The Effect of Ethoxyquin on Tissue Peroxidation and Immune Status of Single Comb White Leghorn Cockerels

Abstract

The responses to supplementing the diet of Single Comb White Leghorn (SCWL) cockerels with ethoxyquin were tested on two parameters: 1) tissue peroxidation and 2) immune response. In the first experiment, three concentrations of supplemental ethoxyquin (0, 500, and 1,000 ppm) were added to a basal diet and fed to SCWL cockerels for 6 wk. Tissue peroxidation was assessed by measuring the thiobarbituric acid reactive substances (TBARS) concentration in the liver, kidney, heart, and spleen. The TBARS concentration in response to 500 ppm dietary ethoxyquin was significantly lower in the liver and spleen tissues, whereas in the kidneys, 1,000 ppm ethoxyquin significantly lowered TBARS. In a second experiment, four concentrations of ethoxyquin (0, 125, 500, and 1,000 ppm) were added to a basal diet and fed to SCWL cockerels for 8 wk. The primary and secondary immune response were assessed by determining antibody titers to the Newcastle disease virus using hemagglutination inhibition (HI) and ELISA. The HI and ELISA titers for the primary and secondary immune response were not significantly different from the control. Analysis of body weight, feed conversion, and organ weight revealed no statistically significant differences between treatments, although in the second experiment the dietary treatment of 1,000 ppm ethoxyquin resulted in significantly higher relative liver weight.

Desempenho e características de carcaça de frangos de corte alimentados com rações contendo farelo da castanha de caju tratado ou não com antioxidante

“…Esses resultados corroboram os descritos por Bailey et al (1996), que não verificaram influência dos níveis de oxidação do óleo testado sobre o peso absoluto do fígado das aves.…”

Safety and efficacy of ethoxyquin (6‐ethoxy‐1,2‐dihydro‐2,2,4‐trimethylquinoline) for all animal species

“…In the absence of vitamin E and ethoxyquin fertility and sperm concentration were reduced; both dosages of ethoxyquin or vitamin E fed after 38 weeks of vitamin E depletion restored fertility and semen concentration to values comparable to males fed vitamin E or ethoxyquin continuously; the lower dosages of vitamin E (32 mg/kg feed) or ethoxyquin maintained fertility and sperm concentration over the 50-week experiment; and no statistically significant differences were observed in semen volume, hatchability of fertile eggs, feed consumption, body weights or dead sperm. Bailey et al (1996) in young chicks from day-old to 6 or 8 weeks of age used up to 1000 mg/kg ethoxyquin in nutritionally adequate diets, and found no signs of toxicity on any health or performance parameter except significantly higher relative liver weights in the groups receiving 1000 mg ethoxyquin/kg feed in the second (8-week) study. The relative liver weights were 2.8 %, 2.7 %, 2.6 % and 3.1 %, in the groups receiving 0, 125, 500 and 1 000 mg ethoxyquin/kg feed.…”

Effect of dried extract of boldo (Peumus Boldus Mol.) on growth and oxidative tissue status of broiler chickens

“…For example, Woodall et al (1996) found no significant differences in weight gain of broiler fed Vit E or several supplementary carotenoids. Jakobsen et al (1994) found no differences in weight gain of poultry supplemented with Vit E. Bailey et al (1996) found no differences in weight gain in poultry whose food had been supplemented with ethoxyquin. In addition, Biswass and Wakita (2001) found that supplementation with green tea polyphenols maintained or lowered weight gain in broilers, and Goñi et al (2007) reported that broilers supplemented with grape pomace exhibited no differences in growth performance.…”