The effect of ergosterol on the fatty acid composition of Tetrahymena pyriformis

Ferguson, Karen A.; Conner, Robert L.; Mallory, Frank B.

doi:10.1016/0003-9861(71)90501-7

Cited by 30 publications

(11 citation statements)

References 9 publications

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“…An adaptation of the phospholipids to changes in the structure of triterpenoids is seen in Tetrahymena pyriformis. This protozoan changes the fatty acyl groups of phospholipids on transition from a sterol-containing to sterol-free medium by introducing fatty acyl groups with cis-double bonds thus providing the membranes with phospholipids with a larger eros-section (Ferguson et al 1975).…”

Section: Studies With Azole Antifungalsmentioning

confidence: 99%

Importance and Role of Sterols in Fungal Membranes

Bossche

1990

Biochemistry of Cell Walls and Membranes in Fungi

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Section: Studies With Azole Antifungalsmentioning

confidence: 99%

Importance and Role of Sterols in Fungal Membranes

Bossche

1990

Biochemistry of Cell Walls and Membranes in Fungi

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“…Sphingolipids are ubiquitous in eukaryotic fungal, protozoan, animal, and plant cells and can occur in membranes as glycosphingolipids, phosphosphingolipids, and inositolsphingolipids [5][6][7][8][9][10][11][12][13][14][15]. These lipids and their precursors and metabolic products such as sphingosine, sphingosine-1-phosphate, and ceramide can be highly bioactive agents in cell proliferation, differentiation, aging, and apoptosis, transmembrane signal transduction, and other cell functions [16 -19].…”

mentioning

confidence: 99%

Detection and identification of Bacteriovorax stolpii UKi2 sphingophosphonolipid molecular species

Jayasimhulu

Hunt

Kaneshiro

et al. 2007

J. Am. Soc. Mass Spectrom.

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Bacteriovorax stolpii is a predator of larger gram-negative bacteria and lives as a parasite in the intraperiplasmic space of the host cell. This bacterium is unusual among prokaryotes in that sphingolipids comprise a large proportion of its lipids. We here report the presence of 18 molecular species of B. stolpii UKi2 sphingophosphonolipids (SPNLs).31 P NMR spectroscopy and analysis of P i released by a differential hydrolysis protocol confirmed the phosphonyl nature of these lipids. The SPNLs were dominated by those with 1-hydroxy-2-aminoethane phosphonate (hydroxy-aminoethylphosphonate) polar head groups; aminoethylphosphonate was also detected in minor SPNL components. The long-chain bases (LCBs) were dominated by C 17 iso-branched phytosphingosine; C 17 iso-branched dihydrosphingosine was also present in some SPNLs. The N-linked fatty acids were predominantly iso-branched and most contained an ␣-hydroxy group (C 15 ␣-hydroxy fatty acid was the major fatty acid). Minor molecular species containing nonhydroxy fatty acids were also detected. The definitive iso-structures of the predominant fatty acids and LCBs present in the B. stolpii SPNLs were established using 13 C and 3 H nuclear magnetic resonance spectroscopy; less than 20% were unbranched. Detection and analyses of intact compounds by MS-MS were performed by a hybrid quadrupole time-of-flight (Q-TOF-II) MS equipped with an electrospray ionization source. Analyses of peracetylated derivatives verified the structural assignments of these lipids. (J Am Soc Mass Spectrom 2007, 18, 394 -403)

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“…A number of physiological effects of sterol incorporation into ciliate cell membranes have also been reported, e.g. a reduction in cellular volume (as described above) and an elevation in saturated fatty acids (Conner et al 1982;Ferguson et al 1975). Saturated fatty acids are important energy storage molecules.…”

mentioning

confidence: 96%

Supplementation with Sterols Improves Food Quality of a Ciliate for Daphnia magna

Martin‐Creuzburg¹,

Bec²,

Elert³

2006

Protist

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Experimental results provide evidence that trophic interactions between ciliates and Daphnia are constrained by the comparatively low food quality of ciliates. The dietary sterol content is a crucial factor in determining food quality for Daphnia. Ciliates, however, presumably do not synthesize sterols de novo. We hypothesized that ciliates are nutritionally inadequate because of their lack of sterols and tested this hypothesis in growth experiments with Daphnia magna and the ciliate Colpidium campylum. The lipid content of the ciliate was altered by allowing them to feed on fluorescently labeled albumin beads supplemented with different sterols. Ciliates that preyed upon a sterol-free diet (bacteria) did not contain any sterols, and growth of D. magna on these ciliates was poor. Supplementation of the ciliates' food source with different sterols led to the incorporation of the supplemented sterols into the ciliates' cells and to enhanced somatic growth of D. magna. Sterol limitation was thereby identified as the major constraint of ciliate food quality for Daphnia. Furthermore, by supplementation of sterols unsuitable for supporting Daphnia growth, we provide evidence that ciliates as intermediary grazers biochemically upgrade unsuitable dietary sterols to sterols appropriate to meet the physiological demands of Daphnia.

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The effect of ergosterol on the fatty acid composition of Tetrahymena pyriformis

Cited by 30 publications

References 9 publications

Importance and Role of Sterols in Fungal Membranes

Importance and Role of Sterols in Fungal Membranes

Detection and identification of Bacteriovorax stolpii UKi2 sphingophosphonolipid molecular species

Supplementation with Sterols Improves Food Quality of a Ciliate for Daphnia magna

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