The Effect of Ethyl Pyruvate Supplementation on Rat Fatty Liver Induced by a High-Fat Diet

Olek, Robert A.; Ziółkowski, Wiesław; Flis, Damian Józef; Fedeli, Donatella; Fiorini, Dennis; Wierzba, Tomasz H.; Gabbianelli, Rosita

doi:10.3177/jnsv.59.232

Cited by 11 publications

(7 citation statements)

References 39 publications

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“…In the study reporting an increase of mitochondrial content after long-term HFD, the treatment was initiated at the age of 14 weeks, when the weight of the male Wistar rats was 366 ± 16 g [32]. Consistently, in our study the control rats at the age of 14 weeks were 365 ± 11 g [28], Modification in muscle metabolism may also be influenced by the duration of HFD. The chronological changes in the muscles of rats fed by HFD has been described in the study by Chanseaume et al [6].…”

Section: Discussionsupporting

confidence: 84%

“…Animals had free access to food and water and were kept at room temperature with a light-dark cycle of 12 h. After 6 weeks, both groups were subdivided into 4 groups: control diet (CC; n = 8), control diet and EtP (CP; n = 8), HFD (DC; n = 8), HFD and EtP (DP; n = 8). EtP was administered as 0.3% EtP solution in drinking water for the following 6 weeks [28]. At the end of 12th week, the rats were sacrificed.…”

Section: Methodsmentioning

confidence: 99%

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Effect of Ethyl Pyruvate on Skeletal Muscle Metabolism in Rats Fed on a High Fat Diet

et al. 2013

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Impaired mitochondrial capacity may be implicated in the pathology of chronic metabolic diseases. To elucidate the effect of ethyl pyruvate supplementation on skeletal muscles metabolism we examined changes in activities of mitochondrial and antioxidant enzymes, as well as sulfhydryl groups oxidation (an indirect marker of oxidative stress) during the development of obesity. After 6 weeks feeding of control or high fat diet, Wistar rats were divided into four groups: control diet, control diet and ethyl pyruvate, high fat diet, and high fat diet and ethyl pyruvate. Ethyl pyruvate was administered as 0.3% solution in drinking water, for the following 6 weeks. High fat diet feeding induced the increase of activities 3-hydroxyacylCoA dehydrogenase, citrate synthase, and fumarase. Moreover, higher catalase and superoxide dismutase activities, as well as sulfhydryl groups oxidation, were noted. Ethyl pyruvate supplementation did not affect the mitochondrial enzymes’ activities, but induced superoxide dismutase activity and sulfhydryl groups oxidation. All of the changes were observed in soleus muscle, but not in extensor digitorum longus muscle. Additionally, positive correlations between fasting blood insulin concentration and activities of catalase (p = 0.04), and superoxide dismutase (p = 0.01) in soleus muscle were noticed. Prolonged ethyl pyruvate consumption elevated insulin concentration, which may cause modifications in oxidative type skeletal muscles.

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Section: Discussionsupporting

confidence: 84%

Section: Methodsmentioning

confidence: 99%

Effect of Ethyl Pyruvate on Skeletal Muscle Metabolism in Rats Fed on a High Fat Diet

et al. 2013

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“…EP had received interest as its hepatoprotective effects in fatty liver, hepatic ischemia-reperfusion injury, and acuteon-chronic liver failure [20]. EP downregulated the expression of multiple proinflammatory proteins, including IL-1β, TNF-α, and HMGB1 in animal experiments of endotoxemia and sepsis [21].…”

Section: Discussionmentioning

confidence: 99%

Effects of Ethyl Pyruvate on Bile Duct Ligation-Induced Liver Fibrosis by Regulating Nrf2 Pathway and Proinflammatory Cytokines in Rats

Zong

Zhang

et al. 2019

Gastroenterology Research and Practice

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Aim. The aim of this paper is to investigate the effects of ethyl pyruvate (EP) on experimental liver fibrosis induced by bile duct ligation (BDL) and explore the underlying molecular mechanisms. Material and Method. Rats were randomly divided into three groups: the sham group, the BDL group, and the BDL+EP group. Liver fibrosis was induced by common bile duct ligation and was evaluated by serum biochemical parameter levels, Masson’s trichrome staining, α-SMA expression, and collagen I deposition. The levels of Nrf2 signaling pathway-related antioxidant genes (Nrf2, SOD2, NQO1, and GSH-Px) in liver tissues were also measured. Meanwhile, the mRNA expression levels of HMGB1, IL-1β, TNF-α, and HSP27 were analyzed. In BDL-induced liver fibrosis rats, the successfully established model was confirmed by the significant increase of serum ALT and AST levels, the high liver fibrosis score, α-SMA expression, and collagen deposition. Results. Compared with the BDL group, EP administration could diminish fibrosis level and substantially increase the expression of Nrf2 signaling pathway-related antioxidant genes. Furthermore, EP significantly suppressed the mRNA expression levels of HMGB1, IL-1β, TNF-α, and HSP27. Conclusions. The results suggested that EP administration could effectively inhibit the liver fibrosis induced by BDL in rat, which may be associated with the enhanced activity of Nrf2 to mediate antioxidant enzyme system and downregulate the inflammatory genes.

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“…Previous studies have shown that reduction in liver weight correlates with anti-obesity potential. 22 In few studies, instead of liver weight, the more sensitive % liver weight reduction parameter was used. We did not find reduction in % liver weight as HFD is not a good model to induce changes in liver and the effect of the extract on liver metabolism is negligible.…”

Section: Discussionmentioning

confidence: 99%

To evaluate and compare the efficacy of alcoholic and aqueous extract of Lagenaria siceraria in high fat diet model in wistar rats

Joshi

Tadavi

Birajdar

et al. 2017

Int J Basic Clin Pharmacol

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Background: Obesity is not only affecting the affluent society but also affecting developing countries like India. The incidence of obesity is rapidly increasing throughout the world. However, the current anti-obesity drugs have numerous limitations.Methods: The obesity was induced in male wistar rats by giving high-fat diet over 12 weeks. The variables assessed were body weight, abdominal girth, blood triglyceride level, liver weight and fat mass and histopathology of liver. Aqueous and alcoholic extracts of Lagenaria siceraria (200mg/kg and 400mg/kg Doses) were compared to orlistat (treatment control) and high-fat diet group (disease control) for different variables.Results: Alcoholic and aqueous extracts high dose (400mg/kg) of Lagenaria siceraria significantly reduced total body weight (p<0.05), abdominal girth (p <0.05) at week 10 and 12 compared to high fat diet group. Alcoholic extract (400mg/kg) significantly reduced total blood triglyceride level (p <0.05) and total liver weight (p <0.05) compared to high-fat diet group. None of the study drugs reduced % liver weight. Alcoholic extract high dose (p <0.05) has shown improvement in histopathological score. Both aqueous and alcoholic extracts have shown reduced fat mass compared to high-fat diet group.Conclusions: The alcoholic extract (400mg/kg) of Lagenaria siceraria alleviated high fat diet induced obesity and dyslipidemic changes in rats. The alcoholic extract of Lagenaria siceraria is having better anti-obesity potential than aqueous extract.

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The Effect of Ethyl Pyruvate Supplementation on Rat Fatty Liver Induced by a High-Fat Diet

Cited by 11 publications

References 39 publications

Effect of Ethyl Pyruvate on Skeletal Muscle Metabolism in Rats Fed on a High Fat Diet

Effect of Ethyl Pyruvate on Skeletal Muscle Metabolism in Rats Fed on a High Fat Diet

Effects of Ethyl Pyruvate on Bile Duct Ligation-Induced Liver Fibrosis by Regulating Nrf2 Pathway and Proinflammatory Cytokines in Rats

To evaluate and compare the efficacy of alcoholic and aqueous extract of Lagenaria siceraria in high fat diet model in wistar rats

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