphologic changes of the cells are crucial for the description of cell behaviors (Virjula et al, 2017;Kim et al,2014).…”

Section: A C C E P T E Dmentioning

confidence: 99%

Effect of extract from ginseng rust rot on the inhibition of human hepatocellular carcinoma cells in vitro

Jiao

Wang

et al. 2019

Micron

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“…According to previous studies, the cell is an incompressible material [29, 30]. Thus, we have

λ_{1}^{false(i false)} λ_{2}^{false(i false)} λ_{3}^{false(i false)} = 1

where λ j ( i ) is the principle stretch in the j ‐direction ( j = 1, 2, 3), index i denotes that i = 1 for control group (cardiomyocytes before dosing) and i = 2 for cardiomyocytes after dosing.…”

Section: Characterisation Of Cardiomyocyte Contractionmentioning

confidence: 99%

Cardiomyocyte contractile force changes in response to AGRWE detected by AFM

Zhao²,

Wang

et al. 2019

Micro & Nano Letters

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The cardiac contractile force is an important predictor of healthy and cardiovascular diseases. The changes of cardiomyocyte contractile force in response to American ginseng root water extract (AGRWE) detected by atomic force microscope have not been investigated yet. This study examined the effects of AGRWE on single beating cardiomyocytes extracted from a newborn rat. The same cardiomyocytes were incubated with AGRWE at a concentration of 50 μg/ml for about 30 min, and the cardiomyocytes' contractile force increased from 1.74 ± 1.01 to 3.49 ± 1.53 nN. The mean value of the contractile strain calculated was 3.32 ± 1.55% for the cardiomyocyte before the treatment with AGRWE, while for the cardiomyocyte treated with AGRWE it increased to 4.60 ± 1.35%. The results also showed that the beating rate of the same single beating cardiomyocytes was decreased from 34 ± 11 beats/min (control, n = 10) to 20 ± 9 beats/min. In conclusion, the experimental results have shown clearly that the contractile forces and strain of single beating cardiomyocytes treated with AGRWE are significantly higher than the control group, while the heart rate was decreased. It suggests that ginseng agents are promising candidates in improving cardiac functions for treating heart failure.

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The effect of equiaxial stretching on the osteogenic differentiation and mechanical properties of human adipose stem cells

Cited by 23 publications

References 72 publications

The Role of Adipose Stem Cells in Bone Regeneration and Bone Tissue Engineering

The Role of Adipose Stem Cells in Bone Regeneration and Bone Tissue Engineering

Effect of extract from ginseng rust rot on the inhibition of human hepatocellular carcinoma cells in vitro

Cardiomyocyte contractile force changes in response to AGRWE detected by AFM

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