The Effect of Erythropoietin on Sodium During Ischemia Reperfusion Injury in Rats

Tsompos, Constantinos

doi:10.4328/jcam.2282

Cited by 9 publications

(13 citation statements)

References 21 publications

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“…Free oxygen radicals released from the ischaemic myocardium may cause damage to many distant organs such as lung, kidney, intestines, and pancreas. Oxidative stress and lipid peroxidation play important roles in distant organ damage after ischaemia/reperfusion (I/R) ( 3 ). Lipid peroxidation seen in diabetic patients further increases the damage to distant organs following reperfusion of the myocardium exposed to ischaemia ( 4 ).…”

mentioning

confidence: 99%

Dexmedetomidine protects from post-myocardial ischaemia reperfusion lung damage in diabetic rats

Kip

Çelik

Bilge

et al. 2015

Libyan Journal of Medicine

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ObjectiveDiabetic complications and lipid peroxidation are known to have a close association. Lipid peroxidation commonly occurs at sites exposed to ischaemia, but distant organs and tissues also get damaged during ischaemia/reperfusion (I/R). Some of these targets are vital organs, such as the lung, liver, and kidney; the lung is the most frequently affected. The aim of our study was to investigate the effects of dexmedetomidine on I/R damage in lung tissue and on the oxidant/anti-oxidant system in diabetic rats.Material and methodsDiabetes was induced with streptozotocin (55 mg/kg) in 18 Wistar Albino rats, which were then randomly divided into three groups (diabetes control (DC), diabetes plus ischaemia-reperfusion (DIR), and diabetes plus dexmedetomidine-ischaemia/reperfusion (DIRD)) after the effects of diabetes were clearly evident. The rats underwent a left thoracotomy and then ischaemia was produced in the myocardium muscle by a left anterior descending artery ligation for 30 min in the DIR and DIRD groups. I/R was performed for 120 min. The DIRD group received a single intraperitoneal dose of dexmedetomidine (100 µg/kg); the DIR group received no dexmedetomidine. Group DC was evaluated as the diabetic control group and also included six rats (C group) in which diabetes was not induced. These mice underwent only left thoracotomy and were closed without undergoing myocardial ischaemia. Histopathological changes, activities of catalase (CAT) and glutathione-S-transferase anti-oxidant enzymes, and malondialdehyde (MDA) levels were evaluated in the lung tissues of all rats.ResultsNeutrophil infiltration/aggregation was higher in the DIR group than in the C, DC, and DIRD groups (p=0.001, p=0.013, and p=0.042, respectively). The lung injury score was significantly higher in the DIR group than in the C and DC groups (p<0.0001 and p=0.024, respectively). The levels of MDA were significantly higher in the DIR group than in the C and DIRD groups. CAT activity was significantly higher in the DIR group than in the DIRD and C groups.ConclusionOur results confirm that dexmedetomidine has protective effects against the lung damage resulting from I/R in diabetic rats. Future studies conducted to evaluate the effects of the use of dexmedetomidine on damage to various organs following different I/R durations may help understanding possible protective effects of dexmedetomidine and underlying mechanisms in tissue damage related to I/R injury.

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mentioning

confidence: 99%

Dexmedetomidine protects from post-myocardial ischaemia reperfusion lung damage in diabetic rats

Kip

Çelik

Bilge

et al. 2015

Libyan Journal of Medicine

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“…Tsompos et al (9) concluded that MPV was associated with acute ischemic stroke severity and it was of high value for discriminating severe from mild ischemic stroke (p=0.012). Tsompos et al (10) identified MPV levels [6.5 fL to 11.7 fL] as an independent predictor of the presence of slow coronary flow (p=0.03). Tsompos et al (11) measured significantly higher MPV values 24 hours after stroke (p=0.001) in patients than in controls on admission.…”

Section: Discussionmentioning

confidence: 99%

The Acute Effect of Erythropoietin on Mean Platelet Volume Levels during Hypoxia Reoxygenation Injury in Rats

Tsompos¹,

Panoulis²,

Toutouzas³

et al. 2016

Haseki

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Ad dressforCor res pon den ce/Ya z›fl maAd re si:Constantinos Tsompos Mesologi County Hospital, Clinic of Obstetrics and Gynecology, Mesologi, Greece E-mail: tsomposconstantinos@gmail.com Received/GeliflTarihi:14 August 2016 Ac cep ted/Ka bulTa ri hi: 16 November 2016 Aim: This biomedical study tested the effect of erythropoietin (Epo) in rat model, especially in a hypoxia-reoxygenation one. The trend of this factor was evaluated hematologically using serum mean platelets volume (MPV) values. Methods:Forty rats with a mean mass 247.7 g were used. MPV values were evaluated at 60 min (for groups A and C) and at 120 min (for groups B and D) of reoxygenation. Epo was provided only in groups C and D.Results:Epo administration non-significantly lowered the MPV levels by 0.12% +2.13% (p=0.9513). Reoxygenation time non-significantly augmented the MPV levels by 1.37%+2.11% (p=0.4873). However, Epo administration together with reoxygenation time non-significantly reduced the MPV levels by 0.27%+0.92% (p=0.7585). Conclusion:Epo administration had a non-significant short-time decline effect on MPV levels. Keywords: Hypoxia, erythropoietin, reoxygenation, mean platelets volumeGiriş:Bu deneysel çalışmada, eritropoietinin (Epo) etkisi fare modelinde, özellikle bir hipoksi-reoksijenasyon protokolü dahilinde incelendi. Bu molekülün etkisi, kan ortalama trombosit düzeyleri (MPV) kullanılarak hematolojik olarak incelendi.Yöntemler:Ortalama 247,7 gr ağırlığa sahip 40 adet fare çalışmada kullanıldı. MPV düzeyleri 60 dakikalık (A ve C grupları) ve 120 dakikalık (B ve D grupları) reoksijenasyonlarla ölçüldü. Sadece C ve D gruplarına Epo verildi. Bulgular: Epo uygulanması MPV düzeylerini kayda değer olmayan bir şekilde %0,12±%2,13 (p=0,9513) oranında yükseltirken reoksijenasyon süresi MPV düzeylerini kayda değer olmayan bir şekilde %1,37±%2,11 (p=0,4873) arttırdı. Ancak, Epo verilmesi ile reoksijenasyon süresi bir araya geldiğinde MPV değerlerini %0,27±%0,92 (p=0,7585) oranında düşürerek ortak bir etki yarattı.Sonuç: Epo uygulamasının MPV düzeyleri üzerinde kayda değer olmayan bir kısa vadeli azalma etkisi olduğu görüldü.

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“…The one variable was that of endosalpingeal karyorrhexis (EK) which was recessed by "without lesions" 0.0181818+0.04393556 (p-value=0.6715) [1]. The other variable was that of salpingitis (S) but did not influence the S scores (p-value=1.0000) [2]. Although Epo is met in over 29,975 published biomedical studies, only a 3.52% of them negotiate its antioxidant capacities.…”

Section: öZmentioning

confidence: 99%

The co-evaluation of endosalpingeal karyorrhexis and salpingitis after the erythropoietin effect on fallopian ischemia reperfusion injury

Tsompos¹,

Constantinos²,

Konstantinos³

et al. 2018

Journal of Surgery and Medicine

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The Effect of Erythropoietin on Sodium During Ischemia Reperfusion Injury in Rats

Cited by 9 publications

References 21 publications

Dexmedetomidine protects from post-myocardial ischaemia reperfusion lung damage in diabetic rats

Dexmedetomidine protects from post-myocardial ischaemia reperfusion lung damage in diabetic rats

The Acute Effect of Erythropoietin on Mean Platelet Volume Levels during Hypoxia Reoxygenation Injury in Rats

The co-evaluation of endosalpingeal karyorrhexis and salpingitis after the erythropoietin effect on fallopian ischemia reperfusion injury

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